scholarly journals Point‐of‐care diagnosis of invasive aspergillosis in non‐neutropenic patients: Aspergillus Galactomannan Lateral Flow Assay versus Aspergillus ‐specific Lateral Flow Device test in bronchoalveolar lavage

Mycoses ◽  
2019 ◽  
Vol 62 (3) ◽  
pp. 230-236 ◽  
Author(s):  
Jeffrey D. Jenks ◽  
Sanjay R. Mehta ◽  
Randy Taplitz ◽  
Saima Aslam ◽  
Sharon L. Reed ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Invasive Aspergillosis ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Lateral Flow Device ◽  
Flow Device ◽  
Neutropenic Patients ◽  
Device Test

scholarly journals A point-of-care neutrophil elastase activity assay identifies bronchiectasis severity, airway infection and risk of exacerbation

2019 ◽  
Vol 53 (6) ◽  
pp. 1900303 ◽  
Author(s):  
Amelia Shoemark ◽  
Erin Cant ◽  
Luis Carreto ◽  
Alexandria Smith ◽  
Martina Oriano ◽  
...  
Keyword(s):  
Neutrophil Elastase ◽  
Point Of Care ◽  
Severity Index ◽  
Forced Expiratory Volume ◽  
Lateral Flow ◽  
Elastase Activity ◽  
Lateral Flow Device ◽  
Airway Infection ◽  
Independent Validation ◽  
Flow Device

IntroductionNeutrophil elastase activity in sputum can identify patients at high risk of airway infection and exacerbations in bronchiectasis. Application of this biomarker in clinical practice is limited, because no point-of-care test is available. We tested whether a novel semi-quantitative lateral flow device (neutrophil elastase airway test stick – NEATstik®) can stratify bronchiectasis patients according to severity, airway infection and exacerbation risk.MethodsSputum samples from 124 patients with stable bronchiectasis enrolled in the UK and Spain were tested using the NEATstik®, which scores neutrophil elastase concentration from 0 (<8 µg·mL−1 elastase activity) to 10 (maximum detectable neutrophil elastase activity). High neutrophil elastase activity was regarded as a NEATstik® grade >6. Severity of disease, airway infection from sputum culture and exacerbations over the 12 months were recorded. An independent validation was conducted in 50 patients from Milan, Italy.Measurements and main resultsPatients had a median age of 69 years and forced expiratory volume in 1 s (FEV1) 69%. High neutrophil elastase activity was associated with worse bronchiectasis severity using the bronchiectasis severity index (p=0.0007) and FEV1 (p=0.02). A high NEATstik® grade was associated with a significant increase in exacerbation frequency, incident rate ratio 2.75 (95% CI 1.63–4.64, p<0.001). The median time to next exacerbation for patients with a NEATstik® grade >6 was 103 days compared to 278 days. The hazard ratio was 2.59 (95% CI 1.71–3.94, p<0.001). Results were confirmed in the independent validation cohort.ConclusionsA novel lateral flow device provides assessment of neutrophil elastase activity from sputum in minutes and identifies patients at increasing risk of airway infection and future exacerbations.

scholarly journals 1462Bronchoalveolar Lavage Lateral-Flow Device Test for Diagnosing Invasive Pulmonary Aspergillosis in ICU patients: a multicenter study

2014 ◽  
Vol 1 (suppl_1) ◽  
pp. S385-S386 ◽  
Author(s):  
Martin Hoenigl ◽  
Jürgen Prattes ◽  
Susanne Eigl ◽  
Cornelia Lass-Flörl ◽  
Birgit Willinger ◽  
...  
Keyword(s):  
Multicenter Study ◽  
Invasive Pulmonary Aspergillosis ◽  
Lateral Flow ◽  
Pulmonary Aspergillosis ◽  
Icu Patients ◽  
Lateral Flow Device ◽  
Flow Device ◽  
Device Test

scholarly journals Performance of Galactomannan Antigen, Beta-d-Glucan, and Aspergillus-Lateral-Flow Device for the Diagnosis of Invasive Aspergillosis

2016 ◽  
Vol 33 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Gökhan Metan ◽  
Muzaffer Keklik ◽  
Gökçen Dinç ◽  
Çiğdem Pala ◽  
Afra Yıldırım ◽  
...  
Keyword(s):  
Invasive Aspergillosis ◽  
Lateral Flow ◽  
Lateral Flow Device ◽  
Flow Device ◽  
Galactomannan Antigen
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