Towards personalised rapid label free miRNA detection for cancer and liver injury diagnostics in cell lysates and blood based samples

Nanoscale ◽  
2018 ◽  
Vol 10 (26) ◽  
pp. 12797-12804 ◽  
Author(s):  
James Duffy ◽  
Francesco Padovani ◽  
Giulio Brunetti ◽  
Peter Noy ◽  
Ulrich Certa ◽  
...  
Keyword(s):  
Liver Injury ◽  
Biological Samples ◽  
Label Free ◽  
Cell Lysates ◽  
Cantilever Arrays ◽  
Mirna Detection

Label free nanomechanical miRNA detection from biological samples utilising cantilever arrays in an automated diagnostic platform.

Polarization label-free microscopy imaging of biological samples by exploiting the Zeeman Laser emission

2021 ◽  
Author(s):  
F. Callegari ◽  
A. Le Gratiet ◽  
A. Zunino ◽  
A. Mohebi ◽  
P. Bianchini ◽  
...  
Keyword(s):  
Biological Samples ◽  
Laser Emission ◽  
Label Free ◽  
Microscopy Imaging ◽  
Zeeman Laser

A novel label-free fluorescence assay for dipeptidyl peptidase 4 activity detection based on supramolecular self-assembly

2020 ◽  
Vol 56 (11) ◽  
pp. 1629-1632
Author(s):  
Yu Zhao ◽  
Jiawen Zou ◽  
Yanyan Song ◽  
Jun Peng ◽  
Yi Wang ◽  
...  
Keyword(s):  
Biological Samples ◽  
Self Assembly ◽  
Dipeptidyl Peptidase ◽  
Fluorescence Assay ◽  
Supramolecular System ◽  
Label Free ◽  
Activity Detection ◽  
Inhibitor Screening ◽  
Dipeptidyl Peptidase 4

A novel supramolecular system CB[7]-GPFG for dipeptidyl peptidase 4 (DPP4) activity detection in biological samples and inhibitor screening.

Optimization and validation of a label-free MRM method for the quantification of cytochrome P450 isoforms in biological samples

2014 ◽  
Vol 406 (20) ◽  
pp. 4861-4874 ◽  
Author(s):  
Ahmad Al Ali ◽  
David Touboul ◽  
Jean-Pierre Le Caër ◽  
Isabelle Schmitz-Afonso ◽  
Jean-Pierre Flinois ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Biological Samples ◽  
Label Free ◽  
Cytochrome P450 Isoforms

Label-free quantification of activated NF-κB in biological samples by atomic force microscopy

2010 ◽  
Vol 25 (11) ◽  
pp. 2490-2496 ◽  
Author(s):  
Michele Menotta ◽  
Rita Crinelli ◽  
Elisa Carloni ◽  
Marzia Bianchi ◽  
Elisa Giacomini ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Biological Samples ◽  
Label Free ◽  
Force Microscopy ◽  
Label Free Quantification ◽  
Atomic Force ◽  
Free Quantification

scholarly journals Nanoparticle sensor for label free detection of swine DNA in mixed biological samples

2011 ◽  
Vol 22 (19) ◽  
pp. 195503 ◽  
Author(s):  
M E Ali ◽  
U Hashim ◽  
S Mustafa ◽  
Y B Che Man ◽  
M H M Yusop ◽  
...  
Keyword(s):  
Biological Samples ◽  
Label Free ◽  
Label Free Detection

Comprehensive Label-Free Method for the Relative Quantification of Proteins from Biological Samples

2005 ◽  
Vol 4 (4) ◽  
pp. 1442-1450 ◽  
Author(s):  
Richard E. Higgs ◽  
Michael D. Knierman ◽  
Valentina Gelfanova ◽  
Jon P. Butler ◽  
John E. Hale
Keyword(s):  
Biological Samples ◽  
Relative Quantification ◽  
Label Free

scholarly journals Multimodal Nonlinear Optical Microscopy for Simultaneous 3-D Label-Free and Immunofluorescence Imaging of Biological Samples

2014 ◽  
Vol 18 (5) ◽  
pp. 551-557
Author(s):  
Joo Hyun Park ◽  
Eun-Soo Lee ◽  
Jae Yong Lee ◽  
Eun Seong Lee ◽  
Tae Geol Lee ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Biological Samples ◽  
Nonlinear Optical ◽  
Label Free ◽  
Nonlinear Optical Microscopy

scholarly journals Label-free and Multimodal Second Harmonic Generation Light Sheet Microscopy

2020 ◽  
Author(s):  
Niall Hanrahan ◽  
Simon I. R. Lane ◽  
Peter Johnson ◽  
Konstantinos Bourdakos ◽  
Christopher Brereton ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Biological Samples ◽  
3D Imaging ◽  
Second Harmonic ◽  
Three Dimensional ◽  
Light Sheet ◽  
Optical Methods ◽  
Label Free ◽  
Light Sheet Microscopy

AbstractLight sheet microscopy (LSM) has emerged as one of most profound three dimensional (3D) imaging tools in the life sciences over the last decade. However, LSM is currently performed with fluorescence detection on one- or multi-photon excitation. Label-free LSM imaging approaches have been rather limited. Second Harmonic Generation (SHG) imaging is a label-free technique that has enabled detailed investigation of collagenous structures, including its distribution and remodelling in cancers and respiratory tissue, and how these link to disease. SHG is generally regarded as having only forward- and back-scattering components, apparently precluding the orthogonal detection geometry used in Light Sheet Microscopy. In this work we demonstrate SHG imaging on a light sheet microscope (SHG-LSM) using a rotated Airy beam configuration that demonstrates a powerful new approach to direct, without any further processing or deconvolution, 3D imaging of harmonophores such as collagen in biological samples. We provide unambiguous identification of SHG signals on the LSM through its wavelength and polarisation sensitivity. In a multimodal LSM setup we demonstrate that SHG and two-photon signals can be acquired on multiple types of different biological samples. We further show that SHG-LSM is sensitive to changes in collagen synthesis within lung fibroblast 3D cell cultures. This work expands on the existing optical methods available for use with light sheet microscopy, adding a further label-free imaging technique which can be combined with other detection modalities to realise a powerful multi-modal microscope for 3D bioimaging.

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