Distinct supramolecular assemblies of Fe(iii) and Ni(ii) complexes constructed from the o-vanillin salicylhydrazone ligand: syntheses, crystal structures, DNA/protein interaction, and antioxidant and cytotoxic activity

2019 ◽  
Vol 43 (21) ◽  
pp. 8024-8043 ◽  
Author(s):  
Yueqin Li ◽  
Yun Li ◽  
Zhiwei Yang ◽  
Fantao Meng ◽  
Nana Wang ◽  
...  
Keyword(s):  
Dna Binding ◽  
Coordination Polymer ◽  
Cytotoxic Activity ◽  
Crystal Structures ◽  
Protein Interaction ◽  
Radical Scavenging ◽  
Dna Binding Protein ◽  
Anticancer Properties ◽  
Dna Protein Interaction

A novel aroylhydrazone ligand-bridged Ni(ii) coordination polymer was reported and its DNA binding, protein interaction, radical scavenging and in vitro anticancer properties were evaluated.

scholarly journals Ni(ii) and Co(ii) complexes of an asymmetrical aroylhydrazone: synthesis, molecular structures, DNA binding, protein interaction, radical scavenging and cytotoxic activity

RSC Advances ◽  
2017 ◽  
Vol 7 (66) ◽  
pp. 41527-41539 ◽  
Author(s):  
Yueqin Li ◽  
Zhiwei Yang ◽  
Minya Zhou ◽  
Yun Li ◽  
Jing He ◽  
...  
Keyword(s):  
Dna Binding ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Protein Interaction ◽  
Binding Protein ◽  
Radical Scavenging ◽  
Dna Binding Protein ◽  
Molecular Structures ◽  
Bsa Binding ◽  
Apoptosis In Cancer Cells

The DNA/BSA binding, antioxidant and cytotoxic activity of Ni(ii) and Co(ii) complexes bearing aroylhydrazone were investigated to induce apoptosis in cancer cells.

Evaluation of DNA Binding, Protein Interaction, and Cytotoxic Activity of a Mononuclear Copper(II) Complex

2013 ◽  
Vol 640 (7) ◽  
pp. 1506-1513 ◽  
Author(s):  
Zhanfen Chen ◽  
Jian Zhang ◽  
Pan Zeng ◽  
Shuping Zhang ◽  
Chuanmin Jin
Keyword(s):  
Dna Binding ◽  
Cytotoxic Activity ◽  
Protein Interaction ◽  
Binding Protein ◽  
Dna Binding Protein

Synthesis, crystal structure of novel unsymmetrical heterocyclic Schiff base Ni(II)/V(IV) complexes: Investigation of DNA binding, protein binding and in vitro cytotoxic activity

2019 ◽  
Vol 488 ◽  
pp. 182-194 ◽  
Author(s):  
Maryam Sedighipoor ◽  
Ali Hossein Kianfar ◽  
Gholamhossein Mohammadnezhad ◽  
Helmar Görls ◽  
Winfried Plass ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Schiff Base ◽  
Dna Binding ◽  
Protein Binding ◽  
Cytotoxic Activity ◽  
Binding Protein ◽  
Dna Binding Protein

scholarly journals DNA–protein interaction studies: a historical and comparative analysis

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Ricardo André Campos Ferraz ◽  
Ana Lúcia Gonçalves Lopes ◽  
Jessy Ariana Faria da Silva ◽  
Diana Filipa Viana Moreira ◽  
Maria João Nogueira Ferreira ◽  
...  
Keyword(s):  
Dna Binding ◽  
Protein Interaction ◽  
Protein Interactions ◽  
Binding Assay ◽  
Historical Context ◽  
Cellular Mechanisms ◽  
Advantages And Disadvantages ◽  
Dna Protein Interaction ◽  
Dna Protein Interactions

Abstract DNA–protein interactions are essential for several molecular and cellular mechanisms, such as transcription, transcriptional regulation, DNA modifications, among others. For many decades scientists tried to unravel how DNA links to proteins, forming complex and vital interactions. However, the high number of techniques developed for the study of these interactions made the choice of the appropriate technique a difficult task. This review intends to provide a historical context and compile the methods that describe DNA–protein interactions according to the purpose of each approach, summarise the respective advantages and disadvantages and give some examples of recent uses for each technique. The final aim of this work is to help in deciding which technique to perform according to the objectives and capacities of each research team. Considering the DNA–binding proteins characterisation, filter binding assay and EMSA are easy in vitro methods that rapidly identify nucleic acid-protein binding interactions. To find DNA-binding sites, DNA-footprinting is indeed an easier, faster and reliable approach, however, techniques involving base analogues and base-site selection are more precise. Concerning binding kinetics and affinities, filter binding assay and EMSA are useful and easy methods, although SPR and spectroscopy techniques are more sensitive. Finally, relatively to genome-wide studies, ChIP–seq is the desired method, given the coverage and resolution of the technique. In conclusion, although some experiments are easier and faster than others, when designing a DNA–protein interaction study several concerns should be taken and different techniques may need to be considered, since different methods confer different precisions and accuracies.

Autoantibodies against a Novel DNA-Binding Protein: DNA-Protein Interaction as a Requisite for Expression of Antigenic Reactivity

1994 ◽  
Vol 72 (2) ◽  
pp. 248-254 ◽  
Author(s):  
Candido Juarez ◽  
Jordi Lluis Vila ◽  
Manuela Agusti ◽  
Carmen Gelpi ◽  
Maria Jose Amengual ◽  
...  
Keyword(s):  
Dna Binding ◽  
Protein Interaction ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Dna Protein Interaction ◽  
Antigenic Reactivity

scholarly journals Synthesis and Characterization of Oxidovanadium(IV) Complexes of 2-((E)-(6-Fluorobenzo[d]thiazol-2-ylimino)methyl)-6-methoxyphenol and Their Antimicrobial, Antioxidant, and DNA-Binding Studies

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
K. Savithri ◽  
H. D. Revanasiddappa
Keyword(s):  
Dna Binding ◽  
Mixed Ligand Complex ◽  
Radical Scavenging ◽  
Molar Conductance ◽  
Diffusion Method ◽  
Spectral Studies ◽  
Binding Studies ◽  
Ligand Complex ◽  
Intercalative Mode

Two novel oxidovanadium(IV) complexes with a new bidentate (O- and N-) imine-based ligand 2-((E)-(6-fluorobenzo[d]thiazol-2-ylimino)methyl)-6-methoxyphenol (HL) were synthesized under in situ experimental condition where VOSO4 acts as a kinetic template in the ratio 2 : 1 (L : M) and mixed ligand complex using 1,10-phenanthroline (phen) in 1 : 1 : 1 (L : M : phen) ratio. The synthesized compounds were structurally characterized by microanalysis, magnetic susceptibility, FTIR, electronic spectra, TG/DTA, ESR, and molar conductance studies. Based on the spectral studies, the complexes have the general composition [VO(L)2] (C1) and [VO(L)phen] (C2) in a square pyramid geometrical fashion. The synthesized compounds were primarily screened for their in vitro growth inhibiting activity against different strains of bacteria, namely, E. coli, B. subtilis, S. aureus, and P. aeruginosa by the disc diffusion method. Also, the antifungal activity was determined against C. albicans and A. niger by the Bateman poisoned technique. The in vitro antioxidant activity of all the compounds was determined by DPPH free radical-scavenging assay. Intercalative mode of DNA-binding properties of the oxidovanadium(IV) complexes with calf-thymus DNA (CT-DNA) was investigated using UV, fluorescence spectra, and viscosity measurements.

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