Validation of a quantitative PCR based detection system for indoor mold exposure assessment in bioaerosols

2018 ◽  
Vol 20 (10) ◽  
pp. 1454-1468 ◽  
Author(s):  
Verena Unterwurzacher ◽  
Clara Pogner ◽  
Harald Berger ◽  
Joseph Strauss ◽  
Sabine Strauss-Goller ◽  
...  
Keyword(s):  
Air Quality ◽  
Exposure Assessment ◽  
Quantitative Pcr ◽  
Detection System ◽  
Data Normalization ◽  
Indoor Mold ◽  
Final Data ◽  
Mold Exposure ◽  
Quality Assessments ◽  
Parallel Measurements

Validation of a newly developed qPCR based detection system showed that sample spiking, parallel measurements of known references and final data normalization are crucial for reliability and possible comparison of air quality assessments addressing indoor mold.

Derivation of motor vehicle tailpipe particle emission factors suitable for modelling urban fleet emissions and air quality assessments

2009 ◽  
Vol 17 (3) ◽  
pp. 724-739 ◽  
Author(s):  
Diane U. Keogh ◽  
Joe Kelly ◽  
Kerrie Mengersen ◽  
Rohan Jayaratne ◽  
Luis Ferreira ◽  
...  
Keyword(s):  
Air Quality ◽  
Motor Vehicle ◽  
Emission Factors ◽  
Particle Emission ◽  
Quality Assessments

scholarly journals A web-based screening tool for near-port air quality assessments

2017 ◽  
Vol 98 ◽  
pp. 21-34 ◽  
Author(s):  
Vlad Isakov ◽  
Timothy M. Barzyk ◽  
Elizabeth R. Smith ◽  
Saravanan Arunachalam ◽  
Brian Naess ◽  
...  
Keyword(s):  
Air Quality ◽  
Screening Tool ◽  
Web Based ◽  
Quality Assessments

Adverse health effects of indoor mold exposure

2006 ◽  
Vol 118 (3) ◽  
pp. 763-763 ◽  
Author(s):  
A LIEBERMAN ◽  
W REA ◽  
L CURTIS
Keyword(s):  
Health Effects ◽  
Adverse Health Effects ◽  
Adverse Health ◽  
Indoor Mold ◽  
Mold Exposure

184 EXPRESSION OF GENES RELATED TO DNA METHYLATION AND GLUCOSE METABOLISM DURING THE PRE-IMPLANTATIONAL STAGE OF BOVINE EMBRYOS

2011 ◽  
Vol 23 (1) ◽  
pp. 193
Author(s):  
A. R. Ferreira ◽  
G. M. Machado ◽  
J. M. Azevedo ◽  
R. Sartori ◽  
M. A. N. Dode ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Glucose Metabolism ◽  
Quantitative Pcr ◽  
Target Genes ◽  
Bos Taurus ◽  
Detection System ◽  
Rna Extraction ◽  
Developmental Phase ◽  
Cell Stage

During pre-implantation embryonic development in mammals, extensive epigenetic reprogramming occurs; patterns of DNA methylation are erased during the first cell divisions and are restored at the 8- to 16-cell stage (Dean et al. 2001). An important step during this developmental phase, which is controlled by epigenetic factors such as DNA methylation, is inactivation of the X chromosome (XCI). It is established in the morula stage, where the paternal X is inactivated, with immediate reactivation in the blastocyst (Ferreira et al. 2010). The aim of this study was to evaluate the mRNA expression profile of the DNMT3a and DNMT3b genes, which are related to DNA methylation, and the G6PD and PGK1 genes, which are related to glucose metabolism and are subject to XCI. Cumulus–oocyte complexes (COC) were isolated from slaughterhouse ovaries from Bos taurus indicus Nellore cows. Oocytes were matured in vitro and inseminated with X-sorted sperm from a Holstein bull. Embryos of each developmental stage [4-cell (44 hpi), 8- to 16-cell (72 hpi), morula (144 hpi), blastocyst (156 hpi), and expanded blastocyst (168 hpi)] were produced. Three pools of 17 embryos for each stage were frozen at –80°C until RNA extraction. Total RNA was isolated using the Invisorb RNA Spin Cell Mini Kit (Invitek, Berlin, Germany) according to the manufacturer’s protocol. Complementary DNA was done using Oligo dT primers (Invitrogen, São Paulo, Brazil) and SuperScript III reverse transcriptase (Invitrogen). Relative expression of target genes was assessed by quantitative PCR using a Power Sybr®Green PCR Master Mix (Applied Biosystems, Foster City, CA) in an ABI Prism 7500 Fast Sequence Detection System (Applied Biosystems). The cyclophilin-A gene was used as an endogenous control. To compare gene expression between treatments, we used the ΔΔCt method. The identity of PCR products was confirmed by the amplicon size in agarose gel and by the melt curve in quantitative PCR. Gene quantification was compared among treatments using one-way ANOVA and Tukey’s test, in the Prophet program, version 5.0 (1996, BBN Systems and Technologies, Cambridge, MA). The 4 genes showed higher expression (P ≤ 0.05) in 4-cell stage embryos than in morulae, blastocysts, or expanded blastocysts. The 8- to 16-cell embryos showed no differences for any gene compared with other embryos, except for the DNMT3b gene, which was not tested in these embryos. We conclude that these genes have a greater quantity of mRNA molecules because of the presence of maternal mRNA and that this stock was being spent during embryo development until the expanded blastocyst stage. Financial support was provided by Embrapa and CNPq.

scholarly journals Comparison of indoor air sampling and dust collection methods for fungal exposure assessment using quantitative PCR

2017 ◽  
Vol 19 (10) ◽  
pp. 1312-1319 ◽  
Author(s):  
Jennie Cox ◽  
Reshmi Indugula ◽  
Stephen Vesper ◽  
Zheng Zhu ◽  
Roman Jandarov ◽  
...  
Keyword(s):  
Exposure Assessment ◽  
Quantitative Pcr ◽  
Indoor Air ◽  
Sampling Methods ◽  
Air Sampling ◽  
Fungal Contamination ◽  
Dust Collection ◽  
Fungal Exposure ◽  
The Many ◽  
Collection Methods

Evaluating fungal contamination indoors is complicated because of the many different sampling methods utilized.

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