Light-up RNA aptamer enabled label-free protein detection via a proximity induced transcription assay

2018 ◽  
Vol 54 (64) ◽  
pp. 8877-8880 ◽  
Author(s):  
Zhan-Ming Ying ◽  
Hu-Yan Xiao ◽  
Hao Tang ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang
Keyword(s):  
Signal Amplification ◽  
Protein Detection ◽  
Rna Aptamer ◽  
Label Free ◽  
Free Protein ◽  
Transcription Assay ◽  
Highly Sensitive ◽  
Dna Template

A novel proximity induced transcription assay for highly sensitive protein detection based on protein mediated ligation of a DNA template with the transcription of a light-up RNA aptamer for signal amplification has been developed.

scholarly journals Highly sensitive and multiplexed in situ protein profiling with cleavable fluorescent streptavidin

2019 ◽  
Author(s):  
Renjie Liao ◽  
Diego Mastroeni ◽  
Paul D. Coleman ◽  
Jia Guo
Keyword(s):  
Signal Amplification ◽  
Individual Cell ◽  
Protein Detection ◽  
Protein Profiling ◽  
Detection Sensitivity ◽  
Layer By Layer ◽  
Protein Targets ◽  
Highly Sensitive ◽  
Wide Range

AbstractThe ability to perform highly sensitive and multiplexed in situ protein analysis is crucial to advance our understanding of normal physiology and disease pathogenesis. To achieve this goal, here we develop an approach using cleavable biotin conjugated antibodies and cleavable fluorescent streptavidin (CFS). In this approach, protein targets are first recognized by the cleavable biotin labeled antibodies. Subsequently, CFS is applied to stain the protein targets. Though layer-by-layer signal amplification using cleavable biotin conjugated orthogonal antibodies and CSF, the protein detection sensitivity can be enhanced by at least 10 fold, compared with the existing methods. After imaging, the fluorophores and the biotins unbound to streptavidin are removed by chemical cleavage. The leftover streptavidin is blocked by biotin. Upon reiterative analysis cycles, a large number of different proteins with a wide range of expression levels can be unambiguously detected in individual cell in situ.

scholarly journals Optimization of the Electrodeposition of Gold Nanoparticles for the Application of Highly Sensitive, Label-Free Biosensor

Biosensors ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 50 ◽  
Author(s):  
Hao-Chun Chiang ◽  
Yanyan Wang ◽  
Qi Zhang ◽  
Kalle Levon
Keyword(s):  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Aqueous Electrolyte ◽  
Label Free ◽  
Reduction Step ◽  
Glucose Biosensors ◽  
Highly Sensitive ◽  
Amperometric Glucose Biosensors ◽  
Aunp Surface

A highly sensitive electrochemical biosensor with a signal amplification platform of electrodeposited gold nanoparticle (AuNP) has been developed and characterized. The sizes of the synthesized AuNP were found to be critical for the performance of biosensor in which the sizes were dependent on HAuCl4 and acid concentrations; as well as on scan cycles and scan rates in the gold electro-reduction step. Systematic investigations of the adsorption of proteins with different sizes from aqueous electrolyte solution onto the electrodeposited AuNP surface were performed with a potentiometric method and calibrated by design of experiment (DOE). The resulting amperometric glucose biosensors was demonstrated to have a low detection limit (> 50 μM) and a wide linear range after optimization with AuNP electrodeposition.

An electrochemical impedance biosensor with aptamer-modified pyrolyzed carbon electrode for label-free protein detection

2008 ◽  
Vol 129 (1) ◽  
pp. 372-379 ◽  
Author(s):  
Jung A Lee ◽  
Seongpil Hwang ◽  
Juhyoun Kwak ◽  
Se Il Park ◽  
Seung S. Lee ◽  
...  
Keyword(s):  
Electrochemical Impedance ◽  
Protein Detection ◽  
Carbon Electrode ◽  
Label Free ◽  
Free Protein

An Optical Nanoruler Based on a Conjugated Polymer−Silver Nanoprism Pair for Label-Free Protein Detection

2015 ◽  
Vol 27 (39) ◽  
pp. 6040-6045 ◽  
Author(s):  
Xiaoyu Wang ◽  
Shengliang Li ◽  
Pengbo Zhang ◽  
Fengting Lv ◽  
Libing Liu ◽  
...  
Keyword(s):  
Conjugated Polymer ◽  
Protein Detection ◽  
Label Free ◽  
Free Protein

Protein Detection: An Optical Nanoruler Based on a Conjugated Polymer−Silver Nanoprism Pair for Label-Free Protein Detection (Adv. Mater. 39/2015)

2015 ◽  
Vol 27 (39) ◽  
pp. 6039-6039 ◽  
Author(s):  
Xiaoyu Wang ◽  
Shengliang Li ◽  
Pengbo Zhang ◽  
Fengting Lv ◽  
Libing Liu ◽  
...  
Keyword(s):  
Conjugated Polymer ◽  
Protein Detection ◽  
Label Free ◽  
Free Protein

Label-free protein detection using a microfluidic Coulter-counter device

2014 ◽  
Vol 190 ◽  
pp. 922-927 ◽  
Author(s):  
Romen Rodriguez-Trujillo ◽  
Mohammad A. Ajine ◽  
Alice Orzan ◽  
Mikkel D. Mar ◽  
Flemming Larsen ◽  
...  
Keyword(s):  
Protein Detection ◽  
Coulter Counter ◽  
Label Free ◽  
Free Protein

scholarly journals Functionalized etched tilted fiber Bragg grating aptasensor for label-free protein detection

2019 ◽  
Vol 146 ◽  
pp. 111765 ◽  
Author(s):  
Marzhan Sypabekova ◽  
Sanzhar Korganbayev ◽  
Álvaro González-Vila ◽  
Christophe Caucheteur ◽  
Madina Shaimerdenova ◽  
...  
Keyword(s):  
Fiber Bragg Grating ◽  
Protein Detection ◽  
Bragg Grating ◽  
Label Free ◽  
Free Protein

Direct-writing colloidal photonic crystal microfluidic chips by inkjet printing for label-free protein detection

Lab on a Chip ◽  
2012 ◽  
Vol 12 (17) ◽  
pp. 3089 ◽  
Author(s):  
Weizhi Shen ◽  
Mingzhu Li ◽  
Changqing Ye ◽  
Lei Jiang ◽  
Yanlin Song
Keyword(s):  
Photonic Crystal ◽  
Inkjet Printing ◽  
Protein Detection ◽  
Microfluidic Chips ◽  
Label Free ◽  
Direct Writing ◽  
Free Protein
Sign in / Sign up

Export Citation Format

Share Document