Indirect competitive ELISA and colloidal gold-based immunochromatographic strip for amantadine detection in animal-derived foods

2019 ◽  
Vol 11 (15) ◽  
pp. 2027-2032
Author(s):  
Mingfei Pan ◽  
Jingying Yang ◽  
Shijie Li ◽  
Guozhu Wang ◽  
Junping Wang ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Colloidal Gold ◽  
Antiviral Drug ◽  
Competitive Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Strip ◽  
Indirect Competitive Elisa

In this research, an indirect competitive enzyme-linked immunosorbent assay (ELISA) and colloidal gold-based immunochromatographic (GICG) strip were developed for the detection of the antiviral drug amantadine (AM) in animal-derived foods.

Production of a monoclonal antibody for the detection of vitamin B1 and its use in an indirect enzyme-linked immunosorbent assay and immunochromatographic strip

2020 ◽  
Vol 8 (9) ◽  
pp. 1935-1943 ◽  
Author(s):  
Lu Zeng ◽  
Xaioling Wu ◽  
Liqiang Liu ◽  
Liguang Xu ◽  
Hua Kuang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Colloidal Gold ◽  
Vitamin B1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vitamin B ◽  
Immunochromatographic Test ◽  
Immunochromatographic Strip

A monoclonal antibody (mAb) against vitamin B1 was prepared and based on this, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and colloidal gold-based immunochromatographic test (ICT) strip were developed.

Rapid quantitative determination of fentanyl in human urine and serum using a gold-based immunochromatographic strip sensor

2020 ◽  
Vol 8 (37) ◽  
pp. 8573-8584
Author(s):  
Xianlu Lei ◽  
Xinxin Xu ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Liguang Xu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quantitative Determination ◽  
Immunosorbent Assay ◽  
Human Urine ◽  
Colloidal Gold ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Strip ◽  
Human Urine And Serum ◽  
Urine And Serum

In this study, an ultrasensitive monoclonal antibody (mAb) was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold-based immunochromatographic strip (CG-ICS) for the analysis of fentanyl in urine and serum.

scholarly journals Determination of Azadirachtin in Agricultural Matrixes and Commercial Formulations by Enzyme-Linked Immunosorbent Assay

2001 ◽  
Vol 84 (4) ◽  
pp. 1001-1010 ◽  
Author(s):  
Kalidindi Hemalatha ◽  
Namburi B K Venugopal ◽  
Namburi B K Venugopal ◽  
Beedu S Rao
Keyword(s):  
Immunosorbent Assay ◽  
Cross Reactivity ◽  
Competitive Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Agricultural Commodities ◽  
Indirect Competitive Elisa ◽  
Capture Assay ◽  
Ic50 Value ◽  
Antibody Capture

Abstract An enzyme-linked immunosorbent assay (ELISA) was developed for azadirachtin (aza), a biopesticide from the neem tree (Azadirachta indica A. Juss). The immunogen was synthesized by epoxidation using the furan ring in the aza molecule. Rabbits were immunized with either bovine serum albumin (BSA)-azadirachtin or ovalbumin (OA)-azadirachtin conjugate. Evaluation of the antisera by antibody capture assay showed that the antibody titer of antisera raised against OA-aza was 1:30 000. An indirect competitive ELISA was developed with BSA-azadirachtin as coating antigen and aza-specific antibodies raised against OA-aza immunogen. The immunoassay showed an inhibitory concentration (IC50) value of 75 ppb, with a range of detection from 0.5 to 1000 ppb for azadirachtin [based on regression analysis, y = 85.87 (−18.89x); r2 = −0.97]. Cross-reactivity of the antibodies with 2 aza- derivatives (22,23-dihydro-23β-methoxy azadirachtin and 3-tigloylazadirachtol) was 33 and 29%, respectively. The indirect competitive ELISA was validated and evaluated by quantitating aza in spiked agricultural commodities and from neem formulations. Azadirachtin was spiked into 5 different agricultural commodities: tomato, brinjal, coffee, tea, and cotton seed at 500 and 1000 ppb and recovered at 62–100%. In samples drawn from 6 lots, the aza content in neem-seed kernels ranged from 0.1 to 0.15%; in commercial neem formulations the content ranged from 200 to 2000 ppm. The method developed may be applied to environmental monitoring of aza and quality assurance studies of aza-based commercial formulations.

scholarly journals Quantitation of Aflatoxin B1-N7-Guanine Adduct in Urine by Enzyme-Linked Immunosorbent Assay Coupled with Immunoaffinity Chromatography

1997 ◽  
Vol 80 (5) ◽  
pp. 1013-1022 ◽  
Author(s):  
Tfflrumurthy Vidyasagar ◽  
Vadapalli Vyjayanthi ◽  
Nayak Sujatiia ◽  
Beedu Sashidhar Rao ◽  
Ramesh Venkataramana Bhat
Keyword(s):  
Immunosorbent Assay ◽  
Aflatoxin B1 ◽  
Polyclonal Antibodies ◽  
Immunoaffinity Chromatography ◽  
Competitive Elisa ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunoaffinity Column ◽  
Indirect Competitive Elisa

Abstract IndiaA specific and sensitive method to quantitate aflatoxin B1-N7-guanine adduct in urine samples by immunoaffinity chromatography coupled with indirect competitive enzyme-linked immunosorbent assay (ELISA) is reported. A novel in vitro method to synthesize an antigen (bovine serum albumin-guanine-aflatoxin B1) and its use to produce polyclonal antibodies specific to the hapten aflatoxin B-N7-guanine are discussed. An indirect competitive ELISA developed to quantitate aflatoxin adduct showed a 50% inhibition at 15.6 pmol aflatoxin B1-N7-guanine (y=66.73+ (-19.8)x, r=-0.997). Interference by aflatoxin B1 was less than 5%, and no interference by guanine, aflatoxin Gι and aflatoxin M1 was observed. An immunoaffinity column was also developed, by using these polyclonal antibodies, for single-step purification of aflatoxin B1-N7-gua-nine. The immunoaffinity column and the indirect competitive ELISA were evaluated and validated by quantitation of aflatoxin B1-N7-guanine in urine from rats dosed with aflatoxin B1 (1 mg/kg body weight). Spiking studies with standard aflatoxin B1-N7-guanine adduct at 2 and 4 μg/mL phosphate- buffered saline gave 96 and 100% recoveries, respectively, for immunoaffinity cleanup column. The method also was tested successfully for quantitating aflatoxin B1-N7-guanine adduct in spiked human urine. Recoveries of the adduct were 79-90%.

scholarly journals Biotinylated single-chain variable fragment-based enzyme-linked immunosorbent assay for glycocholic acid

The Analyst ◽  
2018 ◽  
Vol 143 (9) ◽  
pp. 2057-2065 ◽  
Author(s):  
Xiping Cui ◽  
Natalia Vasylieva ◽  
Ding Shen ◽  
Bogdan Barnych ◽  
Jun Yang ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Competitive Elisa ◽  
Scfv Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Indirect Competitive Elisa ◽  
Glycocholic Acid

An indirect competitive ELISA was developed for GCA detection based on biotinylated scFv antibody.

Preparation of an anti-formoterol monoclonal antibody for indirect competitive ELISA detection of formoterol in urine and pork samples

2018 ◽  
Vol 10 (5) ◽  
pp. 548-553 ◽  
Author(s):  
Li Chunsheng ◽  
Li Yujing ◽  
Zhang Yan ◽  
Liu Jingjing ◽  
Li Junhua ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Competitive Elisa ◽  
Urine Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Indirect Competitive Elisa ◽  
Pork Samples

In this study, an enzyme-linked immunosorbent assay (ELISA) was established to detect formoterol (FMT) residue in pork and urine samples.

Application of ELISA to Retail Survey of Aflatoxin B1 in Peanut Butter

1986 ◽  
Vol 49 (10) ◽  
pp. 792-795 ◽  
Author(s):  
BHANU P. RAM ◽  
L. PATRICK HART ◽  
RICHARD J. COLE ◽  
JAMES J. PESTKA
Keyword(s):  
Immunosorbent Assay ◽  
Aflatoxin B1 ◽  
Coefficient Of Variation ◽  
Simple Procedure ◽  
Peanut Butter ◽  
Competitive Elisa ◽  
Routine Screening ◽  
Enzyme Linked Immunosorbent Assay

A simple procedure was devised for the routine screening of aflatoxin B1 (AFB1) in peanut butter using enzyme-linked immunosorbent assay (ELISA). Peanut butter samples (5 g) were artificially contaminated with AFB1 and extracted by blending with 25 ml of 55% methanol and 10 ml of hexane. The extract was filtered and aqueous filtrate analyzed by a direct competitive ELISA. Recovery of AFB1 added to peanut butter samples ranged from 85 to 112%, with an average inter-well coefficient of variation of 18.4%. The inter-assay coefficient of variation was 22.7%. Using this procedure, only 3 of 63 commercial samples of peanut butter had detectable levels (>5.0 μg/kg) of AFB1.

Detection of total bile acids in biological samples using an indirect competitive ELISA based on four monoclonal antibodies

2017 ◽  
Vol 9 (4) ◽  
pp. 625-633 ◽  
Author(s):  
Shuchen Liu ◽  
Yue Zhang ◽  
Baoping Qu ◽  
Gaofeng Qin ◽  
Jinjun Cheng ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Clinical Practice ◽  
Biological Samples ◽  
Competitive Elisa ◽  
Routine Clinical Practice ◽  
Enzyme Linked Immunosorbent Assay ◽  
Indirect Competitive Elisa ◽  
Different Types ◽  
Total Bile Acids

We investigated a newly developed indirect competitive enzyme-linked immunosorbent assay for the determination of 5 major components of TBA, which works efficiently in different types of biological samples, and may be suitable for routine clinical practice.

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