A signal amplification strategy and sensing application using single gold nanoelectrodes

The Analyst ◽  
2019 ◽  
Vol 144 (1) ◽  
pp. 310-316 ◽  
Author(s):  
Dongmei Wang ◽  
Hongmei Hua ◽  
Haoran Tang ◽  
Cheng Yang ◽  
Wei Chen ◽  
...  
Keyword(s):  
Signal Amplification ◽  
High Sensitivity ◽  
Electrochemical Nanosensor ◽  
Sensing Application ◽  
Amplification Strategy ◽  
Gold Nanoelectrode

An electrochemical nanosensor was fabricated on a single gold nanoelectrode for thrombin sensing with high sensitivity via a signal amplification strategy.

A QCM Immunosensor for Rapid Determination of Ractopamine in Food Based on Enzyme-AuNPs for Signal Amplification and Magnetic β-Cyclodextrins for Extraction

2015 ◽  
Vol 738-739 ◽  
pp. 56-60
Author(s):  
Shu Xian Chen ◽  
Jing Liu ◽  
Dao Dong Pan ◽  
Ning Gan
Keyword(s):  
Signal Amplification ◽  
Rapid Determination ◽  
Screening Method ◽  
High Sensitivity ◽  
The Novel ◽  
Highly Sensitive ◽  
Reliable Quantification ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

An enzyme amplified immunosensor for highly sensitive detection of Ractopamine (RAC) in foodstuff was developed based on quartz crystal microbalance (QCM). The high sensitivity was achieved by enzyme-AuNPs signal amplification strategy and magnetic β-cyclodextrins (β-CD) enrichment capacity. The novel QCM immunosensor which combines with the advantages of high selectivity of immunoassays and the high sensitivity of QCM has been developed for the determination of trace residues of RAC in food production. Under optimum conditions, the differences in the frequencies (∆f) of the QCM were proportional to the concentration of RAC over the range from 0.01 to 10 ng mL-1. The minimal detection limit was 0.01 ng mL-1. Due to its high sensitivity, acceptable stability and good selectivity, the immunosensor realized reliable quantification of RAC in real foodstuff. The proposed project has the potential to become a successful on-site screening method in food safety.

Dual signal amplification strategy for high-sensitivity detection of copper species in bio-samples with a tunable dynamic range

2018 ◽  
Vol 54 (20) ◽  
pp. 2542-2545 ◽  
Author(s):  
Yamin Xiong ◽  
Peijun Meng ◽  
Huiling Li ◽  
Yue Hu ◽  
Lihong Zhou ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dynamic Range ◽  
High Sensitivity ◽  
Copper Species ◽  
Amplification Strategy ◽  
Dual Signal Amplification ◽  
High Sensitivity Detection ◽  
Sensitivity Detection

A facile method was constructed and applied to detection of copper species in bio-samples with a tunable dynamic range.

An ultrasensitive colorimetric aptasensor for ATP based on peptide/Au nanocomposites and hemin–G-quadruplex DNAzyme

RSC Advances ◽  
2014 ◽  
Vol 4 (44) ◽  
pp. 23185-23190 ◽  
Author(s):  
Shipeng Li ◽  
Liqiang Wang ◽  
Yuanqiang Hao ◽  
Lili Zhang ◽  
Binbin Zhou ◽  
...  
Keyword(s):  
Signal Amplification ◽  
High Sensitivity ◽  
G Quadruplex ◽  
Self Assembled ◽  
Amplification Strategy ◽  
Colorimetric Aptasensor ◽  
Atp Detection

A self-assembled peptide nanosphere was firstly applied to construct biosensors. A new signal amplification strategy was proposed for colorimetric aptasensor based on PNS/AuNPs composite. The colorimetric aptasensor displayed an ultra-high sensitivity for ATP detection with a LOD of 1.35 pM.

scholarly journals Aptameric Molecular Switch for Cascade Signal Amplification

2012 ◽  
Vol 58 (2) ◽  
pp. 384-390 ◽  
Author(s):  
Cuiping Ma ◽  
Chunhui Zhao ◽  
Yujie Ge ◽  
Chao Shi
Keyword(s):  
Human Serum ◽  
Signal Amplification ◽  
Homogeneous Solution ◽  
Protein Detection ◽  
High Sensitivity ◽  
Molecular Switches ◽  
Clinical Applications ◽  
Serum Samples ◽  
Amplification Strategy ◽  
Trace Concentrations

Abstract BACKGROUND High sensitivity of analysis is constantly in demand in biomedical research and clinical diagnosis. In recent years aptamer-based analytical methods have been developed for protein detection. We developed a cascade signal amplification strategy based on molecular switches and aptamers to improve protein detection. METHODS Our cascade signal amplification strategy based on molecular switches and aptamers consisted of 2 steps, including the recognition and the triggering of a polymerase reaction. The procedure was designed to simplify the analysis by detecting trace amounts of target isothermally, in real time, and in a homogeneous solution. We applied this method to measure thrombin in human serum samples. RESULTS This cascade signal amplification strategy exhibited a linear response in thrombin concentration from 0.3 to 10 nmol/L, with a detection limit of 1.7 × 10−10 mol/L within 60 min. Results of the analysis of thrombin in human serum diluted 1:1 appeared to be linear, as was observed in buffer, in the tested concentration range of 0.3–10 nmol/L. CONCLUSIONS The aptameric sensor provides promising potential for detecting and screening trace concentrations of biomarkers in complex matrices for clinical applications.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

Naked-Eye Detection of Hg(II) Ions by Visible Light-Induced Polymerization Initiated by a Hg(II)-Selective Photoredox Catalyst

2021 ◽  
Author(s):  
Hyungwook Kim ◽  
Young Jae Jung ◽  
Jungkyu K. Lee
Keyword(s):  
Visible Light ◽  
Signal Amplification ◽  
Eye Detection ◽  
Naked Eye ◽  
Turn On ◽  
Novel Strategy ◽  
Amplification Strategy ◽  
Naked Eye Detection

We developed a novel strategy for signal amplification strategy using a visible light-induced photopolymerization, initiated by a selective turn-on photoredox catalyst. As photoredox catalysts, fluorescein derivatives are able to initiate...

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