A multiplex paper-based nanobiocatalytic system for simultaneous determination of glucose and uric acid in whole blood

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4422-4428 ◽  
Author(s):  
Jin Huang ◽  
Xue-Li Zhu ◽  
Yu-Min Wang ◽  
Jian-Hui Ge ◽  
Jin-Wen Liu ◽  
...  
Keyword(s):  
Uric Acid ◽  
Simultaneous Determination ◽  
Whole Blood ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
Multiple Targets ◽  
Point Of Care Assay

In this work, a versatile point-of-care assay platform based on a microfluidic paper-based analytic device (μPAD) was developed for the simultaneous detection of multiple targets.

Validation of a new point-of-care assay for determination of β-carotene concentration in bovine whole blood and plasma

2012 ◽  
pp. n/a-n/a ◽  
Author(s):  
Jens Raila ◽  
Francis Enjalbert ◽  
Ralf Mothes ◽  
Andrea Hurtienne ◽  
Florian J. Schweigert
Keyword(s):  
Whole Blood ◽  
Point Of Care ◽  
Β Carotene ◽  
Point Of Care Assay

scholarly journals Carbon Black-Carbon Nanotube Co-Doped Polyimide Sensors for Simultaneous Determination of Ascorbic Acid, Uric Acid, and Dopamine

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1691 ◽  
Author(s):  
Yue Wang ◽  
Tian Yang ◽  
Yasushi Hasebe ◽  
Zhiqiang Zhang ◽  
Dongping Tao
Keyword(s):  
Ascorbic Acid ◽  
Uric Acid ◽  
Carbon Nanotube ◽  
Carbon Black ◽  
Simultaneous Determination ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Differential Pulse ◽  
Co Doped

Carbon black (CB) and carbon nanotube (CNT) co-doped polyimide (PI) modified glassy carbon electrode (CB-CNT/PI/GCE) was first prepared for the simultaneous determination of ascorbic acid (AA), dopamine (DA), and uric acid (UA). The CB-CNT/PI/GCE exhibited persistent electrochemical behavior and excellent catalytic activities. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used for the simultaneous detection of AA, DA, and UA in their ternary mixture. The peak separations between AA and DA, and DA and UA, are up to 166 mV and 148 mV, respectively. The CB-CNT/PI/GCE exhibited high sensitivity to DA and UA, with the detection limit of 1.9 µM and 3 µM, respectively. In addition, the CB-CNT/PI/GCE showed sufficient selectivity and long-term stability, and was applicable to detect AA, DA, and UA in human urine sample.

A cost-effective disposable graphene-modified electrode decorated with alternating layers of Au NPs for the simultaneous detection of dopamine and uric acid in human urine

RSC Advances ◽  
2016 ◽  
Vol 6 (84) ◽  
pp. 80756-80765 ◽  
Author(s):  
Nadeem Baig ◽  
Abdel-Nasser Kawde
Keyword(s):  
Uric Acid ◽  
Simultaneous Determination ◽  
Human Urine ◽  
Simultaneous Detection ◽  
Cost Effective ◽  
Au Nps ◽  
Graphene Layers ◽  
Disposable Electrode ◽  
Highly Sensitive

A disposable electrode based on a highly sensitive and readily fabricated arrangement of alternating AuNP and graphene layers was introduced for the simultaneous determination of dopamine and uric acid.

scholarly journals Simultaneous Determination of Uric Acid and Xanthine Using a Poly(Methylene Blue) and Electrochemically Reduced Graphene Oxide Composite Film Modified Electrode

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Gen Liu ◽  
Wei Ma ◽  
Yan Luo ◽  
Deng-ming Sun ◽  
Shuang Shao
Keyword(s):  
Methylene Blue ◽  
Uric Acid ◽  
Graphene Oxide ◽  
Composite Film ◽  
Simultaneous Determination ◽  
Reduced Graphene Oxide ◽  
Modified Electrode ◽  
Reduced Graphene ◽  
Electrochemically Reduced Graphene Oxide

Poly(methylene blue) and electrochemically reduced graphene oxide composite film modified electrode (PMB-ERGO/GCE) was successfully fabricated by electropolymerization and was used for simultaneous determination of uric acid (UA) and xanthine (Xa). Based on the excellent electrocatalytic activity of PMB-ERGO/GCE, the electrochemical behaviors of UA and Xa were studied by cyclic voltammetry (CV) and square wave voltammetry (SWV). Two anodic sensitive peaks at 0.630 V (versus Ag/AgCl) for UA and 1.006 V (versus Ag/AgCl) for Xa were given by CV in pH 3.0 phosphate buffer. The calibration curves for UA and Xa were obtained in the range of 8.00 × 10−8~4.00 × 10−4 M and 1.00 × 10−7~4.00 × 10−4 M, respectively, by SWV. The detection limits for UA and Xa were3.00×10-8 M and5.00×10-8 M, respectively. Finally, the proposed method was applied to simultaneously determine UA and Xa in human urine with good selectivity and high sensitivity.

scholarly journals MnO2@Reduced Graphene Oxide Nanocomposite-Based Electrochemical Sensor for the Simultaneous Determination of Trace Cd(II), Zn(II) and Cu(II) in Water Samples

Membranes ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 517
Author(s):  
Siyamthanda Hope Mnyipika ◽  
Tshimangadzo Saddam Munonde ◽  
Philiswa Nosizo Nomngongo
Keyword(s):  
Heavy Metals ◽  
Electrochemical Sensor ◽  
Simultaneous Determination ◽  
Rapid Detection ◽  
Electrochemical Sensors ◽  
Simultaneous Detection ◽  
Cyclic Voltammograms ◽  
Current Response ◽  
Mno2 Nanoparticles

The rapid detection of trace metals is one of the most important aspect in achieving environmental monitoring and protection. Electrochemical sensors remain a key solution for rapid detection of heavy metals in environmental water matrices. This paper reports the fabrication of an electrochemical sensor obtained by the simultaneous electrodeposition of MnO2 nanoparticles and RGO nanosheets on the surface of a glassy carbon electrode. The successful electrodeposition was confirmed by the enhanced current response on the cyclic voltammograms. The XRD, HR-SEM/EDX, TEM, FTIR, and BET characterization confirmed the successful synthesis of MnO2 nanoparticles, RGO nanosheets, and MnO2@RGO nanocomposite. The electrochemical studies results revealed that MnO2@RGO@GCE nanocomposite considerably improved the current response on the detection of Zn(II), Cd(II) and Cu(II) ions in surface water. These remarkable improvements were due to the interaction between MnO2 nanomaterials and RGO nanosheets. Moreover, the modified sensor electrode portrayed high sensitivity, reproducibility, and stability on the simultaneous determination of Zn(II), Cd(II), and Cu(II) ions. The detection limits of (S/N = 3) ranged from 0.002–0.015 μg L−1 for the simultaneous detection of Zn(II), Cd(II), and Cu(II) ions. The results show that MnO2@RGO nanocomposite can be successfully used for the early detection of heavy metals with higher sensitivity in water sample analysis.

scholarly journals Development of a multicolor upconversion fluorescence immunoassay for the simultaneous detection of thiamethoxam and dextran by magnetic separation

RSC Advances ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 517-524
Author(s):  
Chuanyong Li ◽  
Wanlin Sun ◽  
Lianrun Huang ◽  
Nana Sun ◽  
Xiude Hua ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Simultaneous Determination ◽  
Magnetic Separation ◽  
Simultaneous Detection ◽  
Fluorescence Immunoassay ◽  
Upconversion Fluorescence

The anti-thiamethoxam and anti-dextran monoclonal antibodies were prepared to develop a multicolor upconversion fluorescence immunoassay for the simultaneous determination of thiamethoxam (544 nm) and dextran (477 nm).

scholarly journals Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Mustafa Karapirli ◽  
Murat Kizilgun ◽  
Ozgur Yesilyurt ◽  
Husamettin Gul ◽  
Zeki Ilker Kunak ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Simultaneous Determination ◽  
Cyclosporine A ◽  
Whole Blood ◽  
Immunosuppressive Drugs ◽  
Tandem Mass ◽  
Blood Samples ◽  
Whole Blood Samples

Objectives. Cyclosporine A (CyA), tacrolimus (TRL), sirolimus (SIR), and everolimus (RAD) are immunosuppressive drugs frequently used in organ transplantation. Our aim was to confirm a robust sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination of CyA, TRL, SIR, and RAD in whole-blood samples.Materials and Methods. We used an integrated online solid-phase extraction-LC-MS/MS system and atmospheric pressure ionization tandem mass spectrometry (API-MS/MS) in the multiple reaction monitoring (MRM) detection mode. CyA, TRL, SIR, and RAD were simultaneously analyzed in whole blood treated with precipitation reagent taken from transplant patients.Results. System performance parameters were suitable for using this method as a high-throughput technique in clinical practice. The high concentration of one analyte in the sample did not affect the concentration of other analytes. Total analytical time was 2.5 min, and retention times of all analytes were shorter than 2 minutes.Conclusion. This LC-MS/MS method can be preferable for therapeutic drug monitoring of these immunosuppressive drugs (CyA, TRL, SRL, and RAD) in whole blood. Sample preparation was too short and simple in this method, and it permits robust, rapid, sensitive, selective, and simultaneous determination of these drugs.

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