Intra-hydrogel culture prevents transformation of mesenchymal stem cells induced by monolayer expansion

2018 ◽  
Vol 6 (5) ◽  
pp. 1168-1176 ◽  
Author(s):  
Tongmeng Jiang ◽  
Junting Liu ◽  
Yiqiang Ouyang ◽  
Huayu Wu ◽  
Li Zheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Signaling Pathway ◽  
Focal Adhesion ◽  
Cellular Transformation ◽  
Mapk Signaling ◽  
Mapk Signaling Pathway ◽  
Monolayer Expansion

Intra-hydrogel culture can mitigate the cellular transformation of MSCs induced by expansion through the regulation of proteoglycans in cancer (PGC) and pathways in cancer (PC) focal adhesion (FA) and the MAPK signaling pathway.

scholarly journals Strontium Promotes Osteogenic Differentiation of Mesenchymal Stem Cells Through the Ras/MAPK Signaling Pathway

2009 ◽  
Vol 23 (1-3) ◽  
pp. 165-174 ◽  
Author(s):  
Songlin Peng ◽  
Guangqian Zhou ◽  
Keith D.K. Luk ◽  
Kenneth M.C. Cheung ◽  
Zhaoyang Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Mapk Signaling ◽  
Mapk Signaling Pathway

CKIP-1 contributes to osteogenic differentiation of mouse bone marrow mesenchymal stem cells

2021 ◽  
Vol 16 (9) ◽  
pp. 847-859
Author(s):  
Qiannan Niu ◽  
Shuning Shen ◽  
Jiaojiao He ◽  
Lei Wang
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Mouse Model ◽  
Signaling Pathway ◽  
Mapk Signaling ◽  
Mapk Signaling Pathway ◽  
Negative Regulator ◽  
Mouse Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

Background: Osteogenesis greatly depends on the differentiation of bone marrow mesenchymal stem cells (BMSCs). CKIP-1 is considered to be a negative regulator of BMSCs. Methods: We established a  CKIP-1 knockout mouse model, then isolated and cultured BMSCs from wild-type and knockout groups. Results: Our data demonstrated that CKIP-1 knockout significantly increased bone structure in the experimental mouse model and enhanced BMSC proliferation. CKIP-1 knockout contributed to osteoblastic and adipogenic differentiation. Furthermore, CKIP-1 regulated osteogenesis in BMSCs via the MAPK signaling pathway, and BMSCs from the  CKIP-1 knockout mice were effective in repairing the skull defect null mice. Conclusion: Our results concluded that silencing of CKIP-1 promoted osteogenesis in experimental mice and increased BMSCs differentiation via upregulation of the MAPK signaling pathway.

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