scholarly journals Spatiotemporal quantification of acoustic cell patterning using Voronoï tessellation

Lab on a Chip ◽  
2019 ◽  
Vol 19 (4) ◽  
pp. 562-573 ◽  
Author(s):  
James P. K. Armstrong ◽  
Stephanie A. Maynard ◽  
Isaac J. Pence ◽  
Amanda C. Franklin ◽  
Bruce W. Drinkwater ◽  
...  
Keyword(s):  
Image Analysis ◽  
Voronoi Tessellation ◽  
Cell Patterning ◽  
Analysis Method ◽  
Image Analysis Method

A new image analysis method based on Voronoï tessellation that enables quantitative and spatiotemporal characterization of acoustic cell patterning.

Quantification of autolysis inPenicillium chrysogenumby semiautomated image analysis

2001 ◽  
Vol 47 (4) ◽  
pp. 315-321 ◽  
Author(s):  
M McIntyre ◽  
J K Eade ◽  
P W Cox ◽  
C R Thomas ◽  
S White ◽  
...  
Keyword(s):  
Image Analysis ◽  
Previous Method ◽  
Analysis Method ◽  
Image Analysis Method ◽  
Active Biomass ◽  
Additional Information ◽  
Dry Cell Weight ◽  
Automated Measurements ◽  
Dry Cell

An image analysis method is described for the characterization of empty (autolyzed and inactive) regions within the mycelia of filamentous fungi. It extends a previous method that characterized only regions filled with cytoplasm or vacuoles (i.e., the active biomass). The method is semiautomatic, requiring some manual editing before automated measurements. When the method was used for samples from a batch fermentation of an industrial strain of Penicillium chrysogenum, the empty regions were observed to constitute up to 15% (by projected area) of the biomass during the growth phase. After nutrient exhaustion, however, the proportion of empty regions rose rapidly, eventually representing more than 50% of the biomass by the end of fermentation. The increase in the percentage of empty regions coincided with a decrease in biomass (as measured by dry cell weight) and a fall in penicillin titre. Further morphological analysis revealed that fragmentation of mycelia, particularly clumps, coincided with increases in the levels of empty regions. This new image analysis method gave additional information on hyphal differentiation and a measure of autolysis. It was also a useful indicator of the processes leading to autolysis.Key words: autolysis, penicillin, differentiation, double grey.

Advanced digital image analysis method dedicated to the characterization of the morphology of filamentous fungus

2017 ◽  
Vol 266 (2) ◽  
pp. 126-140 ◽  
Author(s):  
N. HARDY ◽  
M. MOREAUD ◽  
D. GUILLAUME ◽  
F. AUGIER ◽  
A. NIENOW ◽  
...  
Keyword(s):  
Image Analysis ◽  
Digital Image ◽  
Filamentous Fungus ◽  
Digital Image Analysis ◽  
Analysis Method ◽  
Image Analysis Method

scholarly journals Automated image analysis method for oil-release test of lipid-based materials

MethodsX ◽  
2021 ◽  
pp. 101447
Author(s):  
Fabio Valoppi ◽  
Petri Lassila ◽  
Ari Salmi ◽  
Edward Haeggström
Keyword(s):  
Image Analysis ◽  
Automated Image Analysis ◽  
Analysis Method ◽  
Image Analysis Method ◽  
Release Test
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