An integrated microfluidic system for rapid detection and multiple subtyping of influenza A viruses by using glycan-coated magnetic beads and RT-PCR

Lab on a Chip ◽  
2019 ◽  
Vol 19 (7) ◽  
pp. 1277-1286 ◽  
Author(s):  
Kao-Mai Shen ◽  
Narayana Murthy Sabbavarapu ◽  
Chien-Yu Fu ◽  
Jia-Tsrong Jan ◽  
Jen-Ren Wang ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Rapid Detection ◽  
Influenza A ◽  
Magnetic Beads ◽  
Microfluidic System ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Reaction Chambers

A microfluidic chip featuring HA × NA arrayed reaction chambers for RT-PCR was developed for diagnosis and subtyping of influenza A viruses.

Rapid Detection of Eurasian and American H7 Subtype Influenza A Viruses Using a Single Taqmanmgb Real Time RT-PCR

2010 ◽  
Vol 5 (s1) ◽  
pp. e146-e147
Author(s):  
Steven Van Borm ◽  
David L. Suarez ◽  
Marc Boschmans ◽  
Orkun Ozhelvaci ◽  
S. Marché ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Influenza A ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
H7 Subtype

Rapid Detection of Eurasian and American H7 Subtype Influenza A Viruses Using a Single TaqManMGB Real-Time RT-PCR

2010 ◽  
Vol 54 (s1) ◽  
pp. 632-638 ◽  
Author(s):  
Steven Van Borm ◽  
David L. Suarez ◽  
Marc Boschmans ◽  
Orkun Ozhelvaci ◽  
S. Marché ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Influenza A ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
H7 Subtype

scholarly journals Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses

2021 ◽  
pp. 104063872199481
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Hangping Yao ◽  
Nanping Wu ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Real Time ◽  
Influenza A ◽  
Minor Groove Binder ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Pcr Assay ◽  
Infectious Dose ◽  
The Matrix ◽  
Taqman Minor Groove Binder

The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID50)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.

scholarly journals Genomic Signature-Based Identification of Influenza A Viruses Using RT-PCR/Electro-Spray Ionization Mass Spectrometry (ESI-MS) Technology

PLoS ONE ◽  
2010 ◽  
Vol 5 (10) ◽  
pp. e13293 ◽  
Author(s):  
Varough M. Deyde ◽  
Rangarajan Sampath ◽  
Rebecca J. Garten ◽  
Patrick J. Blair ◽  
Christopher A. Myers ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Influenza A ◽  
Genomic Signature ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Esi Ms ◽  
Electro Spray Ionization

Rapid detection for primary screening of influenza A virus: microfluidic RT-PCR chip and electrochemical DNA sensor

The Analyst ◽  
2011 ◽  
Vol 136 (10) ◽  
pp. 2064 ◽  
Author(s):  
Keiichiro Yamanaka ◽  
Masato Saito ◽  
Kenji Kondoh ◽  
Mohammad Mosharraf Hossain ◽  
Ritsuko Koketsu ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Rapid Detection ◽  
Influenza A ◽  
Dna Sensor ◽  
Rt Pcr ◽  
Electrochemical Dna Sensor ◽  
Primary Screening

scholarly journals Development and evaluation of a one-step real-time RT-PCR assay for universal detection of influenza A viruses from avian and mammal species

2010 ◽  
Vol 155 (5) ◽  
pp. 665-673 ◽  
Author(s):  
Alexander Nagy ◽  
Veronika Vostinakova ◽  
Zuzana Pirchanova ◽  
Lenka Cernikova ◽  
Zuzana Dirbakova ◽  
...  
Keyword(s):  
Real Time ◽  
Influenza A ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Pcr Assay ◽  
Mammal Species ◽  
One Step ◽  
Universal Detection

Viral elution and concentration method for detection of influenza A viruses in mud by real-time RT-PCR

2012 ◽  
Vol 179 (1) ◽  
pp. 148-153 ◽  
Author(s):  
Nathalie Deboosere ◽  
Srey Viseth Horm ◽  
Alexandre Delobel ◽  
Jessica Gachet ◽  
Philippe Buchy ◽  
...  
Keyword(s):  
Real Time ◽  
Influenza A ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Concentration Method

scholarly journals Examining the hemagglutinin subtype diversity among wild duck-origin influenza A viruses using ethanol-fixed cloacal swabs and a novel RT-PCR method

Virology ◽  
2008 ◽  
Vol 375 (1) ◽  
pp. 182-189 ◽  
Author(s):  
Ruixue Wang ◽  
Lindsey Soll ◽  
Vivien Dugan ◽  
Jonathan Runstadler ◽  
George Happ ◽  
...  
Keyword(s):  
Influenza A ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Wild Duck ◽  
Pcr Method
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