Fabrication of composite microfluidic devices for local control of oxygen tension in cell cultures

Lab on a Chip ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 306-315 ◽  
Author(s):  
Yandong Gao ◽  
Gulnaz Stybayeva ◽  
Alexander Revzin
Keyword(s):  
Oxygen Tension ◽  
Cell Cultures ◽  
Local Control ◽  
Microfluidic Chip ◽  
Gas Permeability ◽  
Microfluidic Devices

We developed a microfabrication strategy that integrated two materials with different gas permeability in a single microfluidic chip to enable local control of oxygen tension for cell cultures.

scholarly journals Recent advances in nonbiofouling PDMS surface modification strategies applicable to microfluidic technology

TECHNOLOGY ◽  
2017 ◽  
Vol 05 (01) ◽  
pp. 1-12 ◽  
Author(s):  
Aslihan Gokaltun ◽  
Martin L. Yarmush ◽  
Ayse Asatekin ◽  
O. Berk Usta
Keyword(s):  
Rapid Prototyping ◽  
Biomedical Applications ◽  
Gas Permeability ◽  
Low Cost ◽  
Microfluidic Devices ◽  
Mature Stage ◽  
Major Drawback ◽  
Pdms Surface ◽  
Recent Advances ◽  
Hydrophobic Recovery

In the last decade microfabrication processes including rapid prototyping techniques have advanced rapidly and achieved a fairly mature stage. These advances have encouraged and enabled the use of microfluidic devices by a wider range of users with applications in biological separations and cell and organoid cultures. Accordingly, a significant current challenge in the field is controlling biomolecular interactions at interfaces and the development of novel biomaterials to satisfy the unique needs of the biomedical applications. Poly(dimethylsiloxane) (PDMS) is one of the most widely used materials in the fabrication of microfluidic devices. The popularity of this material is the result of its low cost, simple fabrication allowing rapid prototyping, high optical transparency, and gas permeability. However, a major drawback of PDMS is its hydrophobicity and fast hydrophobic recovery after surface hydrophilization. This results in significant nonspecific adsorption of proteins as well as small hydrophobic molecules such as therapeutic drugs limiting the utility of PDMS in biomedical microfluidic circuitry. Accordingly, here, we focus on recent advances in surface molecular treatments to prevent fouling of PDMS surfaces towards improving its utility and expanding its use cases in biomedical applications.

scholarly journals Design and application of a microfluidic device for protein crystallization using an evaporation-based crystallization technique

2011 ◽  
Vol 45 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Yong Yu ◽  
Xuan Wang ◽  
Dominik Oberthür ◽  
Arne Meyer ◽  
Markus Perbandt ◽  
...  
Keyword(s):  
Relative Humidity ◽  
Microfluidic Device ◽  
Gas Permeability ◽  
Protein Crystallization ◽  
Microfluidic Devices ◽  
Protein Crystals ◽  
Air Bubbles ◽  
Crystallization System ◽  
Relationship Of

A new crystallization system is described, which makes it possible to use an evaporation-based microfluidic crystallization technique for protein crystallization. The gas and water permeability of the used polydimethylsiloxane (PDMS) material enables evaporation of the protein solution in the microfluidic device. The rates of evaporation are controlled by the relative humidity conditions, which are adjusted in a precise and stable way by using saturated solutions of different reagents. The protein crystals could nucleate and grow under different relative humidity conditions. Using this method, crystal growth could be improved so that approximately 1 mm-sized lysozyme crystals were obtained more successfully than using standard methods. The largest lysozyme crystal obtained reached 1.57 mm in size. The disadvantage of the good gas permeability in PDMS microfluidic devices becomes an advantage for protein crystallization. The radius distributions of aggregrates in the solutions inside the described microfluidic devices were derived fromin situdynamic light scattering measurements. The experiments showed that the environment inside of the microfluidic device is more stable than that of conventional crystallization techniques. However, the morphological results showed that the protein crystals grown in the microfluidic device could lose their morphological stability. Air bubbles in microfluidic devices play an important role in the evaporation progress. A model was constructed to analyze the relationship of the rates of evaporation and the growth of air bubbles to the relative humidity.

scholarly journals Characterization of Nanoparticle Adsorption on Polydimethylsiloxane-Based Microchannels

Sensors ◽  
2021 ◽  
Vol 21 (6) ◽  
pp. 1978
Author(s):  
Hirotada Hirama ◽  
Ryutaro Otahara ◽  
Shinya Kano ◽  
Masanori Hayase ◽  
Harutaka Mekaru
Keyword(s):  
Electrostatic Interactions ◽  
Gas Permeability ◽  
Microfluidic Devices ◽  
Constant Flow ◽  
Constant Flow Rate ◽  
Force Microscopy ◽  
Zeta Potentials ◽  
Atomic Force ◽  
Low Cytotoxicity

Nanoparticles (NPs) are used in various medicinal applications. Exosomes, bio-derived NPs, are promising biomarkers obtained through separation and concentration from body fluids. Polydimethylsiloxane (PDMS)-based microchannels are well-suited for precise handling of NPs, offering benefits such as high gas permeability and low cytotoxicity. However, the large specific surface area of NPs may result in nonspecific adsorption on the device substrate and thus cause sample loss. Therefore, an understanding of NP adsorption on microchannels is important for the operation of microfluidic devices used for NP handling. Herein, we characterized NP adsorption on PDMS-based substrates and microchannels by atomic force microscopy to correlate NP adsorptivity with the electrostatic interactions associated with NP and dispersion medium properties. When polystyrene NP dispersions were introduced into PDMS-based microchannels at a constant flow rate, the number of adsorbed NPs decreased with decreasing NP and microchannel zeta potentials (i.e., with increasing pH), which suggested that the electrostatic interaction between the microchannel and NPs enhanced their repulsion. When exosome dispersions were introduced into PDMS-based microchannels with different wettabilities at constant flow rates, exosome adsorption was dominated by electrostatic interactions. The findings obtained should facilitate the preconcentration, separation, and sensing of NPs by PDMS-based microfluidic devices.

The effect of reduced oxygen tension on progesterone accumulation in rat granulosa cell cultures

Steroids ◽  
1989 ◽  
Vol 54 (5) ◽  
pp. 553-562 ◽  
Author(s):  
Robert D. Koos ◽  
Michael A. Feiertag
Keyword(s):  
Granulosa Cell ◽  
Oxygen Tension ◽  
Cell Cultures

Five percent oxygen tension is not beneficial for neocartilage formation in scaffold-free cell cultures

2012 ◽  
Vol 348 (1) ◽  
pp. 109-117 ◽  
Author(s):  
Chengjuan Qu ◽  
Heli Lindeberg ◽  
Janne H. Ylärinne ◽  
Mikko J. Lammi
Keyword(s):  
Oxygen Tension ◽  
Cell Cultures ◽  
Free Cell

From functional structure to packaging: full-printing fabrication of a microfluidic chip

Lab on a Chip ◽  
2018 ◽  
Vol 18 (13) ◽  
pp. 1859-1866 ◽  
Author(s):  
Fengyi Zheng ◽  
Zhihua Pu ◽  
Enqi He ◽  
Jiasheng Huang ◽  
Bocheng Yu ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Microfluidic Devices ◽  
Functional Structure

This paper presents a concept of a full-printing methodology aiming at convenient and fast fabrication of microfluidic devices.

scholarly journals A novel standalone microfluidic device for local control of oxygen tension for intestinal‐bacteria interactions

2021 ◽  
Vol 35 (2) ◽  
Author(s):  
Chengyao Wang ◽  
Thao Dang ◽  
Jasmine Baste ◽  
Advait Anil Joshi ◽  
Abhinav Bhushan
Keyword(s):  
Oxygen Tension ◽  
Microfluidic Device ◽  
Local Control ◽  
Intestinal Bacteria

An absolute procedure to test the growth potential of medium and the influence of decreased oxygen tension in primary amniotic fluid cell cultures

2006 ◽  
Vol 26 (9) ◽  
pp. 855-860
Author(s):  
Birgit Sikkema-Raddatz ◽  
Ron Suijkerbuijk ◽  
Jakob van der Vlag ◽  
Marian Stoepker ◽  
Charles H. C. M. Buys ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Oxygen Tension ◽  
Cell Cultures ◽  
Growth Potential ◽  
Amniotic Fluid Cell ◽  
Fluid Cell
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