Preparation of an anti-formoterol monoclonal antibody for indirect competitive ELISA detection of formoterol in urine and pork samples

2018 ◽  
Vol 10 (5) ◽  
pp. 548-553 ◽  
Author(s):  
Li Chunsheng ◽  
Li Yujing ◽  
Zhang Yan ◽  
Liu Jingjing ◽  
Li Junhua ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Competitive Elisa ◽  
Urine Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Indirect Competitive Elisa ◽  
Pork Samples

In this study, an enzyme-linked immunosorbent assay (ELISA) was established to detect formoterol (FMT) residue in pork and urine samples.

A Competitive Enzyme-Linked Immunosorbent Assay for Detection of Bovine Milk in Ovine and Caprine Milk and Cheese Using a Monoclonal Antibody against Bovine β-Casein

1997 ◽  
Vol 60 (1) ◽  
pp. 64-66 ◽  
Author(s):  
G. ANGUITA ◽  
R. MARTÍN ◽  
T. GARCÍA ◽  
P. MORALES ◽  
A. I. HAZA ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Binding Sites ◽  
Bovine Milk ◽  
Microtiter Plate ◽  
Competitive Elisa ◽  
Quantitative Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Caprine Milk ◽  
Mouse Immunoglobulin

A competitive ELISA (enzyme-linked immunosorbent assay) was performed to detect and quantify bovine milk in ovine and caprine milk and cheese using a monoclonal antibody (AH4 MAb) against bovine β-casein. Ovine or caprine milk and cheese containing bovine milk were added simultaneously with the AH4 MAb to the wells of a microtiter plate that had been previously sensitized with commercial bovine β-casein. The bovine caseins in milk or cheese samples compete with the bovine β-casein bound to the plate for the AH4 MAb binding sites. Further immunorecognition of AH4 MAb bound to the bovine β-casein immobilized onto the plate was attained with rabbit anti-mouse immunoglobulin conjugated to peroxidase. Subsequent enzymic conversion of the substrate showed clear differences in absorbance values during assay of mixtures of ovine and caprine milk and cheese containing various amounts of bovine milk. The competitive ELISA developed in this work allows the quantitative detection of bovine milk in ovine and caprine milk and cheese samples in the range of 0.5 to 25% of substitution.

scholarly journals Determination of Azadirachtin in Agricultural Matrixes and Commercial Formulations by Enzyme-Linked Immunosorbent Assay

2001 ◽  
Vol 84 (4) ◽  
pp. 1001-1010 ◽  
Author(s):  
Kalidindi Hemalatha ◽  
Namburi B K Venugopal ◽  
Namburi B K Venugopal ◽  
Beedu S Rao
Keyword(s):  
Immunosorbent Assay ◽  
Cross Reactivity ◽  
Competitive Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Agricultural Commodities ◽  
Indirect Competitive Elisa ◽  
Capture Assay ◽  
Ic50 Value ◽  
Antibody Capture

Abstract An enzyme-linked immunosorbent assay (ELISA) was developed for azadirachtin (aza), a biopesticide from the neem tree (Azadirachta indica A. Juss). The immunogen was synthesized by epoxidation using the furan ring in the aza molecule. Rabbits were immunized with either bovine serum albumin (BSA)-azadirachtin or ovalbumin (OA)-azadirachtin conjugate. Evaluation of the antisera by antibody capture assay showed that the antibody titer of antisera raised against OA-aza was 1:30 000. An indirect competitive ELISA was developed with BSA-azadirachtin as coating antigen and aza-specific antibodies raised against OA-aza immunogen. The immunoassay showed an inhibitory concentration (IC50) value of 75 ppb, with a range of detection from 0.5 to 1000 ppb for azadirachtin [based on regression analysis, y = 85.87 (−18.89x); r2 = −0.97]. Cross-reactivity of the antibodies with 2 aza- derivatives (22,23-dihydro-23β-methoxy azadirachtin and 3-tigloylazadirachtol) was 33 and 29%, respectively. The indirect competitive ELISA was validated and evaluated by quantitating aza in spiked agricultural commodities and from neem formulations. Azadirachtin was spiked into 5 different agricultural commodities: tomato, brinjal, coffee, tea, and cotton seed at 500 and 1000 ppb and recovered at 62–100%. In samples drawn from 6 lots, the aza content in neem-seed kernels ranged from 0.1 to 0.15%; in commercial neem formulations the content ranged from 200 to 2000 ppm. The method developed may be applied to environmental monitoring of aza and quality assurance studies of aza-based commercial formulations.

scholarly journals Quantitation of Aflatoxin B1-N7-Guanine Adduct in Urine by Enzyme-Linked Immunosorbent Assay Coupled with Immunoaffinity Chromatography

1997 ◽  
Vol 80 (5) ◽  
pp. 1013-1022 ◽  
Author(s):  
Tfflrumurthy Vidyasagar ◽  
Vadapalli Vyjayanthi ◽  
Nayak Sujatiia ◽  
Beedu Sashidhar Rao ◽  
Ramesh Venkataramana Bhat
Keyword(s):  
Immunosorbent Assay ◽  
Aflatoxin B1 ◽  
Polyclonal Antibodies ◽  
Immunoaffinity Chromatography ◽  
Competitive Elisa ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunoaffinity Column ◽  
Indirect Competitive Elisa

Abstract IndiaA specific and sensitive method to quantitate aflatoxin B1-N7-guanine adduct in urine samples by immunoaffinity chromatography coupled with indirect competitive enzyme-linked immunosorbent assay (ELISA) is reported. A novel in vitro method to synthesize an antigen (bovine serum albumin-guanine-aflatoxin B1) and its use to produce polyclonal antibodies specific to the hapten aflatoxin B-N7-guanine are discussed. An indirect competitive ELISA developed to quantitate aflatoxin adduct showed a 50% inhibition at 15.6 pmol aflatoxin B1-N7-guanine (y=66.73+ (-19.8)x, r=-0.997). Interference by aflatoxin B1 was less than 5%, and no interference by guanine, aflatoxin Gι and aflatoxin M1 was observed. An immunoaffinity column was also developed, by using these polyclonal antibodies, for single-step purification of aflatoxin B1-N7-gua-nine. The immunoaffinity column and the indirect competitive ELISA were evaluated and validated by quantitation of aflatoxin B1-N7-guanine in urine from rats dosed with aflatoxin B1 (1 mg/kg body weight). Spiking studies with standard aflatoxin B1-N7-guanine adduct at 2 and 4 μg/mL phosphate- buffered saline gave 96 and 100% recoveries, respectively, for immunoaffinity cleanup column. The method also was tested successfully for quantitating aflatoxin B1-N7-guanine adduct in spiked human urine. Recoveries of the adduct were 79-90%.

Indirect competitive ELISA and colloidal gold-based immunochromatographic strip for amantadine detection in animal-derived foods

2019 ◽  
Vol 11 (15) ◽  
pp. 2027-2032
Author(s):  
Mingfei Pan ◽  
Jingying Yang ◽  
Shijie Li ◽  
Guozhu Wang ◽  
Junping Wang ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Colloidal Gold ◽  
Antiviral Drug ◽  
Competitive Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Strip ◽  
Indirect Competitive Elisa

In this research, an indirect competitive enzyme-linked immunosorbent assay (ELISA) and colloidal gold-based immunochromatographic (GICG) strip were developed for the detection of the antiviral drug amantadine (AM) in animal-derived foods.

scholarly journals Biotinylated single-chain variable fragment-based enzyme-linked immunosorbent assay for glycocholic acid

The Analyst ◽  
2018 ◽  
Vol 143 (9) ◽  
pp. 2057-2065 ◽  
Author(s):  
Xiping Cui ◽  
Natalia Vasylieva ◽  
Ding Shen ◽  
Bogdan Barnych ◽  
Jun Yang ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Competitive Elisa ◽  
Scfv Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Indirect Competitive Elisa ◽  
Glycocholic Acid

An indirect competitive ELISA was developed for GCA detection based on biotinylated scFv antibody.

scholarly journals Unreliable Measurement of Basophil Maximum Leukotriene Release with the Bühlmann CAST 2000 Enzyme-Linked Immunosorbent Assay Kit

2006 ◽  
Vol 13 (3) ◽  
pp. 420-422 ◽  
Author(s):  
S. E. Burastero ◽  
C. Paolucci ◽  
D. Breda ◽  
G. Monasterolo ◽  
R. E. Rossi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Positive Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Results

ABSTRACT The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcεRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

Sign in / Sign up

Export Citation Format

Share Document