scholarly journals Wash-free colorimetric homogeneous immunoassay for Zygosaccharomyces rouxii

RSC Advances ◽  
2017 ◽  
Vol 7 (55) ◽  
pp. 34307-34314 ◽  
Author(s):  
Hong Guo ◽  
Ya Hong Yuan ◽  
Chen Niu ◽  
Zhouli Wang ◽  
Yue Qiu ◽  
...  
Keyword(s):  
Zygosaccharomyces Rouxii ◽  
Homogeneous Immunoassay

A rapid and convenient AuNP-based method for detecting large microbes was developed.

scholarly journals ZrFsy1, a High-Affinity Fructose/H+ Symporter from Fructophilic Yeast Zygosaccharomyces rouxii

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e68165 ◽  
Author(s):  
Maria José Leandro ◽  
Hana Sychrová ◽  
Catarina Prista ◽  
Maria C. Loureiro-Dias
Keyword(s):  
Zygosaccharomyces Rouxii ◽  
High Affinity

A colorimetric homogeneous immunoassay system for the C-reactive protein

The Analyst ◽  
2013 ◽  
Vol 138 (5) ◽  
pp. 1538 ◽  
Author(s):  
Ju-Young Byun ◽  
Yong-Beom Shin ◽  
Dong-Myung Kim ◽  
Min-Gon Kim
Keyword(s):  
C Reactive Protein ◽  
Reactive Protein ◽  
Homogeneous Immunoassay

Regulation of aspartate-derived amino-acid metabolism in Zygosaccharomyces rouxii compared to Saccharomyces cerevisiae

2000 ◽  
Vol 27 (1-2) ◽  
pp. 151-156 ◽  
Author(s):  
Catrinus van der Sluis ◽  
Bart A Smit ◽  
Sybe Hartmans ◽  
Eelko G ter Schure ◽  
Johannes Tramper ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Zygosaccharomyces Rouxii

A Single-Molecule Homogeneous Immunoassay by Counting Spatially “Overlapping” Two-Color Quantum Dots with Wide-Field Fluorescence Microscopy

ACS Sensors ◽  
2018 ◽  
Vol 3 (12) ◽  
pp. 2644-2650 ◽  
Author(s):  
Xiaojun Liu ◽  
Conghui Huang ◽  
Chenghua Zong ◽  
Aiye Liang ◽  
Zhangjian Wu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Wide Field ◽  
Homogeneous Immunoassay

Heat preadaptation improved the ability of Zygosaccharomyces rouxii to salt stress: a combined physiological and transcriptomic analysis

2020 ◽  
Vol 105 (1) ◽  
pp. 259-270
Author(s):  
Dingkang Wang ◽  
Min Zhang ◽  
Jun Huang ◽  
Rongqing Zhou ◽  
Yao Jin ◽  
...  
Keyword(s):  
Salt Stress ◽  
Transcriptomic Analysis ◽  
Zygosaccharomyces Rouxii

scholarly journals Mechanism for Restoration of Fertility in Hybrid Zygosaccharomyces rouxii Generated by Interspecies Hybridization

2017 ◽  
Vol 83 (21) ◽  
Author(s):  
Jun Watanabe ◽  
Kenji Uehara ◽  
Yoshinobu Mogi ◽  
Yuichiro Tsukioka
Keyword(s):  
Mating Type ◽  
Whole Genome Duplication ◽  
Gene Loss ◽  
Genome Duplication ◽  
Draft Genome ◽  
Content Type ◽  
Zygosaccharomyces Rouxii ◽  
Interspecies Hybridization ◽  
Random Gene Loss ◽  
Interspecies Hybrid

ABSTRACT The mechanism of whole-genome duplication (WGD) in yeast has been intensively studied because it has a large impact on yeast evolution. WGD has shaped the genomic architecture of modern Saccharomyces cerevisiae; however, the mechanism for restoring fertility after interspecies hybridization, which would be involved in the process of WGD, has not been thoroughly elucidated. In this study, we obtained a draft genome sequence of the salt-tolerant yeast Zygosaccharomyces rouxii NBRC110957 and revealed that it is a hybrid lineage of Z. rouxii (allodiploid) with two subgenomes equivalent to NBRC1876. Because this allodiploid yeast can mate with other allodiploid strains and form spores, it can be a good model of restoring fertility after interspecies hybridization. We observed that NBRC110957 and NBRC1876 contain six mating-type-like (MTL) loci. There are no large deletions or deleterious mutations in MTL loci, except for several-base-pair deletions in the X region in certain MTL loci. We also assigned only one mating-type (MAT) locus that exclusively determines mating types from six MTL loci. These results suggest that it is possible to recover mating competence regardless of whether cells lose one MAT locus through random gene loss by mitotically dividing after interspecies hybridization. Moreover, we propose that perturbation of gene expression and substantial breakdown of MAT heterozygosity caused by chromosomal rearrangement at MTL loci play roles in restoring the mating competence of allodiploids. This scenario can provide a mechanism for restoring fertility after interspecies hybridization that is compatible with random gene loss models and suggests genomic plasticity during WGD in yeast. IMPORTANCE A whole-genome duplication occurred in an ancestor of the baker's yeast Saccharomyces cerevisiae. The origins of this complex and multifaceted process, which requires intra- or interspecies hybridization followed by dysfunction of one mating-type (MAT) locus to regain mating competence, has not been thoroughly elucidated. In this study, we provide a mechanism for regaining fertility in an interspecies hybrid, Zygosaccharomyces rouxii. The draft genome sequence analysis and mating test showed that the Z. rouxii strain used in this study is an intact interspecies hybrid, suggesting that it is possible to recover fertility regardless of whether cells lose one MAT locus.

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