scholarly journals First characterization of a biphasic, switch-like DNA amplification

The Analyst ◽  
2018 ◽  
Vol 143 (8) ◽  
pp. 1820-1828 ◽  
Author(s):  
Burcu Özay ◽  
Cara M. Robertus ◽  
Jackson L. Negri ◽  
Stephanie E. McCalla
Keyword(s):  
Dna Amplification ◽  
High Gain

An isothermal, high-gain DNA amplification chemistry with biphasic and switch-like properties.

scholarly journals Characterization of a high-gain Ne-like Fe transient x-ray laser

1999 ◽  
Author(s):  
Stephen J. Moon ◽  
James Dunn ◽  
Anatoly Y. Faenov ◽  
Tatiana A. Pikuz ◽  
Kevin B. Fournier ◽  
...  
Keyword(s):  
High Gain ◽  
X Ray

scholarly journals Morphological and molecular characterization of Strongyloides ophidiae (Nematoda, Strongyloididae)

2009 ◽  
Vol 84 (2) ◽  
pp. 136-142 ◽  
Author(s):  
K.R. dos Santos ◽  
B.C. Carlos ◽  
K.S. Paduan ◽  
S.M. Kadri ◽  
T.H. Barrella ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Dna Amplification ◽  
Strongyloides Stercoralis ◽  
Morphological Data ◽  
Nucleotide Sequence Analysis ◽  
Parasitic Female ◽  
Strongyloides Venezuelensis ◽  
Polymerase Chain ◽  
Morphological And Molecular Characterization

AbstractThe aim of the present study is to report morphological data from parasitic female, rhabditoid and filarioid larvae, free-living female worms and eggs of Strongyloides ophidiae (Nematoda, Strongyloididae). In addition, a molecular DNA analysis was carried out using a pool of eight S. ophidiae parasitic females. Samples were obtained from the small intestine of Oxyrhopus guibei (Serpentes, Colubridae) collected in the municipality of Lençóis Paulista, State of São Paulo, Brazil. DNA amplification by polymerase chain reaction (PCR) resulted in a 350 bp band for samples containing S. ophidiae and Strongyloides venezuelensis DNA. Strongyloides ophidiae nucleotide sequence analysis showed 98% similarity with Strongyloides procyonis and 97% with Strongyloides cebus, Strongyloides stercoralis, Strongyloides fuelleborni and Strongyloides sp. from snakes.

Fabrication and Characterization of ZnO Photodetectors with High Gain

2010 ◽  
Vol 4 (3) ◽  
pp. 316-320 ◽  
Author(s):  
Ghusoon M. Ali ◽  
S. Singh ◽  
P. Chakrabarti
Keyword(s):  
High Gain ◽  
Fabrication And Characterization

A YAC contig map of Plasmodium falciparum chromosome 4: characterization of a DNA amplification between two recently separated isolates

Genomics ◽  
1995 ◽  
Vol 26 (2) ◽  
pp. 192-198 ◽  
Author(s):  
Justin P. Rubio ◽  
Tony Triglia ◽  
David J. Kemp ◽  
Derik de Bruin ◽  
Jeffrey V. Ravetch ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Dna Amplification ◽  
Chromosome 4 ◽  
Yac Contig ◽  
Contig Map

scholarly journals 678 PB 186 RAPD GENETIC MARKERS FOR CHARACTERIZATION OF MUSA GENETIC RESOURCES

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 530a-530
Author(s):  
R.L. Jarret ◽  
K.V. Bhat
Keyword(s):  
Genetic Markers ◽  
Size Range ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Dna Amplification ◽  
Principal Coordinate Analysis ◽  
Phenetic Analysis ◽  
Similarity Coefficients ◽  
Primer Sequence

Fifty-seven accessions of Musa including cultivated clones Of 6 genomic groups (AA, AB, AAA, AAB, ABB, ABBB), M. balbisiana (BB), M. acuminata ssp. banksii (AA), M. acuminata ssp. malaccensis (AA) and M. velutina were examined for random amplified polymorphic DNA (RAPD) genetic markers using PCR with sixty 10-mer random primers. Forty-nine of 60 tested primers gave reproducible DNA amplification patterns. The number of bands resolved per amplification was primer dependent and varied from 1 to a maximum of 24. The size range of the amolification products also differed with the select& primer sequence/genotype and ranged from 0.29 to 3.0 kb. RAPD data were used to generate Jaccard's similarity coefficients which were analyzed phenetically. Phenetic analysis separated clones into distinct groupings that were in agreement with clusterings revealed when data were subsequently analyzed by principal coordinate analysis (PCO). In both the phenetic and the PCO analyses, previously unclassified cultivars grouped with cultivars previously classified for their genomic group based on morphological keys. The implications of RAPD analysis for Musa germplasm classification, clonal identification, and management are discussed.

scholarly journals First Characterization of a Biphasic, Switch-like DNA Amplification

2017 ◽  
Author(s):  
Burcu Özay ◽  
Cara M Robertus ◽  
Jackson L Negri ◽  
Stephanie E McCalla
Keyword(s):  
Dna Amplification ◽  
High Gain ◽  
Clinical Diagnostics ◽  
Second Phase ◽  
Binding Domains ◽  
Trigger Loop ◽  
Reaction Acceleration ◽  
Biphasic Reaction ◽  
Dna Template ◽  
Amplification Reaction

ABSTRACTWe report the first DNA amplification chemistry with switch-like characteristics: the chemistry is biphasic, with an expected initial phase followed by an unprecedented high gain burst of product oligonucleotide in a second phase. The first and second phases are separated by a temporary plateau, with the second phase producing 10 to 100 times more product than the first. The reaction is initiated when an oligonucleotide binds and opens a palindromic looped DNA template with two binding domains. Upon loop opening, the oligonucleotide trigger is rapidly amplified through cyclic extension and nicking of the bound trigger. Loop opening and DNA association drive the amplification reaction, such that reaction acceleration in the second phase is correlated with DNA association thermodynamics. Without a palindromic sequence, the chemistry resembles the exponential amplification reaction (EXPAR). EXPAR terminates at the initial plateau, revealing a previously unknown phenomenon that causes early reaction cessation in this popular oligonucleotide amplification reaction. Here we present two distinct types of this biphasic reaction chemistry and propose dominant reaction pathways for each type based on thermodynamic arguments. These reactions create an endogenous switch-like output that reacts to approximately 1pM oligonucleotide trigger. The chemistry is isothermal and can be adapted to respond to a broad range of input target molecules such as proteins, genomic bacterial DNA, viral DNA, and microRNA. This rapid DNA amplification reaction could potentially impact a variety of disciplines such as synthetic biology, biosensors, DNA computing, and clinical diagnostics.

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