scholarly journals A novel label-free terbium(iii)-aptamer based aptasensor for ultrasensitive and highly specific detection of acute lymphoma leukemia cells

The Analyst ◽  
2019 ◽  
Vol 144 (12) ◽  
pp. 3843-3852 ◽  
Author(s):  
Siwen Wu ◽  
Nuo Yang ◽  
Liping Zhong ◽  
Yiqun Luo ◽  
Huiling Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mortality Rate ◽  
Acute Leukemia ◽  
Hematopoietic Stem Cells ◽  
Leukemia Cells ◽  
Label Free ◽  
Specific Detection ◽  
Hematopoietic Stem ◽  
High Prevalence

Acute leukemia is a malignant clonal disease of hematopoietic stem cells with a high prevalence and mortality rate.

scholarly journals USP15 Deubiquitinase Safeguards Hematopoiesis and Genome Integrity in Hematopoietic Stem Cells and Leukemia Cells

Cell Reports ◽  
2020 ◽  
Vol 33 (13) ◽  
pp. 108533
Author(s):  
Paul van den Berk ◽  
Cesare Lancini ◽  
Carlos Company ◽  
Michela Serresi ◽  
Maria Pilar Sanchez-Bailon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Leukemia Cells ◽  
Genome Integrity ◽  
Hematopoietic Stem

scholarly journals Microfluidic label-free bioprocessing of human reticulocytes from erythroid culture

Lab on a Chip ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 3445-3460
Author(s):  
Kerwin Kwek Zeming ◽  
Yuko Sato ◽  
Lu Yin ◽  
Nai-Jia Huang ◽  
Lan Hiong Wong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Closed Loop ◽  
Lateral Displacement ◽  
Label Free ◽  
Hematopoietic Stem ◽  
Deterministic Lateral Displacement ◽  
Dean Flow ◽  
Flow Fractionation ◽  
Human Rbcs

Developments in Dean flow fractionation (DFF) and deterministic lateral displacement (DLD) for label-free purification of cultured RBCs from human hematopoietic stem cells. An advancement in sorting and closed-loop manufacturing of viable human RBCs.

scholarly journals Physical characterization of hematopoietic stem cells using multidirectional label-free light scatterings

2016 ◽  
Vol 24 (25) ◽  
pp. 28877 ◽  
Author(s):  
Hesam Shahin ◽  
Manisha Gupta ◽  
Anna Janowska-Wieczorek ◽  
Wojciech Rozmus ◽  
Ying Y. Tsui
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Physical Characterization ◽  
Label Free ◽  
Hematopoietic Stem

Leukemia cells impair normal hematopoiesis and induce functionally loss of hematopoietic stem cells through immune cells and inflammation

2018 ◽  
Vol 65 ◽  
pp. 49-54 ◽  
Author(s):  
Ping Cui ◽  
Yuhua Zhang ◽  
Maoxiang Cui ◽  
Zhihong Li ◽  
Guang Ma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Immune Cells ◽  
Leukemia Cells ◽  
Hematopoietic Stem

Human Hematopoietic Stem Cells and Leukemic Cells Form Cadherin-Catenin Based Junctional Complexes with Mesenchymal Stromal Cells

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 1367-1367 ◽  
Author(s):  
Patrick Wuchter ◽  
Rainer Saffrich ◽  
Wolfgang Wagner ◽  
Frederik Wein ◽  
Mario Stephan Schubert ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hematopoietic Stem Cells ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Leukemia Cells ◽  
Feeder Layer ◽  
Hematopoietic Stem ◽  
Knock Down ◽  
Junctional Complexes

Abstract The interaction between human hematopoietic stem cells (HSC) and their niche plays a key role in regulating maintenance of “stemness” and differentiation. We have demonstrated that a feeder layer of human mesenchymal stromal cells (MSC) can serve as a surrogate model for the niche for human HSC. We could also show, MSC are intimately connected to one another by a novel kind of adhering junction, consisting of villiformto-vermiform cell projections (processus adhaerentes). With this background, we have analyzed the intercellular junctional complexes between HSC and MSC. In comparison, we also studied the cell-cell contacts between leukemia cells (LC) and MSC. MSC were derived from bone marrow aspirates from healthy voluntary donors. HSC were isolated from umbilical cord blood. Leukemia cells that were CD34+ were obtained from bone marrow aspirates from patients suffering from acute myeloid leukemia at the time point of initial diagnosis. After 24–48 hours of co-cultivation, we stained the cellular contacts with a panel of antibodies specific for various components of tight, gap and adherens junctions. Using advanced confocal laser scanning microscopy in combination with deconvolution and volume rendering software, we were able to produce 3D-images of intercellular junctions between HSC/MSC as well as between LC/MSC. To examine the specific function of N-cadherin, we analyzed the effect of siRNA knock down of N-cadherin in MSC upon co-cultures of HSC and MSC. Intercellular connections between HSC and MSC are mainly characterized by podia formation of the HSC linking to the adjacent MSC. At the intimate contact zone to the MSC, we have identified the cytoplasmic plaque proteins alpha- and beta-catenin, co-localized with the transmembrane glycoprotein N-cadherin. Additionally, we compared these findings with a similar setting consisting of human LC co-cultured with feeder-layer of MSC. Our results demonstrated that in comparison to HSC, the proportion of leukemia cells adherent to the feeder-layer is significantly lower and podia formation is less frequent (ratio 1:3). However, the mechanism of adhesion through cadherin-catenin-complex has remained the same. At a functional level, we found that siRNA knock down of N-cadherin in MSC resulted in decreased adhesion of HSC to MSC and in a reduction of cell divisions of HSC. These results confirm that direct cellular contact via N-cadherin-based junctions is essential for homing and adhesion of HSC to the cellular niche and subsequently for the regulation of self-renewal versus differentiation in HSC.

scholarly journals GSK3 Deficiencies in Hematopoietic Stem Cells Initiate Pre-neoplastic State that Is Predictive of Clinical Outcomes of Human Acute Leukemia

Cancer Cell ◽  
2016 ◽  
Vol 29 (1) ◽  
pp. 61-74 ◽  
Author(s):  
Borhane Guezguez ◽  
Mohammed Almakadi ◽  
Yannick D. Benoit ◽  
Zoya Shapovalova ◽  
Susann Rahmig ◽  
...  
Keyword(s):  
Stem Cells ◽  
Acute Leukemia ◽  
Hematopoietic Stem Cells ◽  
Clinical Outcomes ◽  
Hematopoietic Stem

scholarly journals Effect of CTLA4 gene polymorphism on relapse probability among patients with acute leukemias after allogenic hematopoietic stem cells transplantation

2019 ◽  
Vol 14 (1) ◽  
pp. 76-82
Author(s):  
D. S. Romaniuk ◽  
A. A. Khmelevskaya ◽  
M. Yu. Drokov ◽  
N. N. Popova ◽  
V. A. Vasilieva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Acute Leukemia ◽  
Hematopoietic Stem Cells ◽  
Tumor Control ◽  
Unrelated Donor ◽  
Acute Leukemias ◽  
Hematopoietic Stem ◽  
Long Term Outcome ◽  
Free Survival ◽  
Ctla4 Gene

Background.Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is being widely applied as a therapy for hematological malignancies. The long-term outcome of allo-HSCT depends directly on the ability of cytotoxic T-lymphocytes to recognize and eliminate the residual tumor. CTLA-4 is one of the regulatory proteins that provide control over the development of the immune response. Polymorphisms in the CTLA4 gene can affect its function and the efficiency of the antitumor response.The objective:to study the effect of non-synonymous single nucleotide polymorphism (nsSNP) c.49A>G in the donor CTLA4 gene on tumor control in the recipient of allogeneic hematopoietic stem cells (HSC).Materials and methods.Donors of HSC were genotyped for nsSNP c.49A>G in the CTLA4 gene by the real-time polymerase chain reaction using the allele-specific primers. Genotyping data was validated by Sanger’s sequencing of 22 randomly selected samples. The overall survival, the event-free survival and relapse probability were calculated using the Kaplan–Mayer method. A log-rank test was used to assess the statistical significance of group disparities. A p-value of 0.05 was considered as significant.Results.The frequencies of the CTLA4 gene c.49A>G polymorphism alleles in the observed population (102 healthy donors of HSC) correspond to the frequencies obtained by the “1000 genomes” project for the European population. The effect of the donor CTLA4 polymorphism on the tumor control was evaluated on the cohort of patients with acute leukemia after human leukocyte antigen (HLA) matched HSCT from an unrelated donor. It was shown, the three-year relapse-free survival was significantly lower for those patients who received grafts from a donor with the homozygous A/A state of nsSNP c.49A>G (p = 0.01), it was 12.7 % versus 62,8 % in group with c.49A>G G/G and A/G donor genotypes. The incidence of relapse was also significantly different for the group with A/A genotype and for the group with G/G or A/G genotypes of the nsSNP and equaled to 83.7 and 29.3 % respectively (p = 0.03).Conclusion.Patients with acute leukemia, who underwent allo-HSCT from unrelated completely HLA-matched donors with c.49A>G G/G or A/G genotypes have the significantly lower risk of relapse than patients whose donors had the A/A genotype. These results suggest practicability of the nsSNP genotyping for the optimal donor selection.

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