scholarly journals Comparative effects of a candidate modified-risk tobacco product Aerosol and cigarette smoke on human organotypic small airway cultures: a systems toxicology approach

2017 ◽  
Vol 6 (6) ◽  
pp. 930-946 ◽  
Author(s):  
Anita R. Iskandar ◽  
Yannick Martinez ◽  
Florian Martin ◽  
Walter K. Schlage ◽  
Patrice Leroy ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Heating System ◽  
Tobacco Product ◽  
Small Airway ◽  
Biological Impact ◽  
Systems Toxicology

The biological impact of a tobacco heating system 2.2 aerosol and cigarette smoke were compared.

scholarly journals Systems toxicology meta-analysis of in vitro assessment studies: biological impact of a candidate modified-risk tobacco product aerosol compared with cigarette smoke on human organotypic cultures of the aerodigestive tract

2017 ◽  
Vol 6 (5) ◽  
pp. 631-653 ◽  
Author(s):  
A. R. Iskandar ◽  
B. Titz ◽  
A. Sewer ◽  
P. Leroy ◽  
T. Schneider ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Meta Analysis ◽  
Tobacco Product ◽  
Aerodigestive Tract ◽  
Organotypic Cultures ◽  
Biological Impact ◽  
Systems Toxicology ◽  
In Vitro Assessment

Reduced impact of a tobacco product was observed on the smoking “field-of-injury” tissues.

Robustness of HPHC Reduction in THS 2.2 Aerosol Relative to 3R4F Reference Cigarette Smoke under Extreme Climatic Conditions

2021 ◽  
Vol 30 (3) ◽  
pp. 109-126
Author(s):  
Laurent Poget ◽  
Catherine Goujon ◽  
Samuel Kleinhans ◽  
Serge Maeder ◽  
Jean-Pierre Schaller
Keyword(s):  
Cigarette Smoke ◽  
Heating System ◽  
Tobacco Product ◽  
Climatic Conditions ◽  
Mainstream Smoke ◽  
Humid Climate ◽  
Experimental Conditions ◽  
Puff Volume ◽  
Temperature And Humidity ◽  
Health Canada

Summary In order to assess robustness for the reduction of harmful and potentially harmful constituent (HPHC) levels generated by the Tobacco Heating System 2.2 (THS 2.2), a heated tobacco product, we compared the aerosol of this product with mainstream smoke from the 3R4F reference cigarette under different conditions of temperature and humidity. The desired climatic conditions were achieved by using an air-conditioning system coupled with the smoking-machine housing. Two extreme climatic conditions were selected, representing a “Hot and Dry” climate (30 °C and 35% relative humidity RH) and a “Hot and Very Humid” climate (30 °C and 75% RH). In addition, aerosol and smoke were generated using the standard conditions recognized for smoking-machine analyses of tobacco products (22 °C and 60% RH), which were close to the climatic conditions defined for “Subtropical and Mediterranean” environments (25 °C and 60% RH). The experimental conditions were chosen to simulate the use of THS 2.2 and cigarettes under extreme conditions of temperature and humidity. HeatSticks and cigarettes taken from freshly opened packs were subjected to short-term conditioning from two to a few more days under the same experimental conditions. We analyzed 54 HPHCs in THS 2.2 aerosol and 3R4F cigarette smoke, generated in accordance with the Health Canada Intense (HCI) standard, using modified temperature and humidity conditions for sample conditioning and machine-smoking experiments. We used a volume-adjusted approach for comparing HPHC reductions across the different climatic conditions investigated. Although a single puffing regimen was used, the total puff volume recorded for the 3R4F cigarette smoke varied due to the influence of temperature and humidity on combustion rate, which justified the use of a volume-adjusted approach. Volume-adjusted yields were derived from HPHC yields expressed in mass-per-tobacco stick normalized per total puff volume. The results indicated that, regardless of the considered climatic conditions, the HPHC levels investigated in THS 2.2 aerosol were reduced by at least 90%, on average, when compared with the concentrations in 3R4F cigarette mainstream smoke. This confirmed the robustness in performance for THS 2.2 to deliver reduced levels of HPHCs under the extreme climatic conditions investigated in this study. In order to further characterize the robustness of these reductions, the lowest reduction performance achieved for individual HPHCs across all climatic conditions was used to define the threshold for a robust reduction. The majority of the 54 HPHCs investigated in THS 2.2 aerosol showed more than 90% reduction. Calculations derived from nicotine-adjusted yields also confirmed robust reductions for all investigated HPHCs. The small differences in absolute reduction between the volume- and nicotine-adjusted approaches were predominantly attributed to a combination of the differences in both nominal nicotine deliveries and total puff volumes between THS 2.2 and 3R4F cigarettes; however, this did not influence the determination of robustness. Our findings confirm the value of this approach for assessing the robustness of a product’s performance under different climatic conditions.

scholarly journals Respiratory Effects of Exposure to Aerosol From the Candidate Modified-Risk Tobacco Product THS 2.2 in an 18-Month Systems Toxicology Study With A/J Mice

2020 ◽  
Vol 178 (1) ◽  
pp. 138-158
Author(s):  
Bjoern Titz ◽  
Alain Sewer ◽  
Karsta Luettich ◽  
Ee Tsin Wong ◽  
Emmanuel Guedj ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Stress Responses ◽  
Respiratory Health ◽  
Heating System ◽  
Tobacco Product ◽  
Effective Measure ◽  
Systems Toxicology ◽  
Toxicology Study ◽  
Integrative Assessment ◽  
Toxicology Assessment

Abstract Smoking cessation is the most effective measure for reducing the risk of smoking-related diseases. However, switching to less harmful products (modified-risk tobacco products [MRTP]) can be an alternative to help reduce the risk for adult smokers who would otherwise continue to smoke. In an 18-month chronic carcinogenicity/toxicity study in A/J mice (OECD Test Guideline 453), we assessed the aerosol of Tobacco Heating System 2.2 (THS 2.2), a candidate MRTP based on the heat-not-burn principle, compared with 3R4F cigarette smoke (CS). To capture toxicity- and disease-relevant mechanisms, we complemented standard toxicology endpoints with in-depth systems toxicology analyses. In this part of our publication series, we report on integrative assessment of the apical and molecular exposure effects on the respiratory tract (nose, larynx, and lungs). Across the respiratory tract, we found changes in inflammatory response following 3R4F CS exposure (eg, antimicrobial peptide response in the nose), with both shared and distinct oxidative and xenobiotic responses. Compared with 3R4F CS, THS 2.2 aerosol exerted far fewer effects on respiratory tract histology, including adaptive tissue changes in nasal and laryngeal epithelium and inflammation and emphysematous changes in the lungs. Integrative analysis of molecular changes confirmed the substantially lower impact of THS 2.2 aerosol than 3R4F CS on toxicologically and disease-relevant molecular processes such as inflammation, oxidative stress responses, and xenobiotic metabolism. In summary, this work exemplifies how apical and molecular endpoints can be combined effectively for toxicology assessment and further supports findings on the reduced respiratory health risks of THS 2.2 aerosol.

scholarly journals Systems Toxicology Assessment of the Biological Impact of a Candidate Modified Risk Tobacco Product on Human Organotypic Oral Epithelial Cultures

2016 ◽  
Vol 29 (8) ◽  
pp. 1252-1269 ◽  
Author(s):  
Filippo Zanetti ◽  
Alain Sewer ◽  
Carole Mathis ◽  
Anita R. Iskandar ◽  
Radina Kostadinova ◽  
...  
Keyword(s):  
Tobacco Product ◽  
Biological Impact ◽  
Systems Toxicology ◽  
Epithelial Cultures ◽  
Toxicology Assessment ◽  
Oral Epithelial
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