Breaking the reduced glutathione-activated antioxidant defence for enhanced photodynamic therapy

2017 ◽  
Vol 5 (33) ◽  
pp. 6752-6761 ◽  
Author(s):  
Qi-chen Zhan ◽  
Xian-qing Shi ◽  
Xiao-hong Yan ◽  
Qian Liu ◽  
Jia-hong Zhou ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Photodynamic Therapy ◽  
Cancer Treatment ◽  
Cancer Cells ◽  
Reduced Glutathione ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Antioxidant Defence

Photodynamic therapy (PDT) has been applied in cancer treatment by utilizing reactive oxygen species (ROSs) to kill cancer cells.

Multifunctional UCNPs@MnSiO3@g-C3N4 nanoplatform: improved ROS generation and reduced glutathione levels for highly efficient photodynamic therapy

2017 ◽  
Vol 5 (12) ◽  
pp. 2456-2467 ◽  
Author(s):  
Lili Feng ◽  
Fei He ◽  
Yunlu Dai ◽  
Shili Gai ◽  
Chongna Zhong ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Photodynamic Therapy ◽  
Cancer Treatment ◽  
Reduced Glutathione ◽  
Reactive Oxygen ◽  
Ros Generation ◽  
Oxygen Species ◽  
Malignant Cells ◽  
Novel Technique ◽  
Highly Efficient

Photodynamic therapy (PDT) is a novel technique that has been extensively employed in cancer treatment; it utilizes reactive oxygen species to kill malignant cells.

scholarly journals In vitro study of reactive oxygen species production during photodynamic therapy in ultrasound- pretreated cancer cells

2007 ◽  
pp. S27-S32
Author(s):  
H Kolářová ◽  
R Bajgár ◽  
K Tománková ◽  
E Krestyn ◽  
L Doležal ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Reactive Oxygen ◽  
In Vitro Study ◽  
Molecular Probe ◽  
Breast Adenocarcinoma ◽  
Ros Production ◽  
Oxygen Species

Several recent studies bring evidence of cell death enhancement in photodynamic compound loaded cells by ultrasonic treatment. There are a number of hypotheses suggesting the mechanism of the harmful ultrasonic effect. One of them considers a process in the activation of photosensitizers by ultrasonic energy. Because the basis of the photodynamic damaging effect on cells consists in the production of reactive oxygen species (ROS), we focused our study on whether the ultrasound can increase ROS production within cancer cells. Particularly, we studied ROS formation in ultrasound pretreated breast adenocarcinoma cells during photodynamic therapy in the presence of chloroaluminum phthalocyanine disulfonate (ClAlPcS2). Production of ROS was investigated by the molecular probe CM-H2DCFDA. Our results show that ClAlPcS2 induces higher ROS production in the ultrasound pretreated cell lines at a concentration of 100 microM and light intensity of 2 mW/cm2. We also observed a dependence of ROS production on photosensitizer concentration and light dose. These results demonstrate that the photodynamic effect on breast cancer cells can be enhanced by ultrasound pretreatment.

NIR-driven graphitic-phase carbon nitride nanosheets for efficient bioimaging and photodynamic therapy

2016 ◽  
Vol 4 (48) ◽  
pp. 8000-8008 ◽  
Author(s):  
Lili Feng ◽  
Fei He ◽  
Guixin Yang ◽  
Shili Gai ◽  
Yunlu Dai ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Carbon Nitride ◽  
Reactive Oxygen ◽  
Anticancer Therapy ◽  
Photochemical Reactions ◽  
Oxygen Species ◽  
Carbon Nitride Nanosheets

Photodynamic therapy (PDT) is a noninvasive and promising anticancer therapy modality that utilizes the photochemical reactions of photosensitizers, upon irradiation at a specific wavelength, to yield reactive oxygen species (ROS) to impair malignant cancer cells.

Mesoporous titanium dioxide nanocarrier with magnetic-targeting and high loading efficiency for dual-modal imaging and photodynamic therapy

2017 ◽  
Vol 5 (30) ◽  
pp. 6081-6096 ◽  
Author(s):  
Qianqian Yu ◽  
Jing Sun ◽  
Xufeng Zhu ◽  
Lin Qiu ◽  
Mengmeng Xu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Titanium Dioxide ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Reactive Oxygen ◽  
High Loading ◽  
Therapeutic Modality ◽  
Magnetic Targeting ◽  
Oxygen Species ◽  
Mesoporous Titanium Dioxide

Photodynamic therapy (PDT), by producing reactive oxygen species (ROS), inhibits cancer cells and is an emerging and pioneering cancer therapeutic modality which can eliminate some of the drawbacks of other traditional anticancer therapies.

Bergenin Exhibits Hepatoprotective Activity Against Ethanol-Induced Oxidative Stress in ICR Mice

2019 ◽  
Vol 18 (4) ◽  
pp. 297-302
Author(s):  
Sriset Yollada ◽  
Chatuphonprasert Waranya ◽  
Jarukamjorn Kanokwan
Keyword(s):  
Reactive Oxygen Species ◽  
Gallic Acid ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Hepatic Injury ◽  
Reduced Glutathione ◽  
Reactive Oxygen ◽  
Hepatoprotective Activity ◽  
Oxygen Species ◽  
Hepatic Glutathione

Bergenin is a C-glucoside derivative of gallic acid but its antioxidant and hepatoprotective effects have not previously been compared with gallic acid. Male ICR mice were administered bergenin (10, 50, and 250 mg/kg/day) or gallic acid (100 mg/kg/day) for 7 consecutive days before a single administration of ethanol (5 g/kg). Liver sections were histopathologically examined. Aspartate aminotransferase, alanine aminotransferase, reactive oxygen species, and malondialdehyde levels were determined in plasma. Total glutathione, reduced glutathione, and oxidized glutathione levels were determined in liver homogenates. Ethanol induced hepatic injury with prominent histopathological markers including nuclear pyknosis and necrotic areas and this accorded with increases in the plasma levels of aspartate aminotransferase, alanine aminotransferase, reactive oxygen species, and malondialdehyde. Moreover, ethanol disturbed hepatic glutathione homeostasis by reducing glutathione stores. Hepatic injury in the ethanol-induced mice was prevented with bergenin and gallic acid by significant decreases in plasma aspartate aminotransferase, alanine aminotransferase, reactive oxygen species, and malondialdehyde levels and restoration of the hepatic glutathione profile through an increase in the reduced glutathione/oxidized glutathione ratio. Bergenin at 10 mg/kg/day showed comparable hepatoprotective activity to gallic acid in an ethanol-induced mouse model of oxidative stress. Therefore, bergenin might be a promising candidate for further development as a novel hepatoprotective product.

Reserpine Induces Apoptosis and Cell Cycle Arrest in Hormone Independent Prostate Cancer Cells through Mitochondrial Membrane Potential Failure

2019 ◽  
Vol 18 (9) ◽  
pp. 1313-1322 ◽  
Author(s):  
Manjula Devi Ramamoorthy ◽  
Ashok Kumar ◽  
Mahesh Ayyavu ◽  
Kannan Narayanan Dhiraviam
Keyword(s):  
Prostate Cancer ◽  
Reactive Oxygen Species ◽  
Cell Cycle ◽  
Membrane Potential ◽  
Cancer Cells ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Prostate Cancer Cells

Background: Reserpine, an indole alkaloid commonly used for hypertension, is found in the roots of Rauwolfia serpentina. Although the root extract has been used for the treatment of cancer, the molecular mechanism of its anti-cancer activity on hormonal independent prostate cancer remains elusive. Methods: we evaluated the cytotoxicity of reserpine and other indole alkaloids, yohimbine and ajmaline on Prostate Cancer cells (PC3) using MTT assay. We investigated the mechanism of apoptosis using a combination of techniques including acridine orange/ethidium bromide staining, high content imaging of Annexin V-FITC staining, flow cytometric quantification of the mitochondrial membrane potential and Reactive Oxygen Species (ROS) and cell cycle analysis. Results: Our results indicate that reserpine inhibits DNA synthesis by arresting the cells at the G2 phase and showed all standard sequential features of apoptosis including, destabilization of mitochondrial membrane potential, reduced production of reactive oxygen species and DNA ladder formation. Our in silico analysis further confirmed that indeed reserpine docks to the catalytic cleft of anti-apoptotic proteins substantiating our results. Conclusion: Collectively, our findings suggest that reserpine can be a novel therapeutic agent for the treatment of androgen-independent prostate cancer.

Sign in / Sign up

Export Citation Format

Share Document