Highly sensitive and selective detection of miRNA: DNase I-assisted target recycling using DNA probes protected by polydopamine nanospheres

2015 ◽  
Vol 51 (11) ◽  
pp. 2156-2158 ◽  
Author(s):  
Yi Xie ◽  
Xiaoyan Lin ◽  
Yishun Huang ◽  
Rujun Pan ◽  
Zhi Zhu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dnase I ◽  
Dna Probes ◽  
Selective Detection ◽  
Protective Properties ◽  
Target Recycling ◽  
Amplification Method ◽  
Highly Sensitive ◽  
Nuclease Digestion

Based on the protective properties of polydopamine nanospheres for DNA probes against nuclease digestion, we have developed a DNase I-assisted target recycling signal amplification method for highly sensitive and selective detection of miRNA.

Carbon nanoparticle-protected aptamers for highly sensitive and selective detection of biomolecules based on nuclease-assisted target recycling signal amplification

2014 ◽  
Vol 50 (57) ◽  
pp. 7646-7648 ◽  
Author(s):  
Xiaoyan Lin ◽  
Liang Cui ◽  
Yishun Huang ◽  
Ya Lin ◽  
Yi Xie ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Carbon Nanoparticles ◽  
Sensitive Detection ◽  
Selective Detection ◽  
Carbon Nanoparticle ◽  
Target Recycling ◽  
Amplification Method ◽  
Highly Sensitive ◽  
Detection Of Biomolecules ◽  
Highly Sensitive Detection

A nuclease-assisted target recycling signal amplification method based on carbon nanoparticles for highly sensitive detection of biomolecules was developed.

scholarly journals A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramine.

1995 ◽  
Vol 43 (4) ◽  
pp. 347-352 ◽  
Author(s):  
H M Kerstens ◽  
P J Poddighe ◽  
A G Hanselaar
Keyword(s):  
In Situ Hybridization ◽  
Signal Amplification ◽  
Dna Probes ◽  
Bright Field ◽  
Archival Material ◽  
Amplification Method ◽  
Highly Sensitive ◽  
Catalyzed Reporter Deposition

For amplification of in situ hybridization (ISH) signals, we describe a method using catalyzed reporter deposition (CARD). This amplification method is based on the deposition of biotinylated tyramine (BT) at the location of the DNA probe. The BT precipitate can then visualized with fluorochrome- or enzyme-labeled avidin. Both for bright-field ISH (BRISH) and for fluorescence ISH (FISH), the detection limit was highly increased. This method is especially suitable for visualization of very weak ISH signals, such as those obtained by ISH using locus-specific DNA probes. Furthermore, CARD amplification of ISH signals (CARD-ISH) is highly sensitive, rapid, flexible, and easy to implement. Successful application of CARD-ISH with locus-specific DNA probes on histological and cytological samples may improve the determination of structural chromosomal aberrations in archival material.

Highly sensitive fluorescent aptasensor for Salmonella paratyphi A via DNase I-mediated cyclic signal amplification

2015 ◽  
Vol 7 (24) ◽  
pp. 10243-10250 ◽  
Author(s):  
Xing Yan ◽  
Wenkai Li ◽  
Keyi Liu ◽  
Le Deng
Keyword(s):  
Signal Amplification ◽  
Dnase I ◽  
Fluorimetric Determination ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Fluorescent Aptasensor

An elegant aptasensor was developed for dual fluorimetric determination ofSalmonella paratyphi Athrough DNase I-assisted target recycling enlargement.

scholarly journals SARS-CoV-2 RNA Detection with Duplex-Specific Nuclease Signal Amplification

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 197
Author(s):  
Meiqing Liu ◽  
Haoran Li ◽  
Yanwei Jia ◽  
Pui-In Mak ◽  
Rui P. Martins
Keyword(s):  
Signal Amplification ◽  
Incubation Temperature ◽  
Dna Probes ◽  
Detection Sensitivity ◽  
N Gene ◽  
Rna Detection ◽  
Gold Standard Method ◽  
Complementary Method ◽  
Amplification Method ◽  
Virus Rna

The emergence of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a zoonotic pathogen, has led to the outbreak of coronavirus disease 2019 (COVID-19) pandemic and brought serious threats to public health worldwide. The gold standard method for SARS-CoV-2 detection requires both reverse transcription (RT) of the virus RNA to cDNA and then polymerase chain reaction (PCR) for the cDNA amplification, which involves multiple enzymes, multiple reactions and a complicated assay optimization process. Here, we developed a duplex-specific nuclease (DSN)-based signal amplification method for SARS-CoV-2 detection directly from the virus RNA utilizing two specific DNA probes. These specific DNA probes can hybridize to the target RNA at different locations in the nucleocapsid protein gene (N gene) of SARS-CoV-2 to form a DNA/RNA heteroduplex. DSN cleaves the DNA probe to release fluorescence, while leaving the RNA strand intact to be bound to another available probe molecule for further cleavage and fluorescent signal amplification. The optimized DSN amount, incubation temperature and incubation time were investigated in this work. Proof-of-principle SARS-CoV-2 detection was demonstrated with a detection sensitivity of 500 pM virus RNA. This simple, rapid, and direct RNA detection method is expected to provide a complementary method for the detection of viruses mutated at the PCR primer-binding regions for a more precise detection.

An electrochemical aptasensor based on cocoon-like DNA nanostructure signal amplification for the detection of Escherichia coli O157:H7

The Analyst ◽  
2020 ◽  
Vol 145 (22) ◽  
pp. 7340-7348
Author(s):  
Huasong Bai ◽  
Shengjun Bu ◽  
Wensen Liu ◽  
Chengyu Wang ◽  
Zhongyi Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Signal Amplification ◽  
Selective Detection ◽  
Escherichia Coli O157 ◽  
Dna Nanostructures ◽  
Electrochemical Aptasensor ◽  
Dna Nanostructure ◽  
Highly Sensitive

We developed an electrochemical aptasensor based on cocoon-like DNA nanostructures as signal tags for highly sensitive and selective detection of Escherichia coli O157:H7.

Ratiometric fluorescence and mesoporous structure dual signal amplification for sensitive and selective detection of TNT based on MIP@QD fluorescence sensors

2015 ◽  
Vol 51 (15) ◽  
pp. 3200-3203 ◽  
Author(s):  
Shoufang Xu ◽  
Hongzhi Lu
Keyword(s):  
Signal Amplification ◽  
Mesoporous Structure ◽  
Selective Detection ◽  
Ratiometric Fluorescence ◽  
Fluorescence Sensors ◽  
Highly Sensitive ◽  
Silica Materials ◽  
Mesoporous Silica Materials ◽  
Highly Sensitive Detection ◽  
Dual Signal Amplification

MIP@QD fluorescence sensors combined with ratiometric fluorescence techniques and mesoporous silica materials for highly sensitive detection of TNT.

Highly sensitive detection of nucleic acids using a cascade amplification strategy based on exonuclease III-assisted target recycling and conjugated polyelectrolytes

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4267-4272 ◽  
Author(s):  
Biqing Bao ◽  
Yanrui Pan ◽  
Bingbing Gu ◽  
Jia Chen ◽  
Yu Xu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Conjugated Polyelectrolytes ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Exo Iii ◽  
Highly Sensitive Detection ◽  
Amplification Strategy ◽  
Cascade Amplification

A ratiometric and cascade amplification strategy that combines the signal amplification and effecitive FRET property of CPEs with the Exo III-assisted target recycling method has been developed for DNA detection.

A potentiometric aptasensor for carcinoembryonic antigen (CEA) on graphene oxide nanosheets using catalytic recycling of DNase I with signal amplification

2018 ◽  
Vol 10 (45) ◽  
pp. 5364-5371 ◽  
Author(s):  
Zhilin Hong ◽  
Genwang Chen ◽  
Shaoyang Yu ◽  
Rongfu Huang ◽  
Chunmei Fan
Keyword(s):  
Graphene Oxide ◽  
Carcinoembryonic Antigen ◽  
Modified Electrode ◽  
Signal Amplification ◽  
Dnase I ◽  
Label Free ◽  
Graphene Oxide Nanosheets ◽  
Target Recycling

A label-free potentiometric aptasensing platform was designed for detection of carcinoembryonic antigen on a graphene oxide-modified electrode coupling with target recycling-assisted signal amplification.

Sign in / Sign up

Export Citation Format

Share Document