Rapid detection for primary screening of influenza A virus: microfluidic RT-PCR chip and electrochemical DNA sensor

The Analyst ◽  
2011 ◽  
Vol 136 (10) ◽  
pp. 2064 ◽  
Author(s):  
Keiichiro Yamanaka ◽  
Masato Saito ◽  
Kenji Kondoh ◽  
Mohammad Mosharraf Hossain ◽  
Ritsuko Koketsu ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Rapid Detection ◽  
Influenza A ◽  
Dna Sensor ◽  
Rt Pcr ◽  
Electrochemical Dna Sensor ◽  
Primary Screening

scholarly journals A quantitative RT-PCR assay for rapid detection of Eurasian-lineage H10 subtype influenza A virus

2016 ◽  
Vol 31 (5) ◽  
pp. 444-447 ◽  
Author(s):  
Hailiang Sun ◽  
Jian-Li Xue ◽  
Elizabeth Bailey ◽  
Yifei Xu ◽  
Guoliang Hu ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Rapid Detection ◽  
Influenza A ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Eurasian Lineage

scholarly journals Nested RT-PCR method for the detection of European avian-like H1 swine influenza A virus

2016 ◽  
Vol 15 (5) ◽  
pp. 1095-1102
Author(s):  
Yan-di WEI ◽  
Xing-yao PEI ◽  
Yuan ZHANG ◽  
Chen-fang YU ◽  
Hong-lei SUN ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Swine Influenza ◽  
Rt Pcr ◽  
Pcr Method ◽  
Swine Influenza A Virus

A comprehensive systematic approach to identification of influenza A virus genotype using RT-PCR and RFLP

2000 ◽  
Vol 88 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Daniel P Offringa ◽  
Victoria Tyson-Medlock ◽  
Zhiping Ye ◽  
Roland A Levandowski
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Systematic Approach ◽  
Rt Pcr ◽  
Virus Genotype

scholarly journals One-step real-time RT-PCR for pandemic influenza A virus (H1N1) 2009 matrix gene detection in swine samples

2010 ◽  
Vol 164 (1-2) ◽  
pp. 83-87 ◽  
Author(s):  
Alessio Lorusso ◽  
Kay S. Faaberg ◽  
Mary Lea Killian ◽  
Leo Koster ◽  
Amy L. Vincent
Keyword(s):  
Real Time ◽  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Virus H1n1 ◽  
Rt Pcr ◽  
Gene Detection ◽  
Matrix Gene ◽  
One Step

Comparison of two real-time RT-PCR-based systems for the detection and typing of the pandemic influenza A virus, 2009

2011 ◽  
Vol 177 (2) ◽  
pp. 193-196 ◽  
Author(s):  
Eleonora Lalle ◽  
Licia Bordi ◽  
Silvia Meschi ◽  
Marina Selleri ◽  
Maria Beatrice Valli ◽  
...  
Keyword(s):  
Real Time ◽  
Influenza A Virus ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Rt Pcr

scholarly journals Effectiveness of two rapid influenza tests in comparison to reverse transcription-PCR for influenza A diagnosis

2014 ◽  
Vol 8 (03) ◽  
pp. 331-338 ◽  
Author(s):  
Ramón Zazueta-García ◽  
Adrian Canizalez-Roman ◽  
Hector Flores-Villaseñor ◽  
Javier Martínez-Garcia ◽  
Alejandro Llausas-Vargas ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Correct Diagnosis ◽  
Immunofluorescence Assay ◽  
Immunochromatographic Assay ◽  
High Morbidity ◽  
Rt Pcr ◽  
Predictive Values ◽  
Reverse Transcription Pcr ◽  
Influenza A H1n1

Introduction: The influenza A virus is responsible for high morbidity and mortality in children and adults worldwide. Thus, a rapid, sensitive, and specific diagnosis tool is required. Methodology: An immunofluorescence assay (DFA) and a lateral-flow immunochromatographic assay were compared with RT-PCR for detection of the influenza A virus in 113 nasopharyngeal wash samples obtained from pediatric patients. Samples were collected between July and December 2009, during the pandemic outbreak of influenza A H1N1/09. Results: The sensitivity, specificity, and positive and negative predictive values obtained for the DFA were 68.97%, 76.63%, 75.47%, and 70%, respectively, while the values obtained for the immunochromatographic assay were 58.62%, 81.82%, 77.27%, and 65.22%, respectively. The frequency of the influenza A virus was 51.33%, and a total of 27 samples were positive for the pandemic influenza A H1N1/09. Conclusions: DFA and the immunochromatographic assay can be important tools for patient care during influenza season and in outbreaks as they usually provide results within 45 minutes. Furthermore, positive results in conjunction with the patient’s symptoms could provide a correct diagnosis, thus facilitating appropriate patient management. Nonetheless, the results of these assays still require confirmation by RT-PCR.

scholarly journals Shedding and transmission of a live attenuated influenza A virus vaccine in pre-weaned pigs under field conditions

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0246690
Author(s):  
Gustavo Lopez Moreno ◽  
Jayaveeramuthu Nirmala ◽  
Christa Goodell ◽  
Marie Culhane ◽  
Montserrat Torremorell
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Post Vaccination ◽  
Nasal Swabs ◽  
Common Strategy ◽  
Low Levels ◽  
Live Attenuated Influenza Virus ◽  
Pig Health

Influenza A virus (IAV) is one of the most important respiratory viruses affecting pig health and vaccination is the most common strategy to control influenza infections. In this field study we assessed the onset and duration of shedding of a live attenuated influenza virus (LAIV) vaccine, its ability to transmit to non-vaccinated pigs and whether the LAIV could be aerosolized and detected in the environment. Thirty-three litters (n = 33) of a farm using the LAIV vaccine were selected for the study, a subset of them (n = 12) were left unvaccinated and a subset of piglets (n = 3) in vaccinated litters were also left unvaccinated to serve as sentinels. Selected piglets from the litters were sampled multiple days post vaccination (DPV) by collecting nasal swabs and blood, and were tested using a LAIV vaccine specific RT-PCR assay and hemagglutination inhibition assay against the LAIV strains respectively. Environmental specimens consisting of air and surface wipes were also collected. One hundred percent (21/21) of the vaccinated litters tested LAIV positive 1 DPV and until 6 DPV. In contrast, only five (5/33) of the thirty-three non-vaccinated pigs tested positive during the course of the study. Viable LAIV was confirmed in vaccinated pigs by cell culture and whole genome sequencing. In addition, low levels of LAIV RNA (RT-PCR Ct values ranging between 33 and 38) were detected in all air specimens collected on the day of vaccination and until 6 DPV (3/10). Pigs had maternally derived antibodies reactive against the LAIV strains which may have influenced the degree of shedding observed. Under the conditions of this study, shedding of the LAIV from vaccinated pigs was limited in time, resulted in minimal transmission to non-vaccinated pigs and was detected in low levels in aerosols collected in the vaccinated rooms likely influenced by the presence of maternally derived antibodies against the LAIV strains.

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