Development of a homogeneous immunoassay based on the AlphaLISA method for the detection of chloramphenicol in milk, honey and eggs

2012 ◽  
Vol 92 (9) ◽  
pp. 1944-1947 ◽  
Author(s):  
Yi Zhang ◽  
Biao Huang ◽  
Jue Zhang ◽  
Ke Wang ◽  
Jian Jin
Keyword(s):  
Homogeneous Immunoassay

A colorimetric homogeneous immunoassay system for the C-reactive protein

The Analyst ◽  
2013 ◽  
Vol 138 (5) ◽  
pp. 1538 ◽  
Author(s):  
Ju-Young Byun ◽  
Yong-Beom Shin ◽  
Dong-Myung Kim ◽  
Min-Gon Kim
Keyword(s):  
C Reactive Protein ◽  
Reactive Protein ◽  
Homogeneous Immunoassay

scholarly journals Wash-free colorimetric homogeneous immunoassay for Zygosaccharomyces rouxii

RSC Advances ◽  
2017 ◽  
Vol 7 (55) ◽  
pp. 34307-34314 ◽  
Author(s):  
Hong Guo ◽  
Ya Hong Yuan ◽  
Chen Niu ◽  
Zhouli Wang ◽  
Yue Qiu ◽  
...  
Keyword(s):  
Zygosaccharomyces Rouxii ◽  
Homogeneous Immunoassay

A rapid and convenient AuNP-based method for detecting large microbes was developed.

A Single-Molecule Homogeneous Immunoassay by Counting Spatially “Overlapping” Two-Color Quantum Dots with Wide-Field Fluorescence Microscopy

ACS Sensors ◽  
2018 ◽  
Vol 3 (12) ◽  
pp. 2644-2650 ◽  
Author(s):  
Xiaojun Liu ◽  
Conghui Huang ◽  
Chenghua Zong ◽  
Aiye Liang ◽  
Zhangjian Wu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Wide Field ◽  
Homogeneous Immunoassay

Use of a reversed-phase evaporation vesicle formulation for a homogeneous liposome immunoassay.

1986 ◽  
Vol 32 (9) ◽  
pp. 1687-1691 ◽  
Author(s):  
E Canova-Davis ◽  
C T Redemann ◽  
Y P Vollmer ◽  
V T Kung
Keyword(s):  
Enzyme Activity ◽  
Colorimetric Detection ◽  
Reversed Phase ◽  
Homogeneous Immunoassay ◽  
Bacterial Enzyme ◽  
Liposome Preparation ◽  
Enzyme Molecules ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Relevant Range ◽  
Serum Components

Abstract Complement-mediated release of enzyme molecules from reversed-phase evaporation vesicles serves as the basis of the sensitive homogeneous immunoassay reported here. We found it necessary to co-entrap the substrate glucose 6-phosphate with the bacterial enzyme glucose-6-phosphate dehydrogenase (EC 1.1.1.49) to protect enzyme activity during liposome preparation. Enzyme can be released specifically from these liposomes by incubation with antibody and complement. the enzyme is not merely available to substrate but is actually physically free of the liposomes. Inhibition of this complement-mediated lysis by theophylline is the basis for the homogeneous liposome immunoassay described. The assay results vary linearly with theophylline concentrations in plasma in the clinically relevant range, and serum components do not interfere. The reagents in the assay kit are stable for at least seven months when stored at 5 degrees C. No nontheophylline compounds reacted significantly with the antiserum used. The assay can be run in a kinetic format, with either ultraviolet or colorimetric detection.

Dual-Color Fluorescence and Homogeneous Immunoassay for the Determination of Human Enterovirus 71

2011 ◽  
Vol 83 (19) ◽  
pp. 7316-7322 ◽  
Author(s):  
Lu Chen ◽  
Xiaowei Zhang ◽  
Cuiling Zhang ◽  
Guohua Zhou ◽  
Wanpo Zhang ◽  
...  
Keyword(s):  
Enterovirus 71 ◽  
Human Enterovirus ◽  
Human Enterovirus 71 ◽  
Homogeneous Immunoassay ◽  
Dual Color
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