In situ measurement of pH in liquid chromatography systems using a colorimetric approach

2019 ◽  
Vol 11 (3) ◽  
pp. 381-386 ◽  
Author(s):  
Gabriel Leme ◽  
Benjamin Madigan ◽  
Joseph Eikens ◽  
David C. Harmes ◽  
Douglas Richardson ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Mobile Phase ◽  
In Situ Measurement ◽  
Peak Widths

In liquid chromatography differences between the pH of an injected sample and the pH of the mobile phase can have a significant impact on retention times, peak widths, and resolution.

In Situ Measurement of the Transversal Dispersion in Ordered and Disordered Two-Dimensional Pillar Beds for Liquid Chromatography

2014 ◽  
Vol 86 (6) ◽  
pp. 2947-2954 ◽  
Author(s):  
Selm De Bruyne ◽  
Wim De Malsche ◽  
Sander Deridder ◽  
Han Gardeniers ◽  
Gert Desmet
Keyword(s):  
Liquid Chromatography ◽  
In Situ Measurement ◽  
Two Dimensional

In Situ Measurement of Soil Heat Flux with the Gradient Method

2003 ◽  
Vol 2 (4) ◽  
pp. 589
Author(s):  
Douglas R. Cobos ◽  
John M. Baker
Keyword(s):  
Heat Flux ◽  
Gradient Method ◽  
Soil Heat Flux ◽  
In Situ Measurement

Analysis of organic impurities of besifloxacin hydrochloride by high performance liquid chromatography with isocratic and gradient elution.

2019 ◽  
Vol 16 ◽  
Author(s):  
Joanna Wittckind Manoel ◽  
Camila Ferrazza Alves Giordani ◽  
Livia Maronesi Bueno ◽  
Sarah Chagas Campanharo ◽  
Elfrides Eva Sherman Schapoval ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Gradient Elution ◽  
Pharmaceutical Product ◽  
Raw Material ◽  
Isocratic Elution ◽  
Organic Impurities ◽  
Elution Method

Introduction: Impurity analysis is an important step in the quality control of pharmaceutical ingredients and final product. Impurities can arise from drug synthesis or excipients and even at small concentrations may affect product efficacy and safety. In this work two methods using high performance liquid chromatography (HPLC) were developed and validated for the evaluation of besifloxacin and its impurity synthesis, with isocratic elution and another with gradient elution. Method: The analysis by HPLC in isocratic elution mode was performed using a cyano column maintained at 25 °C. The mobile phase was composed by 0.5% triethylamine (pH 3.0): acetonitrile (88:12 v/v) eluted at a flow rate of 1.0 ml/min with detection at 330 nm. The gradient elution method was carried out with the same column and mobile phase components only modifying the rate between organic and aqueous phase during analysis. The procedures have been validated according to internationally accepted guidelines, observing results within acceptable limits. Results: The methods presented were found to be linear in the 140 to 260 µg/ml range for besifloxacin and 0.3 to 2.3 µg/ml for an impurity named A. The limits of detection and quantification were respectively 0.07 and 0.3 µg/ml for impurity A, with a 20 µL injection volume. The precision achieved for all analyses performed provided RSD inter-day equal to 6.47 and 6.36% for impurity A with isocratic elution and gradient, respectively. The accuracy was higher than 99% and robustness exhibited satisfactory results. In the isocratic method an analysis time of 25 min and 15 min was obtained for gradient. For impurity A, the number of theoretical plates in the isocratic mode was about 5000 while in the gradient mode it was about 45000, hence, it made the column more efficient by changing the mobile phase composition during elution. In besifloxacin raw material and in pharmaceutical product used in this study, other related impurities were present but but impurity A was searched for and not detected Conclusion: The proposed methods can be applied for quantitative determination of impurities in the analysis of the besifloxacin raw material, as well as in ophthalmic suspension of the drug, considering the quantitation limit.

In-Situ Measurement Technique of Stress and Structure Change

2015 ◽  
Vol 84 (8) ◽  
pp. 567-572
Author(s):  
Tadafumi HASHIMOTO ◽  
Masahito MOCHIZUKI
Keyword(s):  
Measurement Technique ◽  
Structure Change ◽  
In Situ Measurement

Separation and Determination of Some Aromatic Sulfones by Reversed-Phase High-Performance Liquid Chromatography

1994 ◽  
Vol 59 (3) ◽  
pp. 569-574 ◽  
Author(s):  
Josef Královský ◽  
Marta Kalhousová ◽  
Petr Šlosar
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Uv Spectra ◽  
Optimum Conditions ◽  
Linear Calibration ◽  
Technical Grade

The reversed-phase high-performance liquid chromatography of some selected, industrially important aromatic sulfones has been investigated. The chromatographic behaviour of three groups of aromatic sulfones has been studied. The optimum conditions of separation and UV spectra of the sulfones and some of their hydroxy and benzyloxy derivatives are presented. The dependences of capacity factors vs methanol content in mobile phase are mentioned. The results obtained have been applied to the quantitative analysis of different technical-grade samples and isomer mixtures. For all the separation methods mentioned the concentration ranges of linear calibration curves have been determined.

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