A cationic organoiridium(iii) complex-based AIEgen for selective light-up detection of rRNA and nucleolar staining

2018 ◽  
Vol 47 (33) ◽  
pp. 11477-11490 ◽  
Author(s):  
Sanjoy Kumar Sheet ◽  
Bhaskar Sen ◽  
Kripamoy Aguan ◽  
Snehadrinarayan Khatua
Keyword(s):  
Cell Imaging ◽  
Imaging Experiment ◽  
Nucleolar Staining

Cyclometalated Ir(iii) complex-based AIEgen has been developed to selectively detect and stain the cell rRNA which has been revealed by in vitro PL studies and cell imaging experiment.

KLa(0.95−x)GdxF4:Eu3+ hexagonal phase nanoparticles as luminescent probes for in vitro Huh-7 cancer cell imaging

2021 ◽  
Vol 50 (15) ◽  
pp. 5197-5207
Author(s):  
Mohini Gupta ◽  
Rajamani Nagarajan ◽  
Chitteti Ramamurthy ◽  
Perumal Vivekanandan ◽  
G. Vijaya Prakash
Keyword(s):  
Liver Cancer ◽  
Cancer Cell ◽  
Cell Imaging ◽  
Hexagonal Phase ◽  
Liver Cancer Cell ◽  
Luminescent Probes ◽  
Cancer Cell Imaging ◽  
Site Selective

Strong and site selective red-emitting photoluminescent/MRI multi-functional KLa(0.95−x)GdxF4:Eu3+ (x = 0–0.4) bio-compatible nanomaterials for targeted in-vitro liver cancer cell imaging.

Fluorescent carbon dots from milk by microwave cooking

RSC Advances ◽  
2016 ◽  
Vol 6 (47) ◽  
pp. 41516-41521 ◽  
Author(s):  
Dan Wang ◽  
Lin Zhu ◽  
Christopher Mccleese ◽  
Clemens Burda ◽  
Jian-Feng Chen ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Cell Imaging ◽  
Two Photon ◽  
Microwave Cooking ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Fluorescent carbon dots were synthesized from milk by microwave cooking and used for two-photon excitedin vitrocell imaging.

Multicolor (Vis-NIR) mesoporous silica nanospheres linked with lanthanide complexes using 2-(5-bromothiophen)imidazo[4,5-f][1,10]phenanthroline for in vitro bioimaging

2015 ◽  
Vol 44 (1) ◽  
pp. 237-246 ◽  
Author(s):  
Ying Liu ◽  
Lining Sun ◽  
Jinliang Liu ◽  
Yu-Xin Peng ◽  
Xiaoqian Ge ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Lanthanide Complexes ◽  
Cell Imaging ◽  
Silica Nanospheres ◽  
Phenanthroline Ligand ◽  
Mesoporous Silica Nanospheres

Using the modified phenanthroline ligand, the multicolor mesoporous silica nanospheres linked with lanthanide complexes were synthesized and characterized, and the application for cell imaging has been studied.

I2 catalyzed access of spiro[indoline-3,4′-pyridine] appended amine dyad: new ON–OFF chemosensors for Cu2+ and imaging in living cells

2018 ◽  
Vol 16 (2) ◽  
pp. 302-315 ◽  
Author(s):  
Animesh Mondal ◽  
Barnali Naskar ◽  
Sanchita Goswami ◽  
Chandraday Prodhan ◽  
Keya Chaudhuri ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hepg2 Cells ◽  
Cell Imaging ◽  
Colorimetric Detection ◽  
Living Cells ◽  
Fluorescent Cell

An efficient, easily tuneable route to construct a structurally diverse organic fluorescent probe and its applications towards the colorimetric detection of Cu2+ ions and in vitro fluorescent cell imaging of Cu2+ in HepG2 cells.

A new visible-light-excitable ICT-CHEF-mediated fluorescence ‘turn-on’ probe for the selective detection of Cd2+ in a mixed aqueous system with live-cell imaging

2015 ◽  
Vol 44 (12) ◽  
pp. 5763-5770 ◽  
Author(s):  
Shyamaprosad Goswami ◽  
Krishnendu Aich ◽  
Sangita Das ◽  
Chitrangada Das Mukhopadhyay ◽  
Deblina Sarkar ◽  
...  
Keyword(s):  
Visible Light ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Aqueous System ◽  
Live Cell ◽  
Selective Detection ◽  
Turn On ◽  
Fluorescence Turn On

A new quinoline based sensor was developed and applied for the selective detection of Cd2+ both in vitro and in vivo.

scholarly journals tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies

2021 ◽  
Author(s):  
Y. Bousmah ◽  
H. Valenta ◽  
G. Bertolin ◽  
U. Singh ◽  
V. Nicolas ◽  
...  
Keyword(s):  
Single Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Yellow Fluorescent Protein ◽  
Resonance Energy ◽  
Live Cell ◽  
Live Cells

AbstractYellow fluorescent proteins (YFP) are widely used as optical reporters in Förster Resonance Energy Transfer (FRET) based biosensors. Although great improvements have been done, the sensitivity of the biosensors is still limited by the low photostability and the poor fluorescence performances of YFPs at acidic pHs. In fact, today, there is no yellow variant derived from the EYFP with a pK1/2 below ∼5.5. Here, we characterize a new yellow fluorescent protein, tdLanYFP, derived from the tetrameric protein from the cephalochordate B. lanceolatum, LanYFP. With a quantum yield of 0.92 and an extinction coefficient of 133 000 mol−1.L.cm−1, it is, to our knowledge, the brightest dimeric fluorescent protein available, and brighter than most of the monomeric YFPs. Contrasting with EYFP and its derivatives, tdLanYFP has a very high photostability in vitro and preserves this property in live cells. As a consequence, tdLanYFP allows the imaging of cellular structures with sub-diffraction resolution with STED nanoscopy. We also demonstrate that the combination of high brightness and strong photostability is compatible with the use of spectro-microscopies in single molecule regimes. Its very low pK1/2 of 3.9 makes tdLanYFP an excellent tag even at acidic pHs. Finally, we show that tdLanYFP can be a FRET partner either as donor or acceptor in different biosensing modalities. Altogether, these assets make tdLanYFPa very attractive yellow fluorescent protein for long-term or single-molecule live-cell imaging that is also suitable for FRET experiment including at acidic pH.

scholarly journals BMP4-induced symmetry breaking in a 3D model of the human epiblast

2019 ◽  
Author(s):  
Mijo Simunovic ◽  
Ali H. Brivanlou ◽  
Eric D. Siggia
Keyword(s):  
Live Cell Imaging ◽  
3D Model ◽  
Epithelial To Mesenchymal Transition ◽  
Cell Imaging ◽  
Embryonic Stem ◽  
Primitive Streak ◽  
Mesenchymal Transition ◽  
Pluripotency Markers ◽  
Anterior Posterior

Abstract We describe the protocol of generating a 3D stem-cell-based model of the human pre-gastrulation epiblast by culturing human embryonic stem cells in a mix of hydrogel and Matrigel. Much like the epiblast of an in vitro attached day-10 human embryo, this model is an epithelial sphere with a cavity at its center, it is expressing key pluripotency markers, and it displays apico-basal polarity. The 3D colonies can further be differentiated with morphogens and in the case of intermediate concentrations of BMP4, they break the anterior-posterior symmetry characterized by an asymmetric expression of a primitive streak marker and showing signs of epithelial to mesenchymal transition. The protocol described here is suitable for immunofluorescence staining and for live-cell imaging.

scholarly journals Rapid Morphological and Cytoskeletal Response to Microgravity in Human Primary Macrophages

2019 ◽  
Vol 20 (10) ◽  
pp. 2402 ◽  
Author(s):  
Cora Sandra Thiel ◽  
Svantje Tauber ◽  
Beatrice Lauber ◽  
Jennifer Polzer ◽  
Christian Seebacher ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Imaging ◽  
Live Cell ◽  
Cellular Structures ◽  
Microgravity Environment ◽  
Confocal Laser ◽  
Human Macrophages ◽  
Imaging Experiment

The FLUMIAS (Fluorescence-Microscopic Analyses System for Life-Cell-Imaging in Space) confocal laser spinning disk fluorescence microscope represents a new imaging capability for live cell imaging experiments on suborbital ballistic rocket missions. During the second pioneer mission of this microscope system on the TEXUS-54 suborbital rocket flight, we developed and performed a live imaging experiment with primary human macrophages. We simultaneously imaged four different cellular structures (nucleus, cytoplasm, lysosomes, actin cytoskeleton) by using four different live cell dyes (Nuclear Violet, Calcein, LysoBrite, SiR-actin) and laser wavelengths (405, 488, 561, and 642 nm), and investigated the cellular morphology in microgravity (10−4 to 10−5 g) over a period of about six minutes compared to 1 g controls. For live imaging of the cytoskeleton during spaceflight, we combined confocal laser microscopy with the SiR-actin probe, a fluorogenic silicon-rhodamine (SiR) conjugated jasplakinolide probe that binds to F-actin and displays minimal toxicity. We determined changes in 3D cell volume and surface, nuclear volume and in the actin cytoskeleton, which responded rapidly to the microgravity environment with a significant reduction of SiR-actin fluorescence after 4–19 s microgravity, and adapted subsequently until 126–151 s microgravity. We conclude that microgravity induces geometric cellular changes and rapid response and adaptation of the potential gravity-transducing cytoskeleton in primary human macrophages.

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