scholarly journals Phenothiazine-linked nucleosides and nucleotides for redox labelling of DNA

2017 ◽  
Vol 15 (33) ◽  
pp. 6984-6996 ◽  
Author(s):  
Anna Simonova ◽  
Luděk Havran ◽  
Radek Pohl ◽  
Miroslav Fojta ◽  
Michal Hocek
Keyword(s):  
Electrochemical Detection ◽  
Enzymatic Synthesis ◽  
Dna Bases ◽  
Modified Nucleotides

New phenothiazine-modified nucleotides were prepared and used for enzymatic synthesis of labelled DNA for electrochemical detection and redox coding of DNA bases.

scholarly journals Measurement of oxidized and methylated DNA bases by HPLC with electrochemical detection

1996 ◽  
Vol 318 (1) ◽  
pp. 21-23 ◽  
Author(s):  
Harparkash KAUR ◽  
Barry HALLIWELL
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Dna Damage ◽  
Electrochemical Detection ◽  
Oxidative Dna Damage ◽  
The Other ◽  
Dna Bases ◽  
Cancer Development ◽  
Methylated Dna ◽  
Important Contributor

Oxidative DNA damage is thought to be an important contributor to cancer development and to be affected by dietary constituents, so its accurate measurement is important. DNA methylation is recognized as an important mechanism affecting gene expression. In the present paper we describe an HPLC-with-electrochemical-detection procedure that allows rapid and sensitive measurement of four oxidized (2,6-diamino-4-hydroxy-5-formamidopyrimidine, 5-hydroxyuracil, 8-hydroxyguanine, 8-hydroxyadenine) and three methylated (7-methylguanine, 1-methylguanine, O6-methylguanine) bases in acid hydrolysates of DNA. Guanine was also detected, but was clearly separated from the other bases.

Use of DNA Repair Enzymes in Electrochemical Detection of Damage to DNA Bases in Vitro and in Cells

2005 ◽  
Vol 77 (9) ◽  
pp. 2920-2927 ◽  
Author(s):  
Kateřina Cahová-Kuchaříková ◽  
Miroslav Fojta ◽  
Tomáš Mozga ◽  
Emil Paleček
Keyword(s):  
Dna Repair ◽  
Electrochemical Detection ◽  
Dna Bases ◽  
Dna Repair Enzymes ◽  
Repair Enzymes ◽  
In Cells

scholarly journals Enzymatic synthesis of gene-length single-stranded DNA

2017 ◽  
Author(s):  
Rémi Veneziano ◽  
Tyson R. Shepherd ◽  
Sakul Ratanalert ◽  
Leila Bellou ◽  
Chaoqun Tao ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Nucleotide Composition ◽  
Maximum Length ◽  
Gene Length ◽  
Modified Nucleotides ◽  
Asymmetric Pcr ◽  
Single Stranded Dna ◽  
Well Control ◽  
Homology Directed Repair

Single-stranded DNA (ssDNA) increases the likelihood of homology directed repair with reduced cellular toxic ity, yet ssDNA synthesis strategies are limited by the maximum length attainable, as well control over nucleotide composition. Here, we apply purely enzymatic synthesis to generate ssDNA greater than 15 kb using asymmetric PCR, and illustrate the incorporation of diverse modified nucleotides for therapeutic and imaging applications.

A copper-based metal–organic framework/graphene nanocomposite for the sensitive and stable electrochemical detection of DNA bases

The Analyst ◽  
2020 ◽  
Vol 145 (5) ◽  
pp. 1933-1942 ◽  
Author(s):  
Xiuyun Wang ◽  
Jie Zhang ◽  
Yuanan Wei ◽  
Tianyu Xing ◽  
Tingting Cao ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Metal Organic Framework ◽  
Dna Bases ◽  
Electrode Modification ◽  
Metal Organic ◽  
Guanine And Adenine ◽  
Graphene Nanocomposite ◽  
Easy Operation ◽  
Organic Framework

A simple and easy-operation electrode modification strategy was proposed using Cu-MOF/GO nanohybrids for physiologists and pathologists for the feasible and reliable simultaneous electrochemical detections of DNA bases, namely guanine and adenine.

Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases

2013 ◽  
Vol 19 (38) ◽  
pp. 12720-12731 ◽  
Author(s):  
Jana Balintová ◽  
Medard Plucnara ◽  
Pavlína Vidláková ◽  
Radek Pohl ◽  
Luděk Havran ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Dna Bases ◽  
Redox Label
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