Correcting the effect of hematocrit in whole blood coagulation analysis on paper-based lateral flow device

2018 ◽  
Vol 10 (24) ◽  
pp. 2869-2874 ◽  
Author(s):  
Hua Li ◽  
Daewoo Han ◽  
Giovanni M. Pauletti ◽  
Michael A. Hegener ◽  
Andrew J. Steckl
Keyword(s):  
Blood Coagulation ◽  
Whole Blood ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Simple Method ◽  
Lateral Flow Device ◽  
Flow Device

A simple method to correct the effect of hematocrit (Hct) on a paper-based lateral flow assay device that is designed to assess whole blood coagulation is reported.

scholarly journals Correction: Correcting the effect of hematocrit in whole blood coagulation analysis on paper-based lateral flow device

2018 ◽  
Vol 10 (24) ◽  
pp. 2939-2939
Author(s):  
Hua Li ◽  
Daewoo Han ◽  
Giovanni M. Pauletti ◽  
Michael A. Hegener ◽  
Andrew J. Steckl
Keyword(s):  
Blood Coagulation ◽  
Whole Blood ◽  
Lateral Flow ◽  
Lateral Flow Device ◽  
Flow Device

Correction for ‘Correcting the effect of hematocrit in whole blood coagulation analysis on paper-based lateral flow device’ by Hua Li et al., Anal. Methods, 2018, DOI: 10.1039/c8ay00192h.

scholarly journals Engineering a simple lateral flow device for animal blood coagulation monitoring

2018 ◽  
Vol 12 (1) ◽  
pp. 014110 ◽  
Author(s):  
Hua Li ◽  
Daewoo Han ◽  
Giovanni M. Pauletti ◽  
Andrew J. Steckl
Keyword(s):  
Blood Coagulation ◽  
Lateral Flow ◽  
Animal Blood ◽  
Lateral Flow Device ◽  
Coagulation Monitoring ◽  
Flow Device

Blood coagulation screening using a paper-based microfluidic lateral flow device

Lab on a Chip ◽  
2014 ◽  
Vol 14 (20) ◽  
pp. 4035-4041 ◽  
Author(s):  
H. Li ◽  
D. Han ◽  
G. M. Pauletti ◽  
A. J. Steckl
Keyword(s):  
Blood Coagulation ◽  
Lateral Flow ◽  
Lateral Flow Device ◽  
Flow Device ◽  
Fluidic Device ◽  
Coagulation Status ◽  
Visual Indication

A simple paper fluidic device provides a visual indication of blood coagulation status.

Evaluation of rapid test kits for quantification of HT-2 and T-2 toxins in naturally contaminated oats

2013 ◽  
Vol 6 (1) ◽  
pp. 31-41 ◽  
Author(s):  
H.U. Aamot ◽  
I.S. Hofgaard ◽  
G. Brodal ◽  
O. Elen ◽  
B. Holen ◽  
...  
Keyword(s):  
Concentration Ratio ◽  
Rapid Test ◽  
Reference Method ◽  
Lateral Flow ◽  
Cross Reaction ◽  
Cross Reactions ◽  
Lateral Flow Device ◽  
Simultaneous Testing ◽  
Flow Device ◽  
Mycotoxin Analysis

The aim of this study was to evaluate the performance and usefulness of three rapid test kits for analysis of HT-2 and T-2 toxins (HT-2 and T-2), two of the most potent trichothecenes commonly found in European oats. Concentrations of these two toxins combined (HT-2+T-2) were analysed in naturally contaminated oat samples (n=68) using the following test kits: Ridascreen® FAST T-2 Toxin (‘Fast ELISA’), DRAFT Ridascreen® HT-2/T-2 (‘Standard ELISA’, not commercially available), and the lateral flow device ROSA® HT-2-T-2 (‘Rosa LFD’). Mycotoxin analysis by LC-MS/MS was used as a reference method. Rosa LFD offered the best reliability, achieving detection that was stable across toxin levels, whereas detection by both ELISA kits differed significantly among toxin levels (P<0.01). The kits were also evaluated regarding agreement with the reference method (measured as Cohen's kappa) at a HT-2+T-2 concentration of 1000 μg/kg in naturally contaminated oats. Agreement was greatest for Rosa LFD (89.2%), intermediate for Standard ELISA (66.8%), and lowest for Fast ELISA (62.2%). Rosa LFD showed cross-reaction of 100% with both T-2 and HT-2. For the ELISA kits, cross-reactions were 100% with T-2 but below 100% with HT-2. Therefore, to estimate the sum of HT-2 and T-2 in an oat sample, it was necessary to re-calculate the data from both ELISA kits according to the known cross-reaction of each kit with HT-2 and the concentration ratio of HT-2 to T-2 in Norwegian oats. Rosa LFD had the highest correlation with LC-MS/MS (R2=0.94), and the corresponding R2 values for Fast and Standard ELISA were 0.61 and 0.83, respectively. Rosa LFD was well suited for on-site detection. Standard ELISA allows simultaneous testing of several samples that are useful for centralised laboratories.

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