Plasmid binding to metal oxide nanoparticles inhibited lateral transfer of antibiotic resistance genes

2019 ◽  
Vol 6 (5) ◽  
pp. 1310-1322 ◽  
Author(s):  
Xiaojie Hu ◽  
Bing Yang ◽  
Wei Zhang ◽  
Chao Qin ◽  
Xue Sheng ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Metal Oxide ◽  
Resistance Genes ◽  
Metal Oxide Nanoparticles ◽  
Antibiotic Resistance Genes ◽  
Lateral Transfer ◽  
Oxide Nanoparticles

Aggregates or adducts of MONPs and plasmids are blocked out by cells, resulting in the inhibition of lateral plasmid-mediated ARG transfer.

scholarly journals Positive and Negative Effects of Metal Oxide Nanoparticles on Antibiotic Resistance Genes Transfer

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 742
Author(s):  
Georgy D. Otinov ◽  
Alina V. Lokteva ◽  
Anastasia D. Petrova ◽  
Irina V. Zinchenko ◽  
Maria V. Isaeva ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Gene Transfer ◽  
Metal Oxide ◽  
Resistance Genes ◽  
Rapid Development ◽  
Metal Oxide Nanoparticles ◽  
Antibiotic Resistance Genes ◽  
Biological Synthesis ◽  
Oxide Nanoparticles ◽  
E Coli

Rapid development of antibiotic resistance in bacteria is a critical public health problem in the world. One of the main routes of resistance development is the transfer of genes containing antibiotic resistance cassettes. Gene transfer can be done through horizontal transfer of genes: transduction, conjugation, and transformation. Many factors in the environment influence these processes, and one of them is the action of metal oxide nanoparticles (MONPs), which can appear in the milieu through both biological synthesis and the release of engineered nanomaterial. In this study, the effect of AlOOH, CuO, Fe3O4, TiO2, and ZnO MONPs on the transformation (heat shock transformation) of bacteria Escherichia coli K12, and the conjugation between E. coli cc118 and E. coli Nova Blue were studied. The MONPs were synthesized by one method and fully characterized. ZnO nanoparticles (NPs) have significantly increased the efficiency of transformation (more than 9-fold), while the other NPs have reduced it to 31 times (TiO2 NPs). AlOOH NPs increased the number of transconjugants more than 1.5-fold, while CuO and Fe3O4 NPs did not have a significant effect on transformation and conjugation. Thus, the data shows that different types of MONPs can enhance or inhibit different gene transfer mechanisms, affecting the spread of antibiotic resistance genes.

scholarly journals Metal Oxide Nanoparticles Against Bacterial Biofilms: Perspectives and Limitations

2020 ◽  
Vol 8 (10) ◽  
pp. 1545 ◽  
Author(s):  
Liubov Shkodenko ◽  
Ilia Kassirov ◽  
Elena Koshel
Keyword(s):  
Antibiotic Resistance ◽  
Comparative Analysis ◽  
Metal Oxide ◽  
Metal Oxide Nanoparticles ◽  
Antimicrobial Properties ◽  
Bacterial Biofilms ◽  
Oxide Nanoparticles ◽  
Promising Solution ◽  
Low Efficiency ◽  
Al2o3 Nps

At present, there is an urgent need in medicine and industry to develop new approaches to eliminate bacterial biofilms. Considering the low efficiency of classical approaches to biofilm eradication and the growing problem of antibiotic resistance, the introduction of nanomaterials may be a promising solution. Outstanding antimicrobial properties have been demonstrated by nanoparticles (NPs) of metal oxides and their nanocomposites. The review presents a comparative analysis of antibiofilm properties of various metal oxide NPs (primarily, CuO, Fe3O4, TiO2, ZnO, MgO, and Al2O3 NPs) and nanocomposites, as well as mechanisms of their effect on plankton bacteria cells and biofilms. The potential mutagenicity of metal oxide NPs and safety problems of their wide application are also discussed.

Rare earth oxide nanoparticles promote soil microbial antibiotic resistance by selectively enriching antibiotic resistance genes

2019 ◽  
Vol 6 (2) ◽  
pp. 456-466 ◽  
Author(s):  
Lin Qi ◽  
Yuan Ge ◽  
Tian Xia ◽  
Ji-Zheng He ◽  
Congcong Shen ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Rare Earth ◽  
Microbial Communities ◽  
Resistance Genes ◽  
Soil Microbial Communities ◽  
Antibiotic Resistance Genes ◽  
Rare Earth Oxide ◽  
Oxide Nanoparticles ◽  
Soil Microbial

This study demonstrates that rare earth oxide nanoparticles can enhance soil microbial antibiotic resistance by inducing the enrichment and spread of antibiotic resistance genes in soil microbial communities.

scholarly journals The Evolution of Class 1 Integrons and the Rise of Antibiotic Resistance

2008 ◽  
Vol 190 (14) ◽  
pp. 5095-5100 ◽  
Author(s):  
Michael Gillings ◽  
Yan Boucher ◽  
Maurizio Labbate ◽  
Andrew Holmes ◽  
Samyuktha Krishnan ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Lateral Transfer ◽  
Natural Environments ◽  
Class 1 Integrons ◽  
Wide Range ◽  
Human Food Chain ◽  
Class 1 ◽  
Phylogenetic Signature

ABSTRACT Class 1 integrons are central players in the worldwide problem of antibiotic resistance, because they can capture and express diverse resistance genes. In addition, they are often embedded in promiscuous plasmids and transposons, facilitating their lateral transfer into a wide range of pathogens. Understanding the origin of these elements is important for the practical control of antibiotic resistance and for exploring how lateral gene transfer can seriously impact on, and be impacted by, human activities. We now show that class 1 integrons can be found on the chromosomes of nonpathogenic soil and freshwater Betaproteobacteria. Here they exhibit structural and sequence diversity, an absence of antibiotic resistance genes, and a phylogenetic signature of lateral transfer. Some examples are almost identical to the core of the class 1 integrons now found in pathogens, leading us to conclude that environmental Betaproteobacteria were the original source of these genetic elements. Because these elements appear to be readily mobilized, their lateral transfer into human commensals and pathogens was inevitable, especially given that Betaproteobacteria carrying class 1 integrons are common in natural environments that intersect with the human food chain. The strong selection pressure imposed by the human use of antimicrobial compounds then ensured their fixation and global spread into new species.

Quintuplex PCR to Detect Antibiotic Resistance Genes in Streptococcus pneumoniae

2016 ◽  
Vol 1 (2) ◽  
pp. 22 ◽  
Author(s):  
Navindra Kumari Palanisamy ◽  
Parasakthi Navaratnam ◽  
Shamala Devi Sekaran
Keyword(s):  
Antibiotic Resistance ◽  
Streptococcus Pneumoniae ◽  
Resistance Genes ◽  
Bacterial Pathogen ◽  
Antibiotic Resistance Genes ◽  
Pcr Amplification ◽  
Penicillin Resistance ◽  
Penicillin Binding Proteins ◽  
Efflux Mechanism ◽  
Mic Value

Introduction: Streptococcus pneumoniae is an important bacterial pathogen, causing respiratory infection. Penicillin resistance in S. pneumoniae is associated with alterations in the penicillin binding proteins, while resistance to macrolides is conferred either by the modification of the ribosomal target site or efflux mechanism. This study aimed to characterize S. pneumoniae and its antibiotic resistance genes using 2 sets of multiplex PCRs. Methods: A quintuplex and triplex PCR was used to characterize the pbp1A, ermB, gyrA, ply, and the mefE genes. Fifty-eight penicillin sensitive strains (PSSP), 36 penicillin intermediate strains (PISP) and 26 penicillin resistance strains (PRSP) were used. Results: Alteration in pbp1A was only observed in PISP and PRSP strains, while PCR amplification of the ermB or mefE was observed only in strains with reduced susceptibility to erythromycin. The assay was found to be sensitive as simulated blood cultures showed the lowest level of detection to be 10cfu. Conclusions: As predicted, the assay was able to differentiate penicillin susceptible from the non-susceptible strains based on the detection of the pbp1A gene, which correlated with the MIC value of the strains.

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