scholarly journals Epitaxially Grown Collagen Fibrils Reveal Diversity in Contact Guidance Behavior among Cancer Cells

Langmuir ◽  
2014 ◽  
Vol 31 (1) ◽  
pp. 307-314 ◽  
Author(s):  
Juan Wang ◽  
Joseph W. Petefish ◽  
Andrew C. Hillier ◽  
Ian C. Schneider
Keyword(s):  
Cancer Cells ◽  
Collagen Fibrils ◽  
Contact Guidance

scholarly journals Transfer of assembled collagen fibrils to flexible substrates for mechanically tunable contact guidance cues

2018 ◽  
Vol 10 (11) ◽  
pp. 705-718 ◽  
Author(s):  
Juan Wang ◽  
Joseph Koelbl ◽  
Anuraag Boddupalli ◽  
Zhiqi Yao ◽  
Kaitlin M. Bratlie ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cancer Invasion ◽  
Collagen Fibrils ◽  
Contact Guidance ◽  
Flexible Substrates ◽  
Guidance Cues ◽  
Aligned Fibers

Contact guidance or bidirectional migration along aligned fibers modulates many physiological and pathological processes such as wound healing and cancer invasion.

Delay of cell cycle progression and induction of death of cancer cells on type I collagen fibrils

2010 ◽  
Vol 52 (3) ◽  
pp. 167-177 ◽  
Author(s):  
Jun Sasaki ◽  
Hitomi Fujisaki ◽  
Eijiro Adachi ◽  
Shinkichi Irie ◽  
Shunji Hattori
Keyword(s):  
Cell Cycle ◽  
Cancer Cells ◽  
Cell Cycle Progression ◽  
Type I Collagen ◽  
Collagen Fibrils ◽  
Type I ◽  
Cycle Progression

A Finite Element Solution for the Anisotropic Biphasic Theory of Tissue-Equivalent Mechanics: The Effect of Contact Guidance on Isometric Cell Traction Measurement

1997 ◽  
Vol 119 (3) ◽  
pp. 261-268 ◽  
Author(s):  
V. H. Barocas ◽  
R. T. Tranquillo
Keyword(s):  
Finite Element Method ◽  
Finite Element ◽  
Mixed Finite Element ◽  
Collagen Fibrils ◽  
Contact Guidance ◽  
Sample Length ◽  
Tissue Equivalent ◽  
Cell Traction ◽  
Anisotropic Biphasic Theory ◽  
Element Method

We present a method for solving the governing equations from our anisotropic biphasic theory of tissue-equivalent mechanics (Barocas and Tranquillo, 1997) for axisymmetric problems. A mixed finite element method is used for discretization of the spatial derivatives, and the DASPK subroutine (Brown et al., 1994) is used to solve the resulting differential-algebraic equation system. The preconditioned GMRES algorithm, using a preconditioner based on an extension of Dembo’s (1994) adaptation of the Uzawa algorithm for viscous flows, provides an efficient and scaleable solution method, with the finite element method discretization being first-order accurate in space. In the cylindrical isometric cell traction assay, the chosen test problem, a cylindrical tissue equivalent is adherent at either end to fixed circular platens. As the cells exert traction on the collagen fibrils, the force required to maintain constant sample length, or load, is measured. However, radial compaction occurs during the course of the assay, so that the cell and network concentrations increase and collagen fibrils become aligned along the axis of the cylinder, leading to cell alignment along the axis. Our simulations predict that cell contact guidance leads to an increase in the load measured in the assay, but this effect is diminished by the tendency of contact guidance to inhibit radial compaction of the sample, which in turn reduces concentrations and hence the measured load.

scholarly journals Mesoscale substrate curvature overrules nanoscale contact guidance to direct bone marrow stromal cell migration

2018 ◽  
Vol 15 (145) ◽  
pp. 20180162 ◽  
Author(s):  
Maike Werner ◽  
Nicholas A. Kurniawan ◽  
Gabriela Korus ◽  
Carlijn V. C. Bouten ◽  
Ansgar Petersen
Keyword(s):  
Bone Marrow ◽  
Cellular Response ◽  
Large Scale ◽  
Biological Tissues ◽  
Collagen Fibrils ◽  
Contact Guidance ◽  
Cylinder Axis ◽  
Cylinder Diameter ◽  
Substrate Curvature ◽  
And Migration

The intrinsic architecture of biological tissues and of implanted biomaterials provides cells with large-scale geometrical cues. To understand how cells are able to sense and respond to complex structural environments, a deeper insight into the cellular response to multi-scale and conflicting geometrical cues is needed. In this study, we subjected human bone marrow stromal cells (hBMSCs) to mesoscale cylindrical surfaces (diameter 250–5000 µm) and nanoscale collagen fibrils (diameter 100–200 nm) that were aligned perpendicular to the cylinder axis. On flat surfaces and at low substrate curvatures (cylinder diameter d > 1000 µm), cell alignment and migration were governed by the nanoscale collagen fibrils, consistent with the contact guidance effect. With increasing surface curvature (decreasing cylinder diameter, d < 1000 µm), cells increasingly aligned and migrated along the cylinder axis, i.e. the direction of zero curvature. An increase in phosphorylated myosin light chain levels was observed with increasing substrate curvature, suggesting a link between substrate-induced cell bending and the F-actin–myosin machinery. Taken together, this work demonstrates that geometrical cues of up to 10× cell size can play a dominant role in directing hBMSC alignment and migration and that the effect of nanoscale contact guidance can even be overruled by mesoscale curvature guidance.

scholarly journals Modeling Contact Guidance and Invasion by Cancer Cells

2014 ◽  
Vol 74 (17) ◽  
pp. 4588-4596 ◽  
Author(s):  
Leonard M. Sander
Keyword(s):  
Cancer Cells ◽  
Contact Guidance

scholarly journals The mechanics and dynamics of cancer cells sensing noisy 3D contact guidance

2021 ◽  
Vol 118 (10) ◽  
pp. e2024780118
Author(s):  
Jihan Kim ◽  
Yuansheng Cao ◽  
Christopher Eddy ◽  
Youyuan Deng ◽  
Herbert Levine ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cell Morphology ◽  
Principal Direction ◽  
Three Dimensional ◽  
Contact Guidance ◽  
Directional Bias ◽  
Cell Responses ◽  
Migration Dynamics ◽  
3D Collagen ◽  
And Migration

Contact guidance is a major physical cue that modulates cancer cell morphology and motility, and is directly linked to the prognosis of cancer patients. Under physiological conditions, particularly in the three-dimensional (3D) extracellular matrix (ECM), the disordered assembly of fibers presents a complex directional bias to the cells. It is unclear how cancer cells respond to these noncoherent contact guidance cues. Here we combine quantitative experiments, theoretical analysis, and computational modeling to study the morphological and migrational responses of breast cancer cells to 3D collagen ECM with varying degrees of fiber alignment. We quantify the strength of contact guidance using directional coherence of ECM fibers, and find that stronger contact guidance causes cells to polarize more strongly along the principal direction of the fibers. Interestingly, sensitivity to contact guidance is positively correlated with cell aspect ratio, with elongated cells responding more strongly to ECM alignment than rounded cells. Both experiments and simulations show that cell–ECM adhesions and actomyosin contractility modulate cell responses to contact guidance by inducing a population shift between rounded and elongated cells. We also find that cells rapidly change their morphology when navigating the ECM, and that ECM fiber coherence modulates cell transition rates between different morphological phenotypes. Taken together, we find that subcellular processes that integrate conflicting mechanical cues determine cell morphology, which predicts the polarization and migration dynamics of cancer cells in 3D ECM.

Ultrastructural modification of cellular interactions in cancer tumor

1978 ◽  
Vol 36 (2) ◽  
pp. 318-319
Author(s):  
N. P. Dmitrieva
Keyword(s):  
Cancer Cells ◽  
Human Thyroid ◽  
Adjacent Cell ◽  
Main Role ◽  
Cellular Interactions ◽  
Electron Microscopic ◽  
Cancer Tumor ◽  
Normal Human ◽  
Characteristic Features

One of the most characteristic features of cancer cells is their ability to metastasia. It is suggested that the modifications of the structure and properties of cancer cells surfaces play the main role in this process. The present work was aimed at finding out what ultrastructural features apear in tumor in vivo which removal of individual cancer cells from the cell population can provide. For this purpose the cellular interactions in the normal human thyroid and cancer tumor of this gland electron microscopic were studied. The tissues were fixed in osmium tetroxide and were embedded in Araldite-Epon.In normal human thyroid the most common type of intercellular contacts was represented by simple junction formed by the parallelalignment of adjacent cell membranees leaving in between an intermembranes space 15-20 nm filled with electronlucid material (Fig. 1a). Sometimes in the basal part of cells dilatations of the intercellular space 40-50 nm wide were found (Fig. 1a). Here the cell surfaces may form single short microvilli.

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