scholarly journals Bioremoval of Cu2+ from CMP wastewater by a novel copper-resistant bacterium Cupriavidus gilardii CR3: characteristics and mechanisms

RSC Advances ◽  
2017 ◽  
Vol 7 (30) ◽  
pp. 18793-18802 ◽  
Author(s):  
Yushuang Yang ◽  
Mingzhong Hu ◽  
Dandan Zhou ◽  
Wei Fan ◽  
Xiaoyu Wang ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Metal Binding ◽  
Resistant Bacterium ◽  
Binding Capability

Bacteria of the genus Cupriavidus are known for the ability of resistance to various heavy metals and metal-binding capability.

Effect of Vitamin E and Selenium (Se) Supplementation on Biochemical Parameters and Expression of Metallothionein (MT-2) in Heavy Metals Exposed Buffaloes

2021 ◽  
Author(s):  
Himalaya Bhardwaj ◽  
Chanchal Singh ◽  
Shashi Nayyar ◽  
Sandeep Sodhi ◽  
Rajesh Jindal
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Vitamin E ◽  
Metal Binding ◽  
Biochemical Parameters ◽  
Lactic Dehydrogenase ◽  
Oral Feeding ◽  
Farm Animals ◽  
Blood Levels ◽  
Plasma Vitamin

Background: Farm animals may serve as bio-indicators of environmental pollution. Environmental heavy metals may disrupt the normal physiological and biochemical profile of the animals. The present study was planned to reduce the stress caused by heavy metal pollution by oral feeding of vitamin E and Selenium in heavy metals exposed buffaloes.Methods: Twenty buffaloes were selected on the basis of blood levels of heavy metals and divided into exposed and non-exposed groups. Exposed animals (n=10) were orally supplemented with 20 ml/day of Cargill E care Se® containing vitamin E, 100mg/ml and Se 0.5mg/ml for 30 days. Antioxidants, biochemical parameters and the expression of metallothionein-2 were analyzed after supplementation on 0, 15 and 30 days.Result: The levels of heavy metal were found to be elevated even after 30 days of supplementation. No significant alterations were observed in activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) after 30 days of supplementation. There was significantly (p less than 0.05) higher activity of blood glutathione and plasma Vitamin E. In supplemented group, plasma glucose, total cholesterol, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH), creatine kinase (CK) and total Immunoglobulin were found to be significantly reduced in supplemented group. Expression of metal binding protein, metallothionein-2 was found to be elevated in exposed animals despite supplementation with Vitamin E and Se for 30 days.

Effect of metallothionein 1A rs8052394 polymorphism on lead, cadmium, zinc, and aluminum levels in factory workers

2020 ◽  
Vol 36 (10) ◽  
pp. 816-822
Author(s):  
Preeti Singh ◽  
Prasenjit Mitra ◽  
Taru Goyal ◽  
Pilla VSN Kiran Kumar ◽  
Shailja Sharma ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Metal Binding ◽  
Genetic Effect ◽  
Atomic Absorption Spectrophotometry ◽  
Toxic Effects ◽  
Nucleotide Polymorphisms ◽  
Factory Workers ◽  
Genotypic Distribution ◽  
Study Population ◽  
Hardy Weinberg Equilibrium

Metallothioneins (MTs) are low molecular weight cysteine-rich, metal-binding proteins. They are involved in transportation and detoxification of heavy metals, homeostasis of essential metals, and as antioxidation against reactive oxygen species. Polymorphisms in a gene may increase or decrease the expression efficiency of a gene. This study aimed to determine the genetic effect of MT1A rs8052394 on lead (Pb), cadmium (Cd), zinc (Zn), and aluminum (Al) levels in factory workers. The study included 100 occupationally heavy metal exposed workers from different factories around Jodhpur. Pb, Cd, Zn, and Al levels were measured by atomic absorption spectrophotometry. Individuals with the GG genotype had lower Pb, Zn, and Al levels and higher Cd levels than AA and AG genotypes. The genotyping of MT1A rs8052394 was done by the polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP). The mean ± standard deviation of Pb, Cd, Zn, and Al was 5.88 ± 13.28 µg/dL, 3.52 ± 1.25 µg/L, 16.45 ± 16.69 µg/dL, and 58.92 ± 58.91 µg/L, respectively. A significant association was found between single-nucleotide polymorphisms (SNPs) of MT1A gene and Cd ( p = 0.006) and with Zn levels ( p = 0.031) but no association found with Pb and Al levels. Among the study population, 78 participants were homozygote major (AA), 19 were heterozygote (AG), and 3 were homozygote minor (GG). The χ 2 test presented the genotypic distribution of all three genotypes under the Hardy–Weinberg equilibrium ( p > 0.05). The frequency of the A allele was 87.5% (175) and the G allele was 12.5% (25). To conclude, polymorphism in rs8052394 of the MT1A gene is associated with increased metal accumulation which in turn may lead to increased toxic effects in the exposed individuals. Factory workers thus, should be more cautious about protecting their health against the toxic effects of heavy metals.

Multivariate analyses on heavy metal binding fractions of river sediments in Southern Taiwan

2000 ◽  
Vol 42 (7-8) ◽  
pp. 193-199 ◽  
Author(s):  
K. C. Yu ◽  
C. Y. Chang ◽  
L. J. Tsai ◽  
S. T. Ho
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Organic Matter ◽  
Metal Binding ◽  
River Sediments ◽  
Principal Component ◽  
Fe Oxides ◽  
Data Points ◽  
Southern Taiwan ◽  
Highly Correlated

This study depicts the amounts of heavy metals (Cu, Zn, Pb, Cr, Co, and Ni) bound to four geochemical compositions of sediments (carbonates, Mn oxides, Fe oxides, and organic matters), and the correlations between various geochemical compositions and their heavy-metal complexes. Hundreds of data, obtained from sediments of five main rivers (located in southern Taiwan), were analyzed by using multivariate analysis method. Among the four different geochemical compositions, the total amount of the six heavy metals bound to organic matter is the highest. Zn is easily bound to various geochemical compositions, especially carbonates in sediments of the Yenshui river and the Potzu river (i.e., the heavily heavy-metal polluted sediments); Cr, Pb, and Ni are mainly bound to both Fe oxides and organic matter; Cu has high affinity to organic matter. By performing principal component analyses, the data points of organic matter and both Pb and Cu associated with organic matter cluster together in sediments ofthe Peikang, the Potzu, and the Yenshui rivers, which indicates both Pb and Cu might be discharged from the same pollution sources in these rivers. Moreover, correlations between any two binding fractions of heavy metal associated with Fe oxides in different rivers are not consistent, which indicates some factors including the binding sites of Fe oxides, the extent of heavy metal pollution, binding competitions between heavy metals may affect the amounts of heavy metals bound to Fe oxides. Furthermore, it should be noted that the amount of Pb bound to Fe oxides is highly correlated with the amount of Fe oxides in sediments of the Peikang, the Potzu, and the Yenshui rivers.

Metal binding functions of metallothioneins in the slugArion vulgarisdiffer from metal-specific isoforms of terrestrial snails

Metallomics ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1638-1654 ◽  
Author(s):  
Martin Dvorak ◽  
Reinhard Lackner ◽  
Michael Niederwanger ◽  
Claire Rotondo ◽  
Raimund Schnegg ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Metal Binding ◽  
Arion Vulgaris ◽  
Terrestrial Snails

Arion vulgarisis a European slug with a huge potential for accumulating and detoxifying heavy metals.

scholarly journals Bioremoval of Different Heavy Metals in Industrial Effluent by the Resistant Fungal Strain Aspergillus niger

2021 ◽  
Vol 20 (4) ◽  
Author(s):  
K. J. Naveen Kumar ◽  
J. Prakash
Keyword(s):  
Heavy Metals ◽  
Aspergillus Niger ◽  
Metal Binding ◽  
Metal Removal ◽  
Low Cost ◽  
Heavy Metal Removal ◽  
Industrial Effluent ◽  
Toxic Metal ◽  
Differential Pulse ◽  
Toxic Heavy Metals

Developing countries are increasingly concerned with pollution due to toxic heavy metals in the environment. Unlike most organic pollutants which can be destroyed, toxic metal ions released into the environment often persist indefinitely circulating and eventually accumulating throughout the food chain thus posing a serious threat to mankind. The use of biological materials for heavy metal removal or recovery has gained importance in recent years due to their good performance and low cost. Among the various sources, both live and inactivated biomass of organisms exhibits interesting metal binding capacities. Their complex cell walls contain high content of functional groups like amino, amide, hydroxyl, carboxyl, and phosphate which have been implicated in metals binding. In the present study, Aspergillus niger was used to analyze the metal uptake from an aqueous solution. The determination of Cu+2, Pb+2, Cd+2, Zn+2, Co-2 and Ni+2 in samples was carried out by differential Pulse Anodic Voltammetry (DPASV) and the Voltammograms. Production of oxalic acid was carried out by submerged fermentation. The organism used in the present study has the ideal properties to sequester toxic metals and grow faster.

scholarly journals Modification of heavy metals toxicity by Cyanobacteria Nostoc sp. N27P72 and Nostoc sp. FB71 in Culture Conditions

2021 ◽  
Author(s):  
Elham Ghorbani ◽  
Bahareh Nowruzi ◽  
Mssomeh NejadAli ◽  
Azadeh Hekmat
Keyword(s):  
Heavy Metals ◽  
Metal Binding ◽  
Dry Weight ◽  
Culture Conditions ◽  
Control Culture ◽  
Media Culture ◽  
Gas Chromatography Mass Spectrometry ◽  
Benzenedicarboxylic Acid ◽  
The Media ◽  
Ft Ir

Abstract Purpose: Cyanobacteria are ecologically relevant prokaryotes that can be found in environments contaminated with heavy metals. As their photosynthetic machinery imposes high demands for metals, homeostasis of these micronutrients has been extensively considered in cyanobacteria. So far, most studies have focused on treatment of wastewaters using microalgae leads to remarkable reduction of an array of organic and inorganic nutrients, but what takes place in the extracellular environment when cells are exposed to external supplementation with heavy metals remains largely unknown. Methods: Here, extracellular polymeric substances (EPS) production in strains Nostoc sp. N27P72 and Nostoc sp. FB71 isolated from different habitats are reported and compared. Cultures of both strains, supplemented with either glucose, sucrose, lactose or maltose showed that production of EPS and cell dry weight was boosted by maltose supplementation. Result: Nostoc sp. N27P72 which was isolated from lime stones was higher, resulting in 9.1 ± 0.05 µg/ml and 1.01 ± 0.06 g/l in EPS and cell dry. The cell cultures tested for their ability to remove Cu(II), Cr(III) and Ni(II) in media culture containing the maltose and without maltose as control culture. Remarkably, we showed that although these elements can be toxic, supplementing the media culture can effectively sequester their toxic effects by increasing the production of EPSs, carbohydrates and total soluble proteins in comparison to control. The crude EPS showed metal adsorption capacity assuming the order Ni(II)> Cu(II)> Cr(III) from metal-binding experiments. Nickel was preferentially biosorbed with a maximal uptake of 188.8 ± 0.14 mg (g cell dry wt) -1 crude EPS. FT-IR spectroscopy revealed treatment with Ni made changes in the functional groups and glycoside linkages in both strains. Results of Gas Chromatography Mass Spectrometry (GC–MS) to determine the biochemical composition of Nostoc sp. N27P72 showed that strong Ni(II) removal capability is suspected to be associated with the high Cyclotrisiloxane and 1,2-Benzenedicarboxylic acid content. Conclusion: The results of these investigates specified that strains Nostoc sp. N27P72 is good candidates for the commercial production of EPS and might be utilized in bioremediation field as an alternative to synthetic and abiotic flocculants.

Engineering of Microbes for Heavy Metal Tolerance

2015 ◽  
pp. 73-91
Author(s):  
Megha D. Bhatt ◽  
Deepesh Bhatt
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Metal Binding ◽  
Surface Engineering ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Genetic Alterations ◽  
Molecular Tools ◽  
Genetic Level ◽  
Pros And Cons

Use of microorganisms and their enzymes to degrade heavy metal contaminants from the environment, is termed as bioremediation. This chapter majorly deals with heavy metals, their toxicity and their ill effects upon the environment. It depicts how microbes can help to combat the side effect of heavy metal toxicity by stimulating their natural defensive mechanism. In spite of their natural defensive system against metal pollution, still there is an urgent need of utilizing advanced molecular tools to further exaggerate their resistance ability for bioremediation. Earlier accumulation of heavy metals was done through overproduction of various metal binding proteins located in the cytoplasm. Recently cell surface engineering of microbes appears an attractive technology for removal or recovery of metal ions from the environment. To expedite the degradation of pollutant, a number of different molecular tools have been established for improving the microbial strains at molecular and genetic level. Microbial engineering thus, seems a promising approach which elucidates the effect of biotechnological processes used for decontaminating the polluted environment and in the future, humans and animals might gain from these organisms in remediating environmental contamination. However, these genetic modifications should be stable and harmless towards the nature as well as for the microbes itself and any genetic alterations must always ensure the actual pros and cons behind it.

Heavy metal-binding proteins from metal-stimulated bacteria as a novel adsorbent for metal removal technology

2006 ◽  
Vol 53 (6) ◽  
pp. 221-226 ◽  
Author(s):  
D. Sano ◽  
K. Myojo ◽  
T. Omura
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Activated Sludge ◽  
Metal Binding ◽  
Binding Proteins ◽  
Metal Removal ◽  
Heavy Metal Removal ◽  
Copper Ion ◽  
Amino Acid Sequences ◽  
Heavy Metal Binding

Water pollution with toxic heavy metals is of growing concern because heavy metals could bring about serious problems for not only ecosystems in the water environment but also human health. Some metal removal technologies have been in practical use, but much energy and troublesome treatments for chemical wastes are required to operate these conventional technologies. In this study, heavy metal-binding proteins (HMBPs) were obtained from metal-stimulated activated sludge culture with affinity chromatography using copper ion as a ligand. Two-dimensional electrophoresis revealed that a number of proteins in activated sludge culture were recovered as HMBPs for copper ion. N-termini of five HMBPs were determined, and two of them were found to be newly discovered proteins for which no amino acid sequences in protein databases were retrieved at more than 80% identities. Metal-coordinating amino acids occupied 38% of residues in one of the N-terminal sequences of the newly discovered HMBPs. Since these HMBPs were expected to be stable under conditions of water and wastewater treatments, it would be possible to utilize HMBPs as novel adsorbents for heavy metal removal if mass volume of HMBPs can be obtained with protein cloning techniques.

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