Robust silk fibroin/bacterial cellulose nanoribbon composite scaffolds with radial lamellae and intercalation structure for bone regeneration

2017 ◽  
Vol 5 (20) ◽  
pp. 3640-3650 ◽  
Author(s):  
Jian Chen ◽  
Ao Zhuang ◽  
Huili Shao ◽  
Xuechao Hu ◽  
Yaopeng Zhang
Keyword(s):  
Mechanical Properties ◽  
Bone Regeneration ◽  
Bacterial Cellulose ◽  
Silk Fibroin ◽  
Composite Scaffolds ◽  
Biomimetic Scaffolds

Biomimetic scaffolds with a gradient gap distance and robust mechanical properties were prepared using silk fibroin and bacterial cellulose.

scholarly journals Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds

2015 ◽  
pp. 485 ◽  
Author(s):  
Won Ho Park ◽  
Beom Su Kim ◽  
Ko Eun Park ◽  
Hyung Keun You ◽  
Jun Lee ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Silk Fibroin ◽  
Composite Scaffolds

Preparation of Silk Fibroin/Bacterial Cellulose Composite Films and their Mechanical Properties

2007 ◽  
Vol 342-343 ◽  
pp. 741-744 ◽  
Author(s):  
Rira Jung ◽  
Hyoung Joon Jin
Keyword(s):  
Mechanical Properties ◽  
Bacterial Cellulose ◽  
Silk Fibroin ◽  
Composite Films ◽  
Water Molecules ◽  
X Ray Diffraction ◽  
X Ray ◽  
The Individual ◽  
Fibroin Solution ◽  
Cellulose Composite

We prepared composite films consisting of two biocompatible materials, bacterial cellulose and silk fibroin. Aqueous silk fibroin solution and bacterial cellulose excreted by Acetobacter xylinum were used to fabricate the composite films. It was verified by field emission scanning electron microscopy and X-ray diffraction that the two components were finely blended and that the silk fibroin was crystallized during the composition of the films. The silk fibroin penetrated well between the individual fibrils of the bacterial cellulose, while the water molecules inside the pellicular bacterial cellulose were evaporating. The composite films did not dissolve in water due to the crystallization of the silk fibroin in the composite films. We also observed the change in the mechanical properties of the composite films according to the water content. The composite films became more flexible and tougher when they were dipped in water, whereas they were very brittle in the dehydrated state.

Large defect-tailored composite scaffolds for in vivo bone regeneration

2014 ◽  
Vol 29 (5) ◽  
pp. 715-727 ◽  
Author(s):  
Alfredo Ronca ◽  
Vincenzo Guarino ◽  
Maria Grazia Raucci ◽  
Francesca Salamanna ◽  
Lucia Martini ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Bone Marrow ◽  
Lactic Acid ◽  
Bone Regeneration ◽  
Platelet Rich Plasma ◽  
Bone Defects ◽  
Composite Scaffolds ◽  
Bone Morphogenetic Protein 7 ◽  
Morphogenetic Protein

The discovery of new strategies to repair large segmental bone defects is currently an open challenge for worldwide clinicians. In the treatment of critical-sized bone defects, an alternative strategy to traditional bone grafting is always more frequently the use of tailor-made scaffolds modelled on the final size and shape of the implant site. Here, poly-ε-caprolactone-based composite scaffolds including poly-l-lactic acid continuous fibres and hyaluronan derivates (i.e. HYAFF11®) have been investigated for the peculiar 3D architecture characterized by interconnected macroporous networks and tunable mechanical properties. Composite scaffolds were immersed in simulated body fluid solution in order to support in vivo tissue in-growth. Scaffolds loaded with autologous cells (bone marrow stromal cells) plus platelet-rich plasma and osteoconductive protein such bone morphogenetic protein-7 were also tested to evaluate eventual enhancement in bone regeneration. The morphological and mechanical properties of poly-l-lactic acid-reinforced composite scaffolds have been studied to identify the optimal scaffold design to match the implant-site requirements of sheep metatarsal defects. Dynamic mechanical tests allowed to underline the viscoelastic response of the scaffold – resulting in elastic moduli from 2.5 to 1.3 MPa, suitable to temporarily support the structural function of damaged bone tissue. In vivo preliminary investigations in a sheep model of metatarsus shaft defect also showed the attitude of the scaffold to promote osteogenesis, preferentially in association with bone marrow stromal cell and platelet-rich plasma, even if the highest amount of mature bone was reached in the case of scaffold loaded with human bone morphogenetic protein-7 released via hydrolytic degradation of HYAFF11® phases in the implant site.

Tough and VEGF-releasing scaffolds composed of artificial silk fibroin mats and a natural acellular matrix

RSC Advances ◽  
2015 ◽  
Vol 5 (22) ◽  
pp. 16748-16758 ◽  
Author(s):  
Zhaobo Li ◽  
Lujie Song ◽  
Xiangyu Huang ◽  
Hongsheng Wang ◽  
Huili Shao ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Silk Fibroin ◽  
Composite Scaffolds ◽  
Cell Compatibility ◽  
Acellular Matrix ◽  
Good Cell

The blend and coaxially electrospun RSF/BAMG composite scaffolds loaded VEGF exhibited good cell compatibility with improved mechanical properties.

scholarly journals Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1456
Author(s):  
Isabel Matos Oliveira ◽  
Cristiana Gonçalves ◽  
Myeong Eun Shin ◽  
Sumi Lee ◽  
Rui Luis Reis ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Mechanical Properties ◽  
Silk Fibroin ◽  
Therapeutic Efficacy ◽  
Composite Structures ◽  
Gelation Time ◽  
Polymeric Materials ◽  
Gellan Gum ◽  
Traditional Use

Rheumatoid arthritis is a rheumatic disease for which a healing treatment does not presently exist. Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine. However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation. Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties. In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty–GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA). The Ty–GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2–5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time. In vitro studies showed that Ty–GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation. Furthermore, Ty–GG/SF hydrogels with encapsulated betamethasone revealed greater therapeutic efficacy than the drug applied alone. Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis.

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