A fast and highly sensitive method for the detection of canine distemper virus by the naked eye

2015 ◽  
Vol 7 (6) ◽  
pp. 2264-2267 ◽  
Author(s):  
Caroline R. Basso ◽  
Claudia C. Tozato ◽  
João Pessoa A. Junior ◽  
Valber A. Pedrosa
Keyword(s):  
Gold Nanoparticle ◽  
Canine Distemper Virus ◽  
Low Cost ◽  
Canine Distemper ◽  
Naked Eye ◽  
Highly Sensitive ◽  
Sensitive Method

An easy, low cost method for the detection of canine distemper virus (CDV) that requires no instrumentation and uses gold nanoparticle-labeled antibodies is presented.

scholarly journals Aptamer-based fluorescent detection of ochratoxin A by quenching of gold nanoparticles

RSC Advances ◽  
2017 ◽  
Vol 7 (27) ◽  
pp. 16290-16294 ◽  
Author(s):  
Xin Lv ◽  
Yuanfu Zhang ◽  
Guofu Liu ◽  
Lingyun Du ◽  
Shuhao Wang
Keyword(s):  
Gold Nanoparticles ◽  
Fluorescence Quenching ◽  
Ochratoxin A ◽  
Low Cost ◽  
Fluorescent Detection ◽  
Quenching Effect ◽  
Highly Sensitive ◽  
Sensitive Method

A simple, rapid, low cost and highly sensitive method for the detection of ochratoxin A (OTA) was developed based on the principle that dispersed AuNPs show a better fluorescence quenching effect than aggregated AuNPs.

scholarly journals Fluorescence and Naked-Eye Detection of Pb2+ in Drinking Water Using a Low-Cost Ionophore Based Sensing Scheme

2018 ◽  
Author(s):  
Aron Hakonen ◽  
Niklas Strömberg
Keyword(s):  
Drinking Water ◽  
Tap Water ◽  
Low Cost ◽  
Consumer Products ◽  
Lead Ion ◽  
Eye Detection ◽  
Naked Eye ◽  
Highly Sensitive ◽  
Naked Eye Detection

Drinking water contamination of lead from various environmental sources, leaching consumer products and intrinsic water-pipe infrastructure is still today a matter of great concern. Therefore, new highly sensitive and convenient Pb2+ measurement schemes are necessary, especially for in-situ measurements at a low-cost. Within this work dye/ionophore/Pb2+ co-extraction and effective water phase de-colorization was utilized for highly sensitive lead measurements and sub-ppb naked-eye detection. A low-cost ionophore Benzo-18-Crown-6-ether was used, and a simple test-tube mix and separate procedure was developed. Instrumental detection limits were in the low ppt region (LOD=3, LOQ=10), and naked-eye detection was 500 ppt. Note, however, that this sensing scheme still has improvement potential as concentrations of fluorophore and ionophore were not optimized. Artificial tap-water samples, leached by a standardized method, demonstrated drinking water application. Implications for this method are convenient in-situ lead ion measurements.

scholarly journals Protective levels of canine distemper virus antibody in an urban dog population using plaque reduction neutralization test

2004 ◽  
Vol 71 (3) ◽  
Author(s):  
O.I. Oyedele ◽  
D.O. Oluwayelu ◽  
S.I.B. Cadmus ◽  
F.D. Adu
Keyword(s):  
Neutralizing Antibodies ◽  
Canine Distemper Virus ◽  
Neutralization Test ◽  
Neutralization Assay ◽  
Canine Distemper ◽  
Virus Antibody ◽  
Post Vaccination ◽  
Blood Samples ◽  
Highly Sensitive ◽  
Positive Serum

Blood samples from 50 dogs were collected at three veterinary clinics in Ibadan and Abuja, Nigeria and the serum from each sample was evaluated serologically for neutralizing antibodies against canine distemper virus (CDV) by the highly sensitive plaque reduction (PRN) neutralization assay. Thirteen dogs had plaque reduction neutralization titres of 0-100, seven had titres of 100-1 000 while 30 had titres ranging from 1 000-6 000. The PRN titres of vaccinated dogs were found to be significantly higher than unvaccinated dogs. The widespread use of the highly reproducible PRN test for the evaluation of antibody response to CDV may be very important in the generation of international CDV positive serum standards that should help to improve pre-and post-vaccination testing of dogs worldwide.

scholarly journals Fluorescence and Naked-Eye Detection of Pb2+ in Drinking Water Using a Low-Cost Ionophore Based Sensing Scheme

2018 ◽  
Author(s):  
Aron Hakonen ◽  
Niklas Strömberg
Keyword(s):  
Drinking Water ◽  
Tap Water ◽  
Low Cost ◽  
Consumer Products ◽  
Lead Ion ◽  
Eye Detection ◽  
Naked Eye ◽  
Highly Sensitive ◽  
Naked Eye Detection

Drinking water contamination of lead from various environmental sources, leaching consumer products and intrinsic water-pipe infrastructure is still today a matter of great concern. Therefore, new highly sensitive and convenient Pb2+ measurement schemes are necessary, especially for in-situ measurements at a low-cost. Within this work dye/ionophore/Pb2+ co-extraction and effective water phase de-colorization was utilized for highly sensitive lead measurements and sub-ppb naked-eye detection. Low-cost ionophore Benzo-18-Crown-6-ether was used, and a simple test-tube mix, shake and separate procedure was developed. Instrumental detection limits were in the low ppt region (LOD=3, LOQ=10), and naked-eye detection was 500 ppt. Note, however, that this sensing scheme still has improvement potential as concentrations of fluorophore and ionophore were not optimized. Artificial tap-water samples, leached by a standardized method, demonstrated drinking water application. Implications for this method are convenient in-situ lead ion measurements.

scholarly journals Fluorescence and Naked-Eye Detection of Pb2+ in Drinking Water Using a Low-Cost Ionophore Based Sensing Scheme

Chemosensors ◽  
2018 ◽  
Vol 6 (4) ◽  
pp. 51 ◽  
Author(s):  
Aron Hakonen ◽  
Niklas Strömberg
Keyword(s):  
Drinking Water ◽  
Tap Water ◽  
Low Cost ◽  
Consumer Products ◽  
Lead Ion ◽  
Eye Detection ◽  
Naked Eye ◽  
Highly Sensitive ◽  
Naked Eye Detection

Drinking water contamination of lead from various environmental sources, leaching consumer products, and intrinsic water-pipe infrastructure is still today a matter of great concern. Therefore, new highly sensitive and convenient Pb2+ measurement schemes are necessary, especially for in-situ measurements at a low cost. Within this work dye/ionophore/Pb2+ co-extraction and effective water phase de-colorization was utilized for highly sensitive lead measurements and sub-ppb naked-eye detection. A low-cost ionophore Benzo-18-Crown-6-ether was used, and a simple test-tube mix and separate procedure was developed. Instrumental detection limits were in the low ppt region (LOD = 3, LOQ = 10), and naked-eye detection was 500 ppt. Note, however, that this sensing scheme still has improvement potential as concentrations of fluorophore and ionophore were not optimized. Artificial tap-water samples, leached by a standardized method, demonstrated drinking water application. Implications for this method are convenient in-situ lead ion measurements.

Highly sensitive ‘naked-eye’ colorimetric detection of thiourea using gold nanoparticles

2015 ◽  
Vol 7 (12) ◽  
pp. 4927-4933 ◽  
Author(s):  
Yi-Liang Cao ◽  
Yan Li ◽  
Fei Zhang ◽  
Jian-Zhong Huo ◽  
Xiao-Jun Zhao
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Colorimetric Detection ◽  
Sensitive Detection ◽  
Naked Eye ◽  
Colorimetric System ◽  
Highly Sensitive ◽  
Aqueous Environments

A non aggregation colorimetric system was developed for the fast and sensitive detection of thiourea in aqueous environments using an unlabeled gold nanoparticle probe.

scholarly journals A new, highly sensitive method for the detection and quantification of penicillin-binding proteins

1993 ◽  
Vol 291 (1) ◽  
pp. 19-21 ◽  
Author(s):  
M Galleni ◽  
B Lakaye ◽  
S Lepage ◽  
M Jamin ◽  
I Thamm ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Binding Proteins ◽  
New Method ◽  
Bacterial Cells ◽  
Penicillin Binding Proteins ◽  
Naked Eye ◽  
Highly Sensitive ◽  
Direct Labelling ◽  
Sensitive Method ◽  
Detection And Quantification

A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.

scholarly journals A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Guanhua Xun ◽  
Stephan Thomas Lane ◽  
Vassily Andrew Petrov ◽  
Brandon Elliott Pepa ◽  
Huimin Zhao
Keyword(s):  
Limit Of Detection ◽  
Low Cost ◽  
High Volume ◽  
N Gene ◽  
Testing System ◽  
Target Sequence ◽  
One Pot ◽  
Sequence Detection ◽  
Highly Sensitive ◽  
Speed Accuracy

AbstractThe need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/μL and 1.09 copies/μL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities.

scholarly journals Recent Advancements in Enzyme-Based Lateral Flow Immunoassays

Sensors ◽  
2021 ◽  
Vol 21 (10) ◽  
pp. 3358
Author(s):  
Donato Calabria ◽  
Maria Maddalena Calabretta ◽  
Martina Zangheri ◽  
Elisa Marchegiani ◽  
Ilaria Trozzi ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Qualitative Information ◽  
Eye Color ◽  
Commercial Success ◽  
Color Changes ◽  
Naked Eye ◽  
Future Developments ◽  
Highly Sensitive ◽  
Critical Overview ◽  
Detection Technologies

Paper-based lateral-flow immunoassays (LFIAs) have achieved considerable commercial success and their impact in diagnostics is continuously growing. LFIA results are often obtained by visualizing by the naked eye color changes in given areas, providing a qualitative information about the presence/absence of the target analyte in the sample. However, this platform has the potential to provide ultrasensitive quantitative analysis for several applications. Indeed, LFIA is based on well-established immunological techniques, which have known in the last year great advances due to the combination of highly sensitive tracers, innovative signal amplification strategies and last-generation instrumental detectors. All these available progresses can be applied also to the LFIA platform by adapting them to a portable and miniaturized format. This possibility opens countless strategies for definitively turning the LFIA technique into an ultrasensitive quantitative method. Among the different proposals for achieving this goal, the use of enzyme-based immunoassay is very well known and widespread for routine analysis and it can represent a valid approach for improving LFIA performances. Several examples have been recently reported in literature exploiting enzymes properties and features for obtaining significative advances in this field. In this review, we aim to provide a critical overview of the recent progresses in highly sensitive LFIA detection technologies, involving the exploitation of enzyme-based amplification strategies. The features and applications of the technologies, along with future developments and challenges, are also discussed.

scholarly journals Intratumoral Canine Distemper Virus Infection Inhibits Tumor Growth by Modulation of the Tumor Microenvironment in a Murine Xenograft Model of Canine Histiocytic Sarcoma

2021 ◽  
Vol 22 (7) ◽  
pp. 3578
Author(s):  
Federico Armando ◽  
Adnan Fayyad ◽  
Stefanie Arms ◽  
Yvonne Barthel ◽  
Dirk Schaudien ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Virus Infection ◽  
Tumor Growth ◽  
Canine Distemper Virus ◽  
Xenograft Model ◽  
Histiocytic Sarcoma ◽  
Oncolytic Viruses ◽  
Canine Distemper ◽  
Murine Xenograft Model ◽  
The Impact

Histiocytic sarcomas refer to highly aggressive tumors with a poor prognosis that respond poorly to conventional treatment approaches. Oncolytic viruses, which have gained significant traction as a cancer therapy in recent decades, represent a promising option for treating histiocytic sarcomas through their replication and/or by modulating the tumor microenvironment. The live attenuated canine distemper virus (CDV) vaccine strain Onderstepoort represents an attractive candidate for oncolytic viral therapy. In the present study, oncolytic virotherapy with CDV was used to investigate the impact of this virus infection on tumor cell growth through direct oncolytic effects or by virus-mediated modulation of the tumor microenvironment with special emphasis on angiogenesis, expression of selected MMPs and TIMP-1 and tumor-associated macrophages in a murine xenograft model of canine histiocytic sarcoma. Treatment of mice with xenotransplanted canine histiocytic sarcomas using CDV induced overt retardation in tumor progression accompanied by necrosis of neoplastic cells, increased numbers of intratumoral macrophages, reduced angiogenesis and modulation of the expression of MMPs and TIMP-1. The present data suggest that CDV inhibits tumor growth in a multifactorial way, including direct cell lysis and reduction of angiogenesis and modulation of MMPs and their inhibitor TIMP-1, providing further support for the concept of its role in oncolytic therapies.

Sign in / Sign up

Export Citation Format

Share Document