Control of electrostatic interaction between a molecular beacon aptamer and conjugated polyelectrolyte for detection range-tunable ATP assay

2017 ◽  
Vol 8 (40) ◽  
pp. 6329-6334 ◽  
Author(s):  
J.-E. Jeong ◽  
H. Y. Woo
Keyword(s):  
Electrostatic Interaction ◽  
Molecular Beacon ◽  
Detection Range ◽  
Conjugated Polyelectrolyte ◽  
Atp Assay ◽  
New Strategy ◽  
Ionic Density ◽  
Fine Tune

A new strategy is suggested to fine-tune the detection range by controlling the ionic density of CPEs in the MBA/CPE-based ATP assay.

scholarly journals Principal factors that determine the extension of detection range in molecular beacon aptamer/conjugated polyelectrolyte bioassays

2015 ◽  
Vol 6 (3) ◽  
pp. 1887-1894 ◽  
Author(s):  
Ji-Eun Jeong ◽  
Boram Kim ◽  
Shinjae Woo ◽  
Sungu Hwang ◽  
Guillermo C. Bazan ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Limit Of Detection ◽  
Fine Tuning ◽  
Detection Range ◽  
Conjugated Polyelectrolyte ◽  
Principal Factors ◽  
New Bioassay

A new bioassay strategy based on the molecular beacon aptamer/conjugated polyelectrolyte demonstrates a fine-tuning of the detection range and limit of detection for weakly-binding targets.

Surface decoration of ZnO nanoparticles: A new strategy to fine tune the recognition properties of imine linked receptor

2012 ◽  
Vol 166-167 ◽  
pp. 467-472 ◽  
Author(s):  
Hemant Sharma ◽  
Navneet Kaur ◽  
Thangarasu Pandiyan ◽  
Narinder Singh
Keyword(s):  
Zno Nanoparticles ◽  
New Strategy ◽  
Surface Decoration ◽  
Fine Tune

Cationic Conjugated Polyelectrolyte/Molecular Beacon Complex for Sensitive, Sequence-Specific, Real-Time DNA Detection

Langmuir ◽  
2008 ◽  
Vol 24 (21) ◽  
pp. 12138-12141 ◽  
Author(s):  
Xuli Feng ◽  
Xinrui Duan ◽  
Libin Liu ◽  
Lingling An ◽  
Fude Feng ◽  
...  
Keyword(s):  
Real Time ◽  
Molecular Beacon ◽  
Dna Detection ◽  
Conjugated Polyelectrolyte

A novel kinase-based ATP assay using molecular beacon

2008 ◽  
Vol 372 (1) ◽  
pp. 131-133 ◽  
Author(s):  
Changbei Ma ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
Zhiwen Tang ◽  
Wei Li ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Atp Assay

scholarly journals Aggregation of Gold Nanoparticles Caused in Two Different Ways Involved in 4-Mercaptophenylboronic Acidand Hydrogen Peroxide

Materials ◽  
2019 ◽  
Vol 12 (11) ◽  
pp. 1802
Author(s):  
Runmei Li ◽  
Xuefan Gu ◽  
Xingtang Liang ◽  
Shi Hou ◽  
Daodao Hu
Keyword(s):  
Hydrogen Peroxide ◽  
Boric Acid ◽  
Color Change ◽  
H2o2 Concentration ◽  
Ph Change ◽  
Detection Range ◽  
Color Changes ◽  
New Strategy ◽  
Colorimetric Assays ◽  
The Difference

The difference in gold nanoparticle (AuNPs) aggregation caused by different mixing orders of AuNPs, 4-mercaptophenylboronic acid (4-MPBA), and hydrogen peroxide (H2O2) has been scarcely reported. We have found that the color change of a ((4-MPBA + AuNPs) + H2O2) mixture caused by H2O2 is more sensitive than that of a ((4-MPBA + H2O2) + AuNPs) mixture. For the former mixture, the color changes obviously with H2O2 concentrations in the range of 0~0.025%. However, for the latter mixture, the corresponding H2O2 concentration is in the range of 0~1.93%. The mechanisms on the color change originating from the aggregation of AuNPs occurring in the two mixtures were investigated in detail. For the ((4-MPBA + H2O2) + AuNPs) mixture, free 4-MPBA is oxidized by H2O2 to form bis(4-hydroxyphenyl) disulfide (BHPD) and peroxoboric acid. However, for the ((4-MPBA+AuNPs) + H2O2) mixture, immobilized 4-MPBA is oxidized by H2O2 to form 4-hydroxythiophenol (4-HTP) and boric acid. The decrease in charge on the surface of AuNPs caused by BHPD, which has alarger steric hindrance, is poorer than that caused by -4-HTP, and this is mainly responsible for the difference in the aggregation of AuNPs in the two mixtures. The formation of boric acid and peroxoboric acid in the reaction between 4-MPBA and H2O2 can alter the pH of the medium, and the effect of the pH change on the aggregation of AuNPs should not be ignored. These findings not only offer a new strategy in colorimetric assays to expand the detection range of hydrogen peroxide concentrations but also assist in deepening the understanding of the aggregation of citrate-capped AuNPs involved in 4-MPBA and H2O2, as well as in developing other probes.

A novel sensitive and selective ligation-based ATP assay using a molecular beacon

The Analyst ◽  
2013 ◽  
Vol 138 (10) ◽  
pp. 3013 ◽  
Author(s):  
Changbei Ma ◽  
Zhiwen Tang ◽  
Kemin Wang ◽  
Xiaohai Yang ◽  
Weihong Tan
Keyword(s):  
Molecular Beacon ◽  
Atp Assay

Conjugated Polyelectrolyte-Based New Strategy for in Situ Detection of Carbon Dioxide

2017 ◽  
Vol 9 (24) ◽  
pp. 20313-20317 ◽  
Author(s):  
Yibing Fan ◽  
Chengfen Xing ◽  
Hongbo Yuan ◽  
Ran Chai ◽  
Linfei Zhao ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Conjugated Polyelectrolyte ◽  
New Strategy ◽  
In Situ Detection
Sign in / Sign up

Export Citation Format

Share Document