In situ synthesis of NIR-light emitting carbon dots derived from spinach for bio-imaging applications

2017 ◽  
Vol 5 (35) ◽  
pp. 7328-7334 ◽  
Author(s):  
Liping Li ◽  
Ruiping Zhang ◽  
Chunxiang Lu ◽  
Jinghua Sun ◽  
Lingjie Wang ◽  
...  
Keyword(s):  
Optical Properties ◽  
Carbon Dots ◽  
In Situ Synthesis ◽  
Light Emitting ◽  
Nir Light ◽  
Bio Imaging ◽  
One Step

NIR-light emitting CDs (R-CDs) were prepared using spinach as a precursor by one-step solvothermal treatment. The R-CDs exhibited great optical properties, negligible toxicity, and superior labelling capability both in vitro and in vivo.

scholarly journals In situ synthesis of stretchable and highly stable multi-color carbon-dots/polyurethane composite films for light-emitting devices

RSC Advances ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 1281-1286 ◽  
Author(s):  
Fei Lian ◽  
Chuanxi Wang ◽  
Qian Wu ◽  
Minghui Yang ◽  
Zhenyu Wang ◽  
...  
Keyword(s):  
Polymer Films ◽  
Carbon Dots ◽  
Composite Films ◽  
Optoelectronic Devices ◽  
In Situ Synthesis ◽  
Light Emitting ◽  
Light Emitting Devices ◽  
Polyurethane Composite

Stretchable, mechanically stable multi-color carbon-dots-based polymer films are in situ fabricated, and showed potential for application in optoelectronic devices.

Fluorescent carbon dots from antineoplastic drug etoposide for bioimaging in vitro and in vivo

2017 ◽  
Vol 5 (38) ◽  
pp. 7796-7800 ◽  
Author(s):  
Bin Wu ◽  
Rongrong Zhu ◽  
Mei Wang ◽  
Peng Liang ◽  
Yechang Qian ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Antineoplastic Drug ◽  
Step Method ◽  
One Step ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Carbon dots for bioimaging in vitro and in vivo were synthesized from the antineoplastic drug etoposide by a one-step method.

scholarly journals Tetracycline-regulated expression enables purification and functional analysis of recombinant connexin channels from mammalian cells

2004 ◽  
Vol 383 (1) ◽  
pp. 111-119 ◽  
Author(s):  
Irina V. KOREEN ◽  
Wafaa A. ELSAYED ◽  
Yu J. LIU ◽  
Andrew L. HARRIS
Keyword(s):  
Mammalian Cells ◽  
Cultured Cells ◽  
Expression System ◽  
Physiological Processes ◽  
Messenger Molecules ◽  
One Step ◽  
Cellular Control

Intercellular coupling mediated by gap junction channels composed of connexin protein underlies numerous physiological processes, such as cellular differentiation, tissue synchronization and metabolic homoeostasis. The distinct molecular permeability of junctional channels composed of different connexin isoforms allows cellular control of coupling via regulation of isoform expression. However, the permeability properties of most connexin isoforms have not been well characterized due to the difficulty of manipulating and measuring the diffusible concentrations of cytoplasmic messenger molecules and metabolites, and to a lack of control over channel isoform composition, in vivo. Here we present a method to express and purify active connexin hemichannels of a single isoform or a consistent ratio of two isoforms from cultured cells using the Tet-On inducible expression system and one-step anti-haemagglutinin immunoaffinity purification. The procedure yields 10–20 μg of pure connexin protein from 2.5×108 HeLa cells. The purified channels are shown to be useful for in vitro permeability analysis using well established techniques. This method has substantial advantages over existing methods for heterologous connexin expression, such as the ease of co-expression of two isoforms at a constant ratio, consistently high expression levels over many passages, and the ability to study channel properties in situ as well as in purified form. Furthermore, the generic cloning site of the new pBI-GT vector and the commercial availability of anti-haemagglutinin (clone HA-7)–agarose make this affinity tagging and purification procedure easily applicable to other proteins.

Biocompatible fluorescent carbon dots derived from roast duck for in vitro cellular and in vivo C. elegans bio-imaging

Methods ◽  
2019 ◽  
Vol 168 ◽  
pp. 76-83 ◽  
Author(s):  
Shuang Cong ◽  
Kangjing Liu ◽  
Fengzhi Qiao ◽  
Yukun Song ◽  
Mingqian Tan
Keyword(s):  
Carbon Dots ◽  
C Elegans ◽  
Bio Imaging ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

In Situ One-Step Fluorescence Labeling Strategy of Exosomes via Bioorthogonal Click Chemistry for Real-Time Exosome Tracking In Vitro and In Vivo

2020 ◽  
Vol 31 (5) ◽  
pp. 1562-1574 ◽  
Author(s):  
Sukyung Song ◽  
Man Kyu Shim ◽  
Seungho Lim ◽  
Yujeong Moon ◽  
Suah Yang ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Real Time ◽  
Fluorescence Labeling ◽  
One Step

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

Evaluation of Structure-Controlled Silk Fibroin Coatings for Orthopedic Infection and In-Situ Osteogenesis: In Vitro and In Vivo

2020 ◽  
Author(s):  
Wenhao Zhou ◽  
Teng Zhang ◽  
Jianglong Yan ◽  
QiYao Li ◽  
Panpan Xiong ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Orthopedic Infection

Carbon dots derived from Fusobacterium nucleatum for intracellular determination of Fe3+ and bioimaging both in vitro and in vivo

2021 ◽  
Author(s):  
Lijuan Liu ◽  
Shengting Zhang ◽  
Xiaodan Zheng ◽  
Hongmei Li ◽  
Qi Chen ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Fusobacterium Nucleatum ◽  
Living Cells ◽  
First Time ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.

scholarly journals Improved biorefinery pathways of marine diatoms using a water miscible ionic liquid and its colloidal solution: efficient lipid extraction and in situ synthesis of fluorescent carbon dots for bio-imaging applications

RSC Advances ◽  
2021 ◽  
Vol 11 (35) ◽  
pp. 21207-21215
Author(s):  
Paidi Murali Krishna ◽  
Veerababu Polisetti ◽  
Krishnaiah Damarla ◽  
Subir Kumar Mandal ◽  
Arvind Kumar
Keyword(s):  
Ionic Liquid ◽  
Colloidal Solution ◽  
Polar Solvent ◽  
Lipid Extraction ◽  
In Situ Synthesis ◽  
Marine Diatoms ◽  
Bio Imaging ◽  
Partition Method ◽  
Fluorescent Carbon Dots

In this study, a water-miscible ionic liquid (IL), 1-ethyl-3-methylimidazoliumacetate ([EMIM][Ac]), has been used for lipid extraction from marine diatoms Thalassiosira lundiana CSIR-CSMCRI 001 by following a non-polar solvent partition method.

Sign in / Sign up

Export Citation Format

Share Document