NIR-driven graphitic-phase carbon nitride nanosheets for efficient bioimaging and photodynamic therapy

2016 ◽  
Vol 4 (48) ◽  
pp. 8000-8008 ◽  
Author(s):  
Lili Feng ◽  
Fei He ◽  
Guixin Yang ◽  
Shili Gai ◽  
Yunlu Dai ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Carbon Nitride ◽  
Reactive Oxygen ◽  
Anticancer Therapy ◽  
Photochemical Reactions ◽  
Oxygen Species ◽  
Carbon Nitride Nanosheets

Photodynamic therapy (PDT) is a noninvasive and promising anticancer therapy modality that utilizes the photochemical reactions of photosensitizers, upon irradiation at a specific wavelength, to yield reactive oxygen species (ROS) to impair malignant cancer cells.

scholarly journals In vitro study of reactive oxygen species production during photodynamic therapy in ultrasound- pretreated cancer cells

2007 ◽  
pp. S27-S32
Author(s):  
H Kolářová ◽  
R Bajgár ◽  
K Tománková ◽  
E Krestyn ◽  
L Doležal ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Reactive Oxygen ◽  
In Vitro Study ◽  
Molecular Probe ◽  
Breast Adenocarcinoma ◽  
Ros Production ◽  
Oxygen Species

Several recent studies bring evidence of cell death enhancement in photodynamic compound loaded cells by ultrasonic treatment. There are a number of hypotheses suggesting the mechanism of the harmful ultrasonic effect. One of them considers a process in the activation of photosensitizers by ultrasonic energy. Because the basis of the photodynamic damaging effect on cells consists in the production of reactive oxygen species (ROS), we focused our study on whether the ultrasound can increase ROS production within cancer cells. Particularly, we studied ROS formation in ultrasound pretreated breast adenocarcinoma cells during photodynamic therapy in the presence of chloroaluminum phthalocyanine disulfonate (ClAlPcS2). Production of ROS was investigated by the molecular probe CM-H2DCFDA. Our results show that ClAlPcS2 induces higher ROS production in the ultrasound pretreated cell lines at a concentration of 100 microM and light intensity of 2 mW/cm2. We also observed a dependence of ROS production on photosensitizer concentration and light dose. These results demonstrate that the photodynamic effect on breast cancer cells can be enhanced by ultrasound pretreatment.

Breaking the reduced glutathione-activated antioxidant defence for enhanced photodynamic therapy

2017 ◽  
Vol 5 (33) ◽  
pp. 6752-6761 ◽  
Author(s):  
Qi-chen Zhan ◽  
Xian-qing Shi ◽  
Xiao-hong Yan ◽  
Qian Liu ◽  
Jia-hong Zhou ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Photodynamic Therapy ◽  
Cancer Treatment ◽  
Cancer Cells ◽  
Reduced Glutathione ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Antioxidant Defence

Photodynamic therapy (PDT) has been applied in cancer treatment by utilizing reactive oxygen species (ROSs) to kill cancer cells.

Mesoporous titanium dioxide nanocarrier with magnetic-targeting and high loading efficiency for dual-modal imaging and photodynamic therapy

2017 ◽  
Vol 5 (30) ◽  
pp. 6081-6096 ◽  
Author(s):  
Qianqian Yu ◽  
Jing Sun ◽  
Xufeng Zhu ◽  
Lin Qiu ◽  
Mengmeng Xu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Titanium Dioxide ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Reactive Oxygen ◽  
High Loading ◽  
Therapeutic Modality ◽  
Magnetic Targeting ◽  
Oxygen Species ◽  
Mesoporous Titanium Dioxide

Photodynamic therapy (PDT), by producing reactive oxygen species (ROS), inhibits cancer cells and is an emerging and pioneering cancer therapeutic modality which can eliminate some of the drawbacks of other traditional anticancer therapies.

Reserpine Induces Apoptosis and Cell Cycle Arrest in Hormone Independent Prostate Cancer Cells through Mitochondrial Membrane Potential Failure

2019 ◽  
Vol 18 (9) ◽  
pp. 1313-1322 ◽  
Author(s):  
Manjula Devi Ramamoorthy ◽  
Ashok Kumar ◽  
Mahesh Ayyavu ◽  
Kannan Narayanan Dhiraviam
Keyword(s):  
Prostate Cancer ◽  
Reactive Oxygen Species ◽  
Cell Cycle ◽  
Membrane Potential ◽  
Cancer Cells ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Prostate Cancer Cells

Background: Reserpine, an indole alkaloid commonly used for hypertension, is found in the roots of Rauwolfia serpentina. Although the root extract has been used for the treatment of cancer, the molecular mechanism of its anti-cancer activity on hormonal independent prostate cancer remains elusive. Methods: we evaluated the cytotoxicity of reserpine and other indole alkaloids, yohimbine and ajmaline on Prostate Cancer cells (PC3) using MTT assay. We investigated the mechanism of apoptosis using a combination of techniques including acridine orange/ethidium bromide staining, high content imaging of Annexin V-FITC staining, flow cytometric quantification of the mitochondrial membrane potential and Reactive Oxygen Species (ROS) and cell cycle analysis. Results: Our results indicate that reserpine inhibits DNA synthesis by arresting the cells at the G2 phase and showed all standard sequential features of apoptosis including, destabilization of mitochondrial membrane potential, reduced production of reactive oxygen species and DNA ladder formation. Our in silico analysis further confirmed that indeed reserpine docks to the catalytic cleft of anti-apoptotic proteins substantiating our results. Conclusion: Collectively, our findings suggest that reserpine can be a novel therapeutic agent for the treatment of androgen-independent prostate cancer.

scholarly journals Photo-Responsive Supramolecular Micelles for Controlled Drug Release and Improved Chemotherapy

2020 ◽  
Vol 22 (1) ◽  
pp. 154
Author(s):  
Fasih Bintang Ilhami ◽  
Kai-Chen Peng ◽  
Yi-Shiuan Chang ◽  
Yihalem Abebe Alemayehu ◽  
Hsieh-Chih Tsai ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Drug Release ◽  
Visible Light ◽  
Cancer Cells ◽  
Laser Irradiation ◽  
Reactive Oxygen ◽  
Controlled Drug Release ◽  
Grand Challenge ◽  
Supramolecular System ◽  
Oxygen Species

Development of stimuli-responsive supramolecular micelles that enable high levels of well-controlled drug release in cancer cells remains a grand challenge. Here, we encapsulated the antitumor drug doxorubicin (DOX) and pro-photosensitizer 5-aminolevulinic acid (5-ALA) within adenine-functionalized supramolecular micelles (A-PPG), in order to achieve effective drug delivery combined with photo-chemotherapy. The resulting DOX/5-ALA-loaded micelles exhibited excellent light and pH-responsive behavior in aqueous solution and high drug-entrapment stability in serum-rich media. A short duration (1–2 min) of laser irradiation with visible light induced the dissociation of the DOX/5-ALA complexes within the micelles, which disrupted micellular stability and resulted in rapid, immediate release of the physically entrapped drug from the micelles. In addition, in vitro assays of cellular reactive oxygen species generation and cellular internalization confirmed the drug-loaded micelles exhibited significantly enhanced cellular uptake after visible light irradiation, and that the light-triggered disassembly of micellar structures rapidly increased the production of reactive oxygen species within the cells. Importantly, flow cytometric analysis demonstrated that laser irradiation of cancer cells incubated with DOX/5-ALA-loaded A-PPG micelles effectively induced apoptotic cell death via endocytosis. Thus, this newly developed supramolecular system may offer a potential route towards improving the efficacy of synergistic chemotherapeutic approaches for cancer.

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