scholarly journals Aptamer-based fluorescent detection of ochratoxin A by quenching of gold nanoparticles

RSC Advances ◽  
2017 ◽  
Vol 7 (27) ◽  
pp. 16290-16294 ◽  
Author(s):  
Xin Lv ◽  
Yuanfu Zhang ◽  
Guofu Liu ◽  
Lingyun Du ◽  
Shuhao Wang
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Quenching ◽  
Ochratoxin A ◽  
Low Cost ◽  
Fluorescent Detection ◽  
Quenching Effect ◽  
Highly Sensitive ◽  
Sensitive Method

A simple, rapid, low cost and highly sensitive method for the detection of ochratoxin A (OTA) was developed based on the principle that dispersed AuNPs show a better fluorescence quenching effect than aggregated AuNPs.

scholarly journals Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Chi-Fang Peng ◽  
Xiao-Hui Duan ◽  
Qiu-Li Pan ◽  
Li-Qiang Liu ◽  
Feng Xue
Keyword(s):  
Gold Nanoparticles ◽  
Rapid Screening ◽  
Veterinary Drug ◽  
Animal Tissues ◽  
Elisa Method ◽  
Veterinary Drug Residue ◽  
Highly Sensitive ◽  
Chicken Tissue ◽  
Signal Probe ◽  
Sensitive Method

It is challenging to obtain a highly sensitive enzyme-linked sorbent immunoassay (ELISA) method for the rapid screening of veterinary drug residue in animal tissues. Here we present that a simple and sensitive detection for sulfadimethoxine (SDM) residue in animal tissues was achieved by utilizing a bioconjugate of gold nanoparticles and enzyme-labeled antibody as signal probe in enzyme-linked sorbent immunoassay (ELISA). The developed nano-ELISA has increased the sensitivity of a traditional ELISA for SDM by 20-fold. The sensitivity of this ELISA was 5 pg/mL in buffer, and the detection limit (LOD) of 0.2 µg/kg can be obtained after chicken liver was simply extracted by buffer. This simple and sensitive method can be used to improve the sensitivity of ELISA methods for various small molecule contaminants.

scholarly journals Highly Sensitive and Selective Colorimetric Detection of Methylmercury Based on DNA Functionalized Gold Nanoparticles

Sensors ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 2679 ◽  
Author(s):  
Zheng-Jun Xie ◽  
Xian-Yu Bao ◽  
Chi-Fang Peng
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Limit Of Detection ◽  
Low Cost ◽  
Colorimetric Detection ◽  
Colorimetric Method ◽  
Optimal Conditions ◽  
Surface Deposition ◽  
Highly Sensitive ◽  
Dna Strand

A new colorimetric detection of methylmercury (CH3Hg+) was developed, which was based on the surface deposition of Hg enhancing the catalytic activity of gold nanoparticles (AuNPs). The AuNPs were functionalized with a specific DNA strand (HT7) recognizing CH3Hg+, which was used to capture and separate CH3Hg+ by centrifugation. It was found that the CH3Hg+ reduction resulted in the deposition of Hg onto the surface of AuNPs. As a result, the catalytic activity of the AuNPs toward the chromogenic reaction of 3,3,5,5-tetramethylbenzidine (TMB)-H2O2 was remarkably enhanced. Under optimal conditions, a limit of detection of 5.0 nM was obtained for CH3Hg+ with a linear range of 10–200 nM. We demonstrated that the colorimetric method was fairly simple with a low cost and can be conveniently applied to CH3Hg+ detection in environmental samples.

scholarly journals A Nanocomposite Based on Reduced Graphene and Gold Nanoparticles for Highly Sensitive Electrochemical Detection of Pseudomonas aeruginosa through Its Virulence Factors

Materials ◽  
2019 ◽  
Vol 12 (7) ◽  
pp. 1180 ◽  
Author(s):  
Islem Gandouzi ◽  
Mihaela Tertis ◽  
Andreea Cernat ◽  
Dalila Saidane-Mosbahi ◽  
Aranka Ilea ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Gold Nanoparticles ◽  
Bacterial Infections ◽  
Electrochemical Sensors ◽  
Tap Water ◽  
Low Cost ◽  
Biological Contamination ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
Higher Sensitivity

Pyoverdine is a fluorescent siderophore produced by Pseudomonas aeruginosa that can be considered as a detectable marker in nosocomial infections. The presence of pyoverdine in water can be directly linked to the presence of the P. aeruginosa, thus being a nontoxic and low-cost marker for the detection of biological contamination. A novel platform was developed and applied for the electrochemical selective and sensitive detection of pyoverdine, based on a graphene/graphite-modified screen-printed electrode (SPE) that was electrochemically reduced and decorated with gold nanoparticles (NPs). The optimized sensor presenting higher sensitivity towards pyoverdine was successfully applied for its detection in real samples (serum, saliva, and tap water), in the presence of various interfering species. The excellent analytical performances underline the premises for an early diagnosis kit of bacterial infections based on electrochemical sensors.

scholarly journals Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles

Toxins ◽  
2015 ◽  
Vol 7 (12) ◽  
pp. 5377-5385 ◽  
Author(s):  
Yunxia Luan ◽  
Jiayi Chen ◽  
Cheng Li ◽  
Gang Xie ◽  
Hailong Fu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Ochratoxin A ◽  
Colorimetric Detection ◽  
Label Free ◽  
Highly Sensitive

Sensitive fluorescence detection of mercury(ii) in aqueous solution by the fluorescence quenching effect of MoS2 with DNA functionalized carbon dots

The Analyst ◽  
2016 ◽  
Vol 141 (22) ◽  
pp. 6344-6352 ◽  
Author(s):  
K. Srinivasan ◽  
K. Subramanian ◽  
K. Murugan ◽  
K. Dinakaran
Keyword(s):  
Aqueous Solution ◽  
Fluorescence Quenching ◽  
Fluorescence Detection ◽  
Carbon Dots ◽  
Sensitive Detection ◽  
Quenching Effect ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Fluorescence Spectrometer

Highly sensitive detection of mercury(ii) ions by using a fluorescence spectrometer using MoS2 nanosheets and DNA labeled carbon dots is presented.

A fast and highly sensitive method for the detection of canine distemper virus by the naked eye

2015 ◽  
Vol 7 (6) ◽  
pp. 2264-2267 ◽  
Author(s):  
Caroline R. Basso ◽  
Claudia C. Tozato ◽  
João Pessoa A. Junior ◽  
Valber A. Pedrosa
Keyword(s):  
Gold Nanoparticle ◽  
Canine Distemper Virus ◽  
Low Cost ◽  
Canine Distemper ◽  
Naked Eye ◽  
Highly Sensitive ◽  
Sensitive Method

An easy, low cost method for the detection of canine distemper virus (CDV) that requires no instrumentation and uses gold nanoparticle-labeled antibodies is presented.

scholarly journals A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Guanhua Xun ◽  
Stephan Thomas Lane ◽  
Vassily Andrew Petrov ◽  
Brandon Elliott Pepa ◽  
Huimin Zhao
Keyword(s):  
Limit Of Detection ◽  
Low Cost ◽  
High Volume ◽  
N Gene ◽  
Testing System ◽  
Target Sequence ◽  
One Pot ◽  
Sequence Detection ◽  
Highly Sensitive ◽  
Speed Accuracy

AbstractThe need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/μL and 1.09 copies/μL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities.

Synthesis and application of polycation-stabilized gold nanoparticles as a highly sensitive sensor for molecular cysteine determination

2021 ◽  
pp. 106481
Author(s):  
Quang Khanh Nguyen ◽  
Thi Hieu Hoang ◽  
Xuan Thanh Bui ◽  
Thi Anh Huong Nguyen ◽  
Tien Duc Pham ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Highly Sensitive ◽  
Sensitive Sensor
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