A dual signal amplification method for miR-204 assay by combining chimeric molecular beacon with double-stranded nuclease

2018 ◽  
Vol 10 (48) ◽  
pp. 5834-5841 ◽  
Author(s):  
Hao Liu ◽  
Jialong Fan ◽  
Weiping Liu ◽  
Chunyi Tong ◽  
Zhenhua Xie ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Molecular Beacon ◽  
Disease Diagnosis ◽  
Early Disease ◽  
Amplification Method ◽  
Dual Signal Amplification

Due to the miRNA's key roles in early disease diagnosis, an assay based on chimeric molecular beacon was constructed for miR204 detection with LOD lower to 50 pM.

A dual signal amplification method for exosome detection based on DNA dendrimer self-assembly

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 1995-2002 ◽  
Author(s):  
Mei-Ling Gao ◽  
Fang He ◽  
Bin-Cheng Yin ◽  
Bang-Ce Ye
Keyword(s):  
Self Assembly ◽  
Signal Amplification ◽  
Vital Role ◽  
Amplification Method ◽  
Dual Signal Amplification

An increasing number of studies have found that circulating exosomes play a vital role in the occurrence and metastasis of cancer.

A dual-signal amplification method for the DNA detection based on exonuclease III

2014 ◽  
Vol 61 ◽  
pp. 370-373 ◽  
Author(s):  
Zhimin Cai ◽  
Yiying Chen ◽  
Chunshui Lin ◽  
Yanfang Wu ◽  
Chaoyong James Yang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dna Detection ◽  
Exonuclease Iii ◽  
Amplification Method ◽  
Dual Signal Amplification

A sensitive dual signal amplification method for western blotting based on antibody-functionalised graphene oxide and gold nanoparticles

The Analyst ◽  
2012 ◽  
Vol 137 (16) ◽  
pp. 3620 ◽  
Author(s):  
Hongjun Lin ◽  
Jingrui Huo ◽  
Aihong Zhang ◽  
Yingfu Liu ◽  
Qingming Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Graphene Oxide ◽  
Western Blotting ◽  
Signal Amplification ◽  
Amplification Method ◽  
Dual Signal Amplification

scholarly journals Single Step In Situ Detection of Surface Protein and MicroRNA in Clustered Extracellular Vesicles Using Flow Cytometry

2021 ◽  
Vol 10 (2) ◽  
pp. 319
Author(s):  
Hee Cheol Yang ◽  
Won Jong Rhee
Keyword(s):  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Molecular Beacon ◽  
Disease Diagnosis ◽  
Single Step ◽  
Flow Cytometer ◽  
Surface Proteins ◽  
Biomarker Detection ◽  
In Situ Detection

Because cancers are heterogeneous, it is evident that multiplexed detection is required to achieve disease diagnosis with high accuracy and specificity. Extracellular vesicles (EVs) have been a subject of great interest as sources of novel biomarkers for cancer liquid biopsy. However, EVs are nano-sized particles that are difficult to handle; thus, it is necessary to develop a method that enables efficient and straightforward EV biomarker detection. In the present study, we developed a method for single step in situ detection of EV surface proteins and inner miRNAs simultaneously using a flow cytometer. CD63 antibody and molecular beacon-21 were investigated for multiplexed biomarker detection in normal and cancer EVs. A phospholipid-polymer-phospholipid conjugate was introduced to induce clustering of the EVs analyzed using nanoparticle tracking analysis, which enhanced the detection signals. As a result, the method could detect and distinguish cancer cell-derived EVs using a flow cytometer. Thus, single step in situ detection of multiple EV biomarkers using a flow cytometer can be applied as a simple, labor- and time-saving, non-invasive liquid biopsy for the diagnosis of various diseases, including cancer.

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