Key factors in FTIR spectroscopic analysis of DNA: the sampling technique, pretreatment temperature and sample concentration

2018 ◽  
Vol 10 (21) ◽  
pp. 2436-2443 ◽  
Author(s):  
Yahong Han ◽  
Lujia Han ◽  
Yumei Yao ◽  
Yanfei Li ◽  
Xian Liu

Fourier transform infrared (FTIR) spectroscopy has been considered as a powerful tool for analysing the characteristics of deoxyribonucleic acid (DNA) regardless of physical states, sample amounts and the molecular weight of DNA.

2015 ◽  
Vol 8 (3) ◽  
pp. 1005-1010 ◽  
Author(s):  
Quinn Burlingame ◽  
Xiaoran Tong ◽  
Jeanne Hankett ◽  
Michael Slootsky ◽  
Zhan Chen ◽  
...  

Using a combination of Fourier transform infrared (FTIR) spectroscopy and physics-based degradation models, we find that the early aging of small molecular weight organic photovoltaic (OPV) cells is due to photochemical degradation of the C60acceptor layer.


2010 ◽  
Vol 129-131 ◽  
pp. 872-876
Author(s):  
Yan Fen Ma ◽  
Sheng Hua Lv ◽  
Gang Liu ◽  
Jian Ping Duan ◽  
Rui Jun Gao

The copolymer of phenol and acrylamide was synthesized by copolymerization using horseradish peroxidase (HRP)/H2O2 as catalyst. The effect of monomer composition on the copolymer tanning agent properties was investigated. The structure of the copolymer was characterized by Fourier transform infrared (FTIR) spectroscopy. The molecular weight was determined by gel permeation chromatography (GPC). The result shows that the average molecular weight of the copolymer is about 1900. Its molecule distribution index is much narrower. The copolymer was used as tanning agent, the result shows that the applied leather is excellent in softness and dyeing.


2019 ◽  
Vol 11 (32) ◽  
pp. 4106-4115
Author(s):  
Keshav Kumar ◽  
Anja Giehl ◽  
Ralf Schweiggert ◽  
Claus-Dieter Patz

The present work introduces a novel classification procedure by combining the multidimensional scaling (MDS) with Fourier transform infrared (FTIR) spectroscopy.


2015 ◽  
Vol 12 (2) ◽  
pp. 13
Author(s):  
Muhamad Faridz Osman ◽  
Karimah Kassim

The coordination complexes of Co(II) and Zn(II) with Schiff bases derived from o-phenylenediamine and substituted 2-hydroxybenzaldehyde were prepared All compounds were characterized by Fourier transform infrared (FTIR) spectroscopy and Nuclear magnetic resonance (NMR) spectroscopy elemental analyzers. They were analyzed using impedance spectroscopy in the frequency range of 100Hz-1 MHz. LI and L2 showed higher conductivity compared to their metal complexes, which had values of 1.3 7 x 10-7 and 6.13 x 10-8 S/cm respectively. 


2021 ◽  
pp. 002580242110109
Author(s):  
Sweety Sharma ◽  
Rito Chophi ◽  
Jaskirandeep Kaur Jossan ◽  
Rajinder Singh

The most important task in a criminal investigation is to detect and identify the recovered biological stains beyond reasonable scientific doubt and preserve the sample for further DNA analysis. In the light of this fact, many presumptive and confirmatory tests are routinely employed in the forensic laboratories to determine the type of body fluid. However, the currently used techniques are specific to one type of body fluid and hence it cannot be utilized to differentiate multiple body fluids. Moreover, these tests consume the samples in due process, and thus it becomes a great limitation especially considering the fact that samples are recovered in minute quantity in forensic cases. Therefore, such limitations necessitate the use of non-destructive techniques that can be applied simultaneously to all types of bodily fluids and allow sample preservation for further analysis. In the current work, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy has been used to circumvent the aforementioned limitations. The important factors which could influence the detection of blood such as the effect of substrates, washing/chemical treatment, ageing, and dilution limits on the analysis of blood have been analysed. In addition, blood discrimination from non-blood substance (biological and non-biological in nature) has also been studied. Chemometric technique that is PCA–LDA has been used to discriminate blood from other body fluids and it resulted in 100% accurate classification. Furthermore, blood and non-blood substances including fake blood have also been classified into separate clusters with a 100% accuracy, sensitivity, and specificity. All-inclusive, this preliminary study substantiates the potential application of ATR-FTIR spectroscopy for the non-destructive identification of blood traces in simulated forensic casework conditions with 0% rate of false classification.


Sign in / Sign up

Export Citation Format

Share Document