Sequence-specific recognition of double-stranded DNA with molecular beacon with the aid of Ag+ under neutral pH environment

2013 ◽  
Vol 49 (34) ◽  
pp. 3573 ◽  
Author(s):  
Zhiyou Xiao ◽  
Xiaoting Guo ◽  
Liansheng Ling
Keyword(s):  
Molecular Beacon ◽  
Specific Recognition ◽  
Neutral Ph ◽  
Double Stranded Dna

Electrochemical molecular beacon biosensor for sequence-specific recognition of double-stranded DNA

2014 ◽  
Vol 59 ◽  
pp. 54-57 ◽  
Author(s):  
Xiangmin Miao ◽  
Xiaoting Guo ◽  
Zhiyou Xiao ◽  
Liansheng Ling
Keyword(s):  
Molecular Beacon ◽  
Specific Recognition ◽  
Double Stranded Dna

High-specificity double-stranded DNA detection with a “humanoid” molecular beacon and TALEs

Nanoscale ◽  
2018 ◽  
Vol 10 (38) ◽  
pp. 18354-18361 ◽  
Author(s):  
Yu Wang ◽  
Jialei Bai ◽  
Xiaochen Qu ◽  
Yifei Gao ◽  
Jiang Wang ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Dna Detection ◽  
High Specificity ◽  
Fluorescence Signal ◽  
Specific Recognition ◽  
Double Stranded Dna ◽  
Signal Output

Ultra-sensitive and high-specificity detection of dsDNA based on TALEs-specific recognition of DNA properties and H-MB fluorescence signal output capability.

A colorimetric method for the sequence-specific recognition of double-stranded DNA on the surface of a silver-coated glass slide

2018 ◽  
Vol 96 (5) ◽  
pp. 466-470
Author(s):  
Xiaoting Guo ◽  
Jing Wang ◽  
Zhifang Zhu ◽  
Manjun Zhang ◽  
Haigang Li ◽  
...  
Keyword(s):  
Colorimetric Method ◽  
Colour Change ◽  
Glass Slide ◽  
Coated Glass ◽  
Specific Recognition ◽  
Double Stranded Dna ◽  
Pcr Products ◽  
Polymerase Chain ◽  
Good Sequence ◽  
Coated Glass Slide

In this study, a colorimetric method for sequence-specific recognition of double-stranded DNA (dsDNA) was established on the surface of a silver-coated glass slide. Oligo-1 was assembled on the surface of a silver-coated glass slide through an Ag–S bond, and Oligo-2 as reporter was used to bind with streptavidin-horseradish peroxidase (SA–HRP). They could bind with target dsDNA that was composed of Oligo-3 and Oligo-4 on the surface of a silver-coated glass slide through triplex formation. The bound HRP could be moved into the solution by DNase I and catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). Therefore, the concentration of target dsDNA could be determined with the colour change of TMB. Under the optimum conditions, the absorbance was proportional to the concentration of target dsDNA over the range of 100 pmol/L to 2.0 nmol/L, with a detection limit of 13 pmol/L. In addition, this method showed good sequence selectivity, enabling it to be further developed for the detection of other polymerase chain reaction (PCR) products.

Guanosine-rich aptamers@Cu2O nanoparticles: enhanced peroxidase activity and specific recognition capability at neutral pH

2021 ◽  
Author(s):  
Hongchao Ma ◽  
Lan Wang ◽  
Yifan Li ◽  
Yanhui Wei
Keyword(s):  
Peroxidase Activity ◽  
Peroxidase Mimic ◽  
Turn On ◽  
Specific Recognition ◽  
Neutral Ph ◽  
Cu2o Nanoparticles ◽  
Recognition Capability

Decorating Cu2O peroxidase mimic with guanosine-rich aptamers can “turn on” its peroxidase activity at neutral pH media and endow it with specific recognition capability, greatly expanding its application in biological fields.

scholarly journals Sequence-specific recognition of double-stranded DNA by cooperative strand invasion

2006 ◽  
Vol 50 (1) ◽  
pp. 157-158
Author(s):  
Toru Sugiyama ◽  
Yasutada Imamura ◽  
Wataru Hakamata ◽  
Masaaki Kurihara ◽  
Atsushi Kittaka
Keyword(s):  
Specific Recognition ◽  
Double Stranded Dna ◽  
Strand Invasion

Sequence-Specific Recognition of Double-Stranded DNA by Synthetic Minor Groove Binder Conjugates. toward the Construction of Artificial Site-Specific Deoxyribonucleases

2007 ◽  
Vol 26 (10-12) ◽  
pp. 1559-1563
Author(s):  
Alexandre S. Boutorine ◽  
Ludovic Halby ◽  
Philippe Simon ◽  
Loïc Perrouault ◽  
Carine Giovannangeli ◽  
...  
Keyword(s):  
Minor Groove ◽  
Minor Groove Binder ◽  
Site Specific ◽  
Specific Recognition ◽  
Double Stranded Dna

A dumbbell molecular beacon for the specific recognition of nucleic acids

2010 ◽  
Vol 20 (22) ◽  
pp. 6547-6550 ◽  
Author(s):  
Cong Lv ◽  
LiLi Yu ◽  
Jie Wang ◽  
XinJing Tang
Keyword(s):  
Nucleic Acids ◽  
Molecular Beacon ◽  
Specific Recognition

scholarly journals Impact of non-nucleotidic bulges on recognition of mixed-sequence dsDNA by pyrene-functionalized Invader probes

2020 ◽  
Vol 18 (24) ◽  
pp. 4645-4655
Author(s):  
Dale C. Guenther ◽  
Raymond G. Emehiser ◽  
Allison Inskeep ◽  
Saswata Karmakar ◽  
Patrick J. Hrdlicka
Keyword(s):  
Specific Recognition ◽  
Double Stranded Dna ◽  
Mixed Sequence

Invader probes featuring non-nucleotidic bulges are energetically activated for highly specific recognition of complementary double-stranded DNA targets.

Fluorescence recognition of double-stranded DNA based on the quenching of gold nanoparticles to a fluorophore labeled DNA probe

The Analyst ◽  
2016 ◽  
Vol 141 (20) ◽  
pp. 5829-5834 ◽  
Author(s):  
Xiangmin Miao ◽  
Zongbing Li ◽  
Liansheng Ling
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescent Sensor ◽  
Dna Probe ◽  
Specific Recognition ◽  
Double Stranded Dna

This work described an ultrasensitive fluorescent sensor for sequence-specific recognition of dsDNA based on the quenching of gold nanoparticles (AuNPs) to a fluorophore labeled DNA probe.

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