Electrochemical-digital immunosensor with enhanced sensitivity for detecting human salivary glucocorticoid hormone

The Analyst ◽  
2019 ◽  
Vol 144 (4) ◽  
pp. 1448-1457 ◽  
Author(s):  
Muhammad S. Khan ◽  
Ketan Dighe ◽  
Zhen Wang ◽  
Indrajit Srivastava ◽  
Aaron S. Schwartz-Duval ◽  
...  
Keyword(s):  
Point Of Care ◽  
Steroid Hormone ◽  
Human Saliva ◽  
Sensor Chip ◽  
Point Of Care Testing ◽  
Glucocorticoid Hormone ◽  
Enhanced Sensitivity ◽  
Digital Stress ◽  
Potential Use

In this work, an ultra-sensitive electrochemical-digital sensor chip is devised for potential use as a digital stress analyzer for point-of-care testing (POCT) and preventive on-site recording of the hormone ‘cortisol’, a glucocorticoid class of steroid hormone present in the human saliva.

scholarly journals Enhanced Sensing Behavior of Three-Dimensional Microfluidic Paper-Based Analytical Devices (3D-μPADs) with Evaporation-Free Enclosed Channels for Point-of-Care Testing

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 977
Author(s):  
Jaehyung Jeon ◽  
Chanyong Park ◽  
Dinesh Veeran Ponnuvelu ◽  
Sungsu Park
Keyword(s):  
Exposure Time ◽  
Point Of Care ◽  
Three Dimensional ◽  
Point Of Care Testing ◽  
Detection Zone ◽  
Enhanced Sensitivity ◽  
Gray Intensity ◽  
Novel Method ◽  
Insight Into ◽  
Higher Sensitivity

Despite the potential in fabrication of microfluidic paper-based analytical devices (μPADs) for point-of-care testing (POCT) kits, the development of simple, accurate, and rapid devices with higher sensitivity remains challenging. Here, we report a novel method for 3D-μPAD fabrication with enclosed channels using vat photopolymerization to avoid fluid evaporation. In detail, height of the enclosed channels was adjusted from 0.3 to 0.17 mm by varying the UV exposure time from 1 to 4 s for the top barrier, whereas the exposure time for the bottom and side barriers was fixed. As a result, sample flow in the enclosed channels of 3D-μPADs showed lesser wicking speed with very scant evaporation compared to that in the hemi channels in the 3D-μPADs. The stoppage of evaporation in the enclosed channels significantly improved the gray intensity and uniformity in the detection zone of the 3D-μPADs, resulting in as low as 0.3 mM glucose detection. Thus 3D-μPADs with enclosed channels showed enhanced sensitivity compared to the 3D-μPADs with hemi channels when dealing with a small volume sample. Our work provides a new insight into 3D-μPAD design with enclosed channels, which redefines the methodology in 3D printing.

scholarly journals Point-of-Care Testing for Infectious Diseases: Past, Present, and Future

2017 ◽  
Vol 55 (8) ◽  
pp. 2313-2320 ◽  
Author(s):  
Thomas R. Kozel ◽  
Amanda R. Burnham-Marusich
Keyword(s):  
Infectious Diseases ◽  
New Technologies ◽  
Point Of Care ◽  
Low Cost ◽  
Ease Of Use ◽  
Lateral Flow Immunoassay ◽  
Point Of Care Testing ◽  
Resource Limited Settings ◽  
Resource Limited ◽  
Potential Use

ABSTRACT Point-of-care (POC) diagnostics provide rapid actionable information for patient care at the time and site of an encounter with the health care system. The usual platform has been the lateral flow immunoassay. Recently, emerging molecular diagnostics have met requirements for speed, low cost, and ease of use for POC applications. A major driver for POC development is the ability to diagnose infectious diseases at sites with a limited infrastructure. The potential use in both wealthy and resource-limited settings has fueled an intense effort to build on existing technologies and to generate new technologies for the diagnosis of a broad spectrum of infectious diseases.

Magnetic Digital Microfluidics for Point-of-Care Testing: Where Are We Now?

2020 ◽  
Vol 27 ◽  
Author(s):  
Yi Zhang
Keyword(s):  
System Integration ◽  
Diagnostic Tests ◽  
Point Of Care ◽  
Digital Microfluidics ◽  
Controlled Environment ◽  
Point Of Care Testing ◽  
Microfluidic Platforms ◽  
Interface System ◽  
Technical Issues ◽  
Sample System

: Point-of-care (POC) testing decentralizes the diagnostic tests to the sites near the patient. Many POC tests rely microfluidic platforms for sample-to-answer analysis. Compared to other microfluidic systems, magnetic digital microfluidics demonstrate compelling advantages for POC diagnostics. In this review, we have examined the capability of magnetic digital microfluidics-based POC diagnostic platforms. More importantly, we have categorized POC settings into three classes based on “where is the point”, “who to care” and “how to test”, and evaluated the suitability of magnetic digital microfluidics in various POC settings. Furthermore, we have addressed other technical issues associated with POC testing such as controlled environment, sample-system interface, system integration and information connectivity. We hope this review would provide a guideline for the future development of magnetic digital microfluidics-based platforms for POC testing.

Sign in / Sign up

Export Citation Format

Share Document