A novel sensitive and selective ligation-based ATP assay using a molecular beacon

The Analyst ◽  
2013 ◽  
Vol 138 (10) ◽  
pp. 3013 ◽  
Author(s):  
Changbei Ma ◽  
Zhiwen Tang ◽  
Kemin Wang ◽  
Xiaohai Yang ◽  
Weihong Tan
Keyword(s):  
Molecular Beacon ◽  
Atp Assay

A novel kinase-based ATP assay using molecular beacon

2008 ◽  
Vol 372 (1) ◽  
pp. 131-133 ◽  
Author(s):  
Changbei Ma ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
Zhiwen Tang ◽  
Wei Li ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Atp Assay

Control of electrostatic interaction between a molecular beacon aptamer and conjugated polyelectrolyte for detection range-tunable ATP assay

2017 ◽  
Vol 8 (40) ◽  
pp. 6329-6334 ◽  
Author(s):  
J.-E. Jeong ◽  
H. Y. Woo
Keyword(s):  
Electrostatic Interaction ◽  
Molecular Beacon ◽  
Detection Range ◽  
Conjugated Polyelectrolyte ◽  
Atp Assay ◽  
New Strategy ◽  
Ionic Density ◽  
Fine Tune

A new strategy is suggested to fine-tune the detection range by controlling the ionic density of CPEs in the MBA/CPE-based ATP assay.

scholarly journals Single Step In Situ Detection of Surface Protein and MicroRNA in Clustered Extracellular Vesicles Using Flow Cytometry

2021 ◽  
Vol 10 (2) ◽  
pp. 319
Author(s):  
Hee Cheol Yang ◽  
Won Jong Rhee
Keyword(s):  
Extracellular Vesicles ◽  
Liquid Biopsy ◽  
Molecular Beacon ◽  
Disease Diagnosis ◽  
Single Step ◽  
Flow Cytometer ◽  
Surface Proteins ◽  
Biomarker Detection ◽  
In Situ Detection

Because cancers are heterogeneous, it is evident that multiplexed detection is required to achieve disease diagnosis with high accuracy and specificity. Extracellular vesicles (EVs) have been a subject of great interest as sources of novel biomarkers for cancer liquid biopsy. However, EVs are nano-sized particles that are difficult to handle; thus, it is necessary to develop a method that enables efficient and straightforward EV biomarker detection. In the present study, we developed a method for single step in situ detection of EV surface proteins and inner miRNAs simultaneously using a flow cytometer. CD63 antibody and molecular beacon-21 were investigated for multiplexed biomarker detection in normal and cancer EVs. A phospholipid-polymer-phospholipid conjugate was introduced to induce clustering of the EVs analyzed using nanoparticle tracking analysis, which enhanced the detection signals. As a result, the method could detect and distinguish cancer cell-derived EVs using a flow cytometer. Thus, single step in situ detection of multiple EV biomarkers using a flow cytometer can be applied as a simple, labor- and time-saving, non-invasive liquid biopsy for the diagnosis of various diseases, including cancer.

scholarly journals Effectiveness of photon-initiated photoacoustic streaming in root canal models with different diameters or tapers

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Cheng Wen ◽  
Yuanyuan Kong ◽  
Jian Zhao ◽  
Yang Li ◽  
Ya Shen ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Root Canal ◽  
Distilled Water ◽  
Canal Irrigation ◽  
Root Canals ◽  
Atp Assay ◽  
Root Canal Irrigation ◽  
Average Fluorescence Intensity ◽  
Different Diameters ◽  
Average Fluorescence

Abstract Background This study aimed to compare the use of photon-initiated photoacoustic streaming (PIPS) and conventional needle irrigation (CNI) in conjunction with different concentrations of sodium hypochlorite (NaOCl) to remove Enterococcus faecalis (E. faecalis) suspended bacteria and biofilms from root canal systems with different diameters or tapers. Methods Artificial root canal samples (n = 480) were randomly divided into three groups (n = 160/group). The canals were prepared to fit file sizes #10/.02, #25/.02, or #25/.06. The size #10/.02 group was incubated for seven days. The size #25/.02 or #25/.06 group was incubated for 2 days. A stable biological model of E. faecalis infection was established. The root canals were washed with distilled water or with 1%, 2%, or 5.25% NaOCl combined with CNI or PIPS. Bacterial suspensions and biofilms were assessed using an ATP assay kit and fluorescence microscopy. Image-Pro Plus was used to analyse the average fluorescence intensity to determine the most suitable root canal irrigation solution. Results In the CNI and PIPS groups, the ATP value of the 5.25% NaOCl subgroup was the lowest, followed by that of the 2% and 1% NaOCl subgroups. The ATP value of the distilled water subgroup was the highest (P < 0.05). When the root canal taper was 0.02, the ATP value of the #10/.02 + PIPS group was significantly lower than that of the #25/.02 + CNI group (P < 0.05). The average fluorescence intensity of the #10/.02 + PIPS group was lower than that of the #25/.02 + CNI group (P < 0.05). When the apical diameter was #25, the ATP value of the 0.02 taper in the PIPS group was lower than that of the 0.06 taper in the CNI group (P < 0.05), and the average fluorescence intensity of the 0.02 taper + PIPS group was lower than that of the 0.06 taper + CNI group (P < 0.05). PIPS combined with 2% and 5.25% NaOCl effectively improved the long-term antibacterial effect after irrigation and re-culture for 6 h. Conclusions Compared with CNI, PIPS has greater ability to remove bacteria in root canals with a small preparation diameter and a small taper. PIPS with 2% and 5.25% NaOCl exhibited superior antibacterial and bacteriostatic effects.

scholarly journals Novel nucleic acid origami structures and conventional molecular beacon–based platforms: a comparison in biosensing applications

2021 ◽  
Author(s):  
Noemi Bellassai ◽  
Roberta D’Agata ◽  
Giuseppe Spoto
Keyword(s):  
Nucleic Acid ◽  
Structural Properties ◽  
Molecular Beacon ◽  
Dna Origami ◽  
Molecular Beacons ◽  
Conformational Polymorphism ◽  
Functional Systems ◽  
Similarities And Differences ◽  
Nucleic Acid Structures ◽  
Nucleic Acid Sequences

AbstractNucleic acid nanotechnology designs and develops synthetic nucleic acid strands to fabricate nanosized functional systems. Structural properties and the conformational polymorphism of nucleic acid sequences are inherent characteristics that make nucleic acid nanostructures attractive systems in biosensing. This review critically discusses recent advances in biosensing derived from molecular beacon and DNA origami structures. Molecular beacons belong to a conventional class of nucleic acid structures used in biosensing, whereas DNA origami nanostructures are fabricated by fully exploiting possibilities offered by nucleic acid nanotechnology. We present nucleic acid scaffolds divided into conventional hairpin molecular beacons and DNA origami, and discuss some relevant examples by focusing on peculiar aspects exploited in biosensing applications. We also critically evaluate analytical uses of the synthetic nucleic acid structures in biosensing to point out similarities and differences between traditional hairpin nucleic acid sequences and DNA origami. Graphical abstract

scholarly journals Fluorogenic Aptasensors with Small Molecules

Chemosensors ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 54
Author(s):  
Eun-Song Lee ◽  
Jeong Min Lee ◽  
Hea-Jin Kim ◽  
Young-Pil Kim
Keyword(s):  
Small Molecules ◽  
In Vitro Selection ◽  
Molecular Beacon ◽  
Future Directions ◽  
Rna Molecules ◽  
Amplification Process ◽  
Target Molecules ◽  
And Performance ◽  
Fluorescence Turn On

Aptamers are single-stranded DNA or RNA molecules that can be identified through an iterative in vitro selection–amplification process. Among them, fluorogenic aptamers in response to small molecules have been of great interest in biosensing and bioimaging due to their rapid fluorescence turn-on signals with high target specificity and low background noise. In this review, we report recent advances in fluorogenic aptasensors and their applications to in vitro diagnosis and cellular imaging. These aptasensors modulated by small molecules have been implemented in different modalities that include duplex or molecular beacon-type aptasensors, aptazymes, and fluorogen-activating aptamer reporters. We highlight the working principles, target molecules, modifications, and performance characteristics of fluorogenic aptasensors, and discuss their potential roles in the field of biosensor and bioimaging with future directions and challenges.

scholarly journals Use of a molecular beacon based fluorescent method for assaying uracil DNA glycosylase (Ung) activity and inhibitor screening

2021 ◽  
Vol 26 ◽  
pp. 100954
Author(s):  
Avani Mehta ◽  
Prateek Raj ◽  
Sandeep Sundriyal ◽  
Balasubramanian Gopal ◽  
Umesh Varshney
Keyword(s):  
Molecular Beacon ◽  
Dna Glycosylase ◽  
Uracil Dna Glycosylase ◽  
Inhibitor Screening ◽  
Fluorescent Method

Covalently Mercurated Molecular Beacon for Discriminating the Canonical Nucleobases

ChemBioChem ◽  
2020 ◽  
Author(s):  
Asmo Aro‐Heinilä ◽  
Tuomas Lönnberg ◽  
Pasi Virta
Keyword(s):  
Molecular Beacon
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