scholarly journals Detecting KRAS mutations in peripheral blood of colorectal cancer patients by peptide nucleic acid clamp PCR

2012 ◽  
Vol 4 (5) ◽  
pp. 790-794 ◽  
Author(s):  
SHOZO MIYANO ◽  
KISABURO HANAZAWA ◽  
TOSHIAKI KITABATAKE ◽  
MINORU FUJISAWA ◽  
KUNIAKI KOJIMA
Keyword(s):  
Colorectal Cancer ◽  
Nucleic Acid ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Peptide Nucleic Acid ◽  
Kras Mutations ◽  
Colorectal Cancer Patients

scholarly journals Serum sE-selectin levels and carcinoembryonic antigen mRNA-expressing cells in peripheral blood as prognostic factors in colorectal cancer patients

Cancer ◽  
2010 ◽  
Vol 116 (12) ◽  
pp. 2913-2921 ◽  
Author(s):  
Patrizia Ferroni ◽  
Mario Roselli ◽  
Antonella Spila ◽  
Roberta D'Alessandro ◽  
Ilaria Portarena ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Prognostic Factors ◽  
Carcinoembryonic Antigen ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Colorectal Cancer Patients

scholarly journals KRAS and NRAS mutational gene profile of metastatic colorectal cancer patients in Jordan: Molecular spectrum, frequency and distribution pattern

2019 ◽  
Author(s):  
Muhammad Awidi ◽  
Nidaa Ababneh ◽  
Maha Shomaf ◽  
Feras Al Fararjeh ◽  
Laila Owaidi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Metastatic Colorectal Cancer ◽  
Distribution Pattern ◽  
Cancer Patients ◽  
Molecular Spectrum ◽  
Kras Mutations ◽  
Exon 2 ◽  
Ras Mutations ◽  
Colorectal Cancer Patients ◽  
Kras Exon

Abstract Background A constitutively active RAS protein in the absence of stimulation of the epidermal growth factor receptor (EGFR) is the result of mutations in KRAS and NRAS genes. Mutations in the KRAS exon 2 and outside exon 2 have been found to predict the resistance to anti-EGFR monoclonal therapy. A substantial proportion of metastatic colorectal cancer cases (mCRC) exhibit RAS mutations outside KRAS exon 2, particularly in KRAS exon 3 and 4 and NRAS exons 2, 3. No data about RAS mutations outside KRAS exon 2 are available for Jordanian patients with mCRC. We aim to study the molecular spectrum, frequency, and distribution pattern of KRAS and NRAS mutations in Jordanian patients with mCRC. Methods A cohort of 190 Jordanian metastatic colorectal cancer patients were enrolled in the trial. We detected mutations in exon 2 of the KRAS and NRAS gene as well as mutations outside of exon 2 using the StripAssay technique. The KRAS StripAssay covered 29 mutations and 22 NRAS mutations. Results Mutations were observed in 92 (48.42%) cases, and KRAS exon 2 accounted for 76 cases (83.69%). KRAS G12D was the most common mutation, occurring in 18 cases, followed by KRAS G12A in 16 cases, and G12T in 13 cases. Mutations outside of KRAS exon 2 represented 16.3% of the mutated cases. Among those, 6 cases (6.48%) carried mutations in NRAS exon 2, 3 and 10 cases (10.87%) in KRAS exon 3 and 4. Conclusion The frequency of NRAS and KRAS mutations outside of exon 2 appears to be higher in Jordanian patients in comparison with patients from western countries. KRAS mutations outside of exon 2 should be tested routinely to identify patients who should not be treated with anti-EGFR antibodies.

Quantitative analysis of TEM-8 and CEA tumor markers indicating free tumor cells in the peripheral blood of colorectal cancer patients

2011 ◽  
Vol 26 (10) ◽  
pp. 1265-1270 ◽  
Author(s):  
Reza Raeisossadati ◽  
Moein Farshchian ◽  
Azita Ganji ◽  
Alieza Tavassoli ◽  
Arash Velayati ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Quantitative Analysis ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Tumor Markers ◽  
Peripheral Blood ◽  
Colorectal Cancer Patients

scholarly journals KRAS Mutations in Colorectal Cancer Patients in Serbia: 5 Years Experience of Central Testing

2014 ◽  
Vol 25 ◽  
pp. ii74
Author(s):  
Jankovic Radmila ◽  
Brotto Ksenija ◽  
Malisic Emina ◽  
Krivokuca Ana ◽  
Cavic Milena ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Patients ◽  
Kras Mutations ◽  
Colorectal Cancer Patients

Early detection of cancer/testis mRNAs in tumor cells circulating in the peripheral blood of colorectal cancer patients

2012 ◽  
Vol 46 (5) ◽  
pp. 687-692 ◽  
Author(s):  
D. V. Novikov ◽  
T. V. Belova ◽  
E. S. Plekhanova ◽  
O. S. Yanchenko ◽  
V. V. Novikov
Keyword(s):  
Colorectal Cancer ◽  
Early Detection ◽  
Cancer Patients ◽  
Tumor Cells ◽  
Peripheral Blood ◽  
Early Detection Of Cancer ◽  
Colorectal Cancer Patients ◽  
Cancer Testis
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