β-Ni(OH)2 nanosheets: an effective sensing platform for constructing nucleic acid-based optical sensors

2017 ◽  
Vol 5 (35) ◽  
pp. 7426-7432 ◽  
Author(s):  
Hui Zhou ◽  
Junbin Peng ◽  
Xinlan Qiu ◽  
Yansha Gao ◽  
Limin Lu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Optical Sensors ◽  
Short Oligonucleotide ◽  
Sensing Platform ◽  
First Time ◽  
Differential Affinity

We report for the first time that β-Ni(OH)2 nanosheets can exhibit differential affinity toward short oligonucleotide fragment versus ssDNA probe and the absorbed DNA can also be desorbed by degrading the β-Ni(OH)2 nanosheets.

Cobalt disulfide nanowires as an effective fluorescent sensing platform for DNA detection

2016 ◽  
Vol 4 (17) ◽  
pp. 2860-2863 ◽  
Author(s):  
Zhicai Xing ◽  
Lei Wang ◽  
Xiurong Yang
Keyword(s):  
Nucleic Acid ◽  
Hydrothermal Method ◽  
Dna Detection ◽  
Nucleic Acid Detection ◽  
Fluorescent Sensing ◽  
Sensing Platform ◽  
Cobalt Disulfide ◽  
First Time

Cobalt disulfide nanowires are synthesized in solution using a facile two-step hydrothermal method for the first time and applied as an effective sensing platform for nucleic acid detection.

Nanoporous molybdenum carbide nanowires: a novel sensing platform for DNA detection

2015 ◽  
Vol 3 (36) ◽  
pp. 7173-7176 ◽  
Author(s):  
Zhicai Xing ◽  
Lei Wang ◽  
Xuping Sun ◽  
Yuquan He ◽  
Abdullah M. Asiri
Keyword(s):  
Nucleic Acid ◽  
Molybdenum Carbide ◽  
Dna Detection ◽  
Nucleic Acid Detection ◽  
Sensing Platform ◽  
First Time

Nanoporous Mo2C nanowires have been successfully demonstrated as an effective sensing platform for nucleic acid detection for the first time.

Optical Sensors for Detection of Amino Acids

2018 ◽  
Vol 25 (19) ◽  
pp. 2272-2290 ◽  
Author(s):  
Aafrin M. Pettiwala ◽  
Prabhat K. Singh
Keyword(s):  
Amino Acids ◽  
Optical Sensors ◽  
Supramolecular Assemblies ◽  
Significant Activity ◽  
Physiological Level ◽  
Biological Scaffolds ◽  
Detection Systems ◽  
Sensing Platform ◽  
Neurological Conditions ◽  
Detailed Search

Background: Amino acids are crucially involved in a myriad of biological processes. Any aberrant changes in physiological level of amino acids often manifest in common metabolic disorders, serious neurological conditions and cardiovascular diseases. Thus, devising methods for detection of trace amounts of amino acids becomes highly elemental to their efficient clinical diagnosis. Recently, the domain of developing optical sensors for detection of amino acids has witnessed significant activity which is the focus of the current review article. Methods: We undertook a detailed search of the peer-reviewed literature that primarily deals with optical sensors for amino acids and focuses on the use of different type of materials as a sensing platform. Results: Ninety-five papers have been included in the review, majority of which deal with optical sensors. We attempt to systematically classify these contributions based on the applications of various chemical and biological scaffolds such as polymers, supramolecular assemblies, nanoparticles, DNA, heparin etc for the sensing of amino acids. This review identifies that supramolecular assemblies and nanomaterial continue to be commonly used platforms to devise sensors for amino acids followed by surfactant assemblies. Conclusion: The broad implications of amino acids in human health and diagnosis have stirred a lot of interest to develop optimized optical detection systems for amino acids in recent years, using different materials based on chemical and biological scaffolds. We have also attempted to highlight the merits and demerits of some of the noteworthy sensor systems to instigate further efforts for constructing amino acids sensor based on unconventional concepts.

Optische Schmelzbadüberwachung*/Optical monitoring of the melting bath in an Al-melting furnace

2018 ◽  
Vol 108 (11-12) ◽  
pp. 760-766
Author(s):  
S. Mohammadifard ◽  
J. Langner ◽  
M. Stonis ◽  
S. Sauke ◽  
H. Larki Harchegani ◽  
...  
Keyword(s):  
Oxide Layer ◽  
Optical Sensors ◽  
Melting Furnace ◽  
Bath Surface ◽  
Optical Monitoring ◽  
Measuring Systems ◽  
Height Change ◽  
First Time ◽  
Contact Sensors ◽  
Holding Temperature

In einem Aluminium (Al)-Schmelzofen sind der Füllstand des Schmelzbades und die Oxidschichtmenge mit Kontaktsensoren nicht überwachbar, da das Schmelzbad aufgrund der hohen Haltebereich-Temperaturen von über 600 °C nicht zugänglich ist. Deshalb wird ein Online-Überwachungssystem des Aluminium-Schmelzbades mithilfe optischer Sensoren erforscht. Dafür wird das Schmelzbad mit geeigneten optischen Messsystemen identifiziert. Schließlich werden durch Bildanalyse-Algorithmen die Höhenänderung der Schmelze herausgearbeitet. Zudem werden Oxidschichten im Ofen auf der Badoberfläche mithilfe der Algorithmen detektiert.   The melt level and oxide layer quantity in an aluminum melting furnace cannot be monitored by contact sensors, since the melting bath is not accessible due to the high holding temperature (above 600 °C). Therefore, the method of monitoring the melting bath by means of optical sensors is investigated for the first time. For this purpose, suitable optical measuring systems can be applied which will be able to record the melting bath. The height change of the melt is to be elaborated by means of image analysis and any oxide layer on the bath surface is to be detected.

scholarly journals TMCC2 Associates with Alzheimer's Disease Pathology

2021 ◽  
Author(s):  
Paul C R Hopkins ◽  
Claire Troakes ◽  
Guy Tear
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Apoe Genotype ◽  
Coiled Coil ◽  
Amyloid Protein ◽  
Neuritic Plaques ◽  
Protein Precursor ◽  
Apoe Isoforms ◽  
First Time ◽  
Differential Affinity

We previously identified Transmembrane and Coiled-Coil 2 (TMCC2) as a protein that forms complexes with both apolipoprotein E (apoE) and the amyloid protein precursor (APP) and which displayed differential affinity for apoE isoforms apoE3 and apoE4. Here we have for the first time examined TMCC2 in the human brain and found that it is affected by APOE genotype and brain region. We further observed that TMCC2 associates with the pathology of Alzheimer's disease in dense core and neuritic plaques. TMCC2 is therefore positioned to mediate impacts of apoE4 on Alzheimer's disease pathology.

scholarly journals Genome-wide interrogation of extracellular vesicle biology using barcoded miRNAs

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Albert Lu ◽  
Paulina Wawro ◽  
David W Morgens ◽  
Fernando Portela ◽  
Michael C Bassik ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Molecular Mechanisms ◽  
Membrane Vesicles ◽  
Extracellular Vesicle ◽  
Biologically Active ◽  
Vesicle Release ◽  
Guide Rnas ◽  
Disease States ◽  
Genome Wide ◽  
First Time

Extracellular vesicles mediate transfer of biologically active molecules between neighboring or distant cells, and these vesicles may play important roles in normal physiology and the pathogenesis of multiple disease states including cancer. However, the underlying molecular mechanisms of their biogenesis and release remain unknown. We designed artificially barcoded, exosomal microRNAs (bEXOmiRs) to monitor extracellular vesicle release quantitatively using deep sequencing. We then expressed distinct pairs of CRISPR guide RNAs and bEXOmiRs, enabling identification of genes influencing bEXOmiR secretion from Cas9-edited cells. This approach uncovered genes with unrecognized roles in multivesicular endosome exocytosis, including critical roles for Wnt signaling in extracellular vesicle release regulation. Coupling bEXOmiR reporter analysis with CRISPR-Cas9 screening provides a powerful and unbiased means to study extracellular vesicle biology and for the first time, to associate a nucleic acid tag with individual membrane vesicles.

Development of a dry-reagent-based nucleic acid-sensing platform by coupling thermostabilised LATE-PCR assay to an oligonucleotide-modified lateral flow biosensor

2015 ◽  
Vol 118 ◽  
pp. 99-105 ◽  
Author(s):  
Geik Yong Ang ◽  
Choo Yee Yu ◽  
Kok Gan Chan ◽  
Kirnpal Kaur Banga Singh ◽  
Yean Chan Yean
Keyword(s):  
Nucleic Acid ◽  
Lateral Flow ◽  
Pcr Assay ◽  
Sensing Platform

Black phosphorus nanosheets for rapid microRNA detection

Nanoscale ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 5060-5064 ◽  
Author(s):  
Jie Zhou ◽  
Zhongjun Li ◽  
Ming Ying ◽  
Maixian Liu ◽  
Xiaomei Wang ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Rapid Detection ◽  
Black Phosphorus ◽  
Sensing Platform ◽  
Microrna Detection ◽  
First Time

Herein, for the first time, a sensitive sensing platform for rapid detection of microRNA was developed by employing black phosphorus nanosheets as the fluorescence quenching material.

A sensitive enzyme-free lactic acid sensor based on NiO nanoparticles for practical applications

2019 ◽  
Vol 11 (28) ◽  
pp. 3578-3583 ◽  
Author(s):  
Sidra Amin ◽  
Aneela Tahira ◽  
Amber Solangi ◽  
Raffaello Mazzaro ◽  
Zafar Hussain Ibupoto ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Glassy Carbon ◽  
Electrochemical Sensing ◽  
Carbon Electrode ◽  
Nio Nanoparticles ◽  
Modified Glassy Carbon Electrode ◽  
Practical Applications ◽  
Sensing Platform ◽  
First Time

A facile and efficient electrochemical sensing platform has been successfully exploited for the first time for the determination of lactic acid using a nickel oxide (NiO) nanoparticle-modified glassy carbon electrode (GCE).

scholarly journals VLPs Derived from the CCMV Plant Virus Can Directly Transfect and Deliver Heterologous Genes for Translation into Mammalian Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
María V. Villagrana-Escareño ◽  
Elizabeth Reynaga-Hernández ◽  
Othir G. Galicia-Cruz ◽  
Ana L. Durán-Meza ◽  
Viridiana De la Cruz-González ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Mammalian Cells ◽  
Plant Virus ◽  
Fluorescent Protein ◽  
Genetic Material ◽  
Eukaryotic Cell ◽  
Plant Viruses ◽  
Virus Capsids ◽  
First Time ◽  
Drug Nanocarriers

Virus-like particles (VLPs) are being used for therapeutic developments such as vaccines and drug nanocarriers. Among these, plant virus capsids are gaining interest for the formation of VLPs because they can be safely handled and are noncytotoxic. A paradigm in virology, however, is that plant viruses cannot transfect and deliver directly their genetic material or other cargos into mammalian cells. In this work, we prepared VLPs with the CCMV capsid and the mRNA-EGFP as a cargo and reporter gene. We show, for the first time, that these plant virus-based VLPs are capable of directly transfecting different eukaryotic cell lines, without the aid of any transfecting adjuvant, and delivering their nucleic acid for translation as observed by the presence of fluorescent protein. Our results show that the CCMV capsid is a good noncytotoxic container for genome delivery into mammalian cells.

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