scholarly journals Fibroblast growth factor and vascular endothelial growth factor play a critical role in endotheliogenesis from human adipose-derived stem cells

2017 ◽  
Vol 65 (5) ◽  
pp. 1483-1492 ◽  
Author(s):  
Sophia Khan ◽  
Miguel A. Villalobos ◽  
Rachel L. Choron ◽  
Shaohua Chang ◽  
Spencer A. Brown ◽  
...  
Keyword(s):  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Critical Role ◽  
Vascular Endothelial ◽  
Adipose Derived Stem Cells ◽  
Fibroblast Growth ◽  
Endothelial Growth Factor

scholarly journals Microvascularization and Expression of Fibroblast Growth Factor and Vascular Endothelial Growth Factor and Their Receptors in the Mare Oviduct

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1099
Author(s):  
Pedro Pinto-Bravo ◽  
Maria Rosa Rebordão ◽  
Ana Amaral ◽  
Carina Fernandes ◽  
António Galvão ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Microvascular Density ◽  
Angiogenic Factors ◽  
Protein Abundance ◽  
Vascular Endothelial ◽  
Fibroblast Growth ◽  
Vascular Area ◽  
Endothelial Growth Factor

The oviduct presents the ideal conditions for fertilization and early embryonic development. In this study, (i) vascularization pattern; (ii) microvascular density; (iii) transcripts of angiogenic factors (FGF1, FGF2, VEGF) and their receptors—FGFR1, FGFR2, KDR, respectively, and (iv) the relative protein abundance of those receptors were assessed in cyclic mares’ oviducts. The oviductal artery, arterioles and their ramifications, viewed by means of vascular injection-corrosion, differed in the infundibulum, ampulla and isthmus. The isthmus, immunostained with CD31, presented the largest vascular area and the highest number of vascular structures in the follicular phase. Transcripts (qPCR) and relative protein abundance (Western blot) of angiogenic factors fibroblast growth factor 1 (FGF1) and 2 (FGF2) and vascular endothelial growth factor (VEGF), and their respective receptors (FGFR1, FGFR2, VEGFR2 = KDR), were present in all oviduct portions throughout the estrous cycle. Upregulation of the transcripts of angiogenic receptors FGF1 and FGFR1 in the ampulla and isthmus and of FGF2 and KDR in the isthmus were noted. Furthermore, in the isthmus, the relative protein abundance of FGFR1 and KDR was the highest. This study shows that the equine oviduct presents differences in microvascular density in its three portions. The angiogenic factors VEGF, FGF1, FGF2 and their respective receptors are expressed in all studied regions of the mare oviduct, in agreement with microvascular patterns.

Sign in / Sign up

Export Citation Format

Share Document