scholarly journals The enhanced angiogenesis effect of VEGF-silk fibroin nanospheres-BAMG scaffold composited with adipose derived stem cells in a rabbit model

RSC Advances ◽  
2018 ◽  
Vol 8 (27) ◽  
pp. 15158-15165
Author(s):  
Dongliang Zhang ◽  
Nailong Cao ◽  
Shukui Zhou ◽  
Zhong Chen ◽  
Xinru Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Silk Fibroin ◽  
Rabbit Model ◽  
Vascular Endothelial ◽  
Adipose Derived Stem Cells ◽  
Acellular Matrix ◽  
Endothelial Growth Factor

The adipose derived stem cells (ADSCs) was composited with vascular endothelial growth factor (VEGF)-silk fibroin (SF) nanospheres-bladder acellular matrix graft (BAMG) scaffold to repair bladder defect in rabbits.

scholarly journals Overexpressed vascular endothelial growth factor in adipose derived stem cells attenuates fibroblasts and skin injuries by ultraviolet radiation

2019 ◽  
Vol 39 (7) ◽  
Author(s):  
Xiaoyuan Xie ◽  
Yina Wang ◽  
Yue Xia ◽  
Yueping Mao
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Flow Cytometry ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Uv Radiation ◽  
Vascular Endothelial ◽  
Adipose Derived Stem Cells ◽  
Vegf Mrna ◽  
Endothelial Growth Factor

Abstract Adipose-derived stem cells (ADSCs) and vascular endothelial growth factor (VEGF) contribute to the healing of wound. The purpose of the present study was to investigate the role of VEGF produced by ADSCs in the protection of fibroblasts and skin of mice from ultraviolet (UV) radiation. ADSCs and fibroblasts were extracted from adipose and skin on the abdomen of mice by enzyme digestion methods. ADSCs surface markers were detected using flow cytometry, and immunofluorescence was used to identify fibroblasts. The expression of VEGF in modified ADSCs with lentivirus was determined. Fibroblasts were injured by UV radiation and co-cultured with ADSCs carrying overexpressed VEGF or normal VEGF. Cell cycle was assessed by flow cytometry. Mice were treated with UV radiation dorsally and injected with ADSCs containing overexpressed VEGF or normal VEGF. mRNA and protein levels of cell senescence-related genes were measured by qPCR and western blot. It was found that ADSCs with overexpressed VEGF not only promoted the effect of ADSCs on down-regulating senescence-associated (SA)-β-Gal, p21 and matrix metalloproteinase (MMP)-1, the healing of wound injured by UV radiation and up-regulating collagen I expression in fibroblasts and wound, but also on inhibiting cell cycle arrest in fibroblasts injured by UV radiation and preventing the skin from photoaging caused by UV radiation. VEGF expression in ADSCs played a key role in protecting skin fibroblasts from ageing, which further allowed the skin to resist photoaging, thereby promoting the recovery of wound injured by UV radiation.

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