Cobalamin-dependent radicalS-adenosyl-l-methionine enzymes in natural product biosynthesis

2018 ◽  
Vol 35 (8) ◽  
pp. 707-720 ◽  
Author(s):  
Susan C. Wang
Keyword(s):  
Natural Product ◽  
Biosynthetic Pathways ◽  
Natural Product Biosynthesis

This highlight examines the functions of cobalamin-dependent radicalS-adenosyl-l-methionine enzymes that catalyse chemically-challenging reactions in several bacterial natural product biosynthetic pathways.

Genome-Guided Discovery of Natural Products and Biosynthetic Pathways from Australia’s Untapped Microbial Megadiversity

2016 ◽  
Vol 69 (2) ◽  
pp. 129 ◽  
Author(s):  
John A. Kalaitzis ◽  
Shane D. Ingrey ◽  
Rocky Chau ◽  
Yvette Simon ◽  
Brett A. Neilan
Keyword(s):  
Natural Products ◽  
Natural Product ◽  
Genome Sequencing ◽  
Dna Sequences ◽  
Gene Clusters ◽  
Biosynthetic Pathways ◽  
Natural Product Biosynthesis ◽  
Guided Discovery ◽  
Biosynthesis Gene ◽  
Microbial Natural Products

Historically microbial natural product biosynthesis pathways were elucidated mainly by isotope labelled precursor directed feeding studies. Now the genetics underpinning the assembly of microbial natural products biosynthesis is so well understood that some pathways and their products can be predicted from DNA sequences alone. The association between microbial natural products and their biosynthesis gene clusters is now driving the field of ‘genetics guided natural product discovery’. This account overviews our research into cyanotoxin biosynthesis before the genome sequencing era through to some recent discoveries resulting from the mining of Australian biota for natural product biosynthesis pathways.

New chemistry from natural product biosynthesis

2016 ◽  
Vol 44 (3) ◽  
pp. 738-744 ◽  
Author(s):  
Catherine B. Hubert ◽  
Sarah M. Barry
Keyword(s):  
Natural Product ◽  
Chemical Reactions ◽  
Bond Activation ◽  
Synthetic Chemistry ◽  
Biosynthetic Pathways ◽  
Natural Product Biosynthesis ◽  
Bulk Chemicals ◽  
Enzyme Discovery ◽  
Starting Point ◽  
Product Assembly

Catalysts are a vital part of synthetic chemistry. However, there are still many important reactions for which catalysts have not been developed. The use of enzymes as biocatalysts for synthetic chemistry is growing in importance due to the drive towards sustainable methods for producing both bulk chemicals and high value compounds such as pharmaceuticals, and due to the ability of enzymes to catalyse chemical reactions with excellent stereoselectivity and regioselectivity. Such challenging transformations are a common feature of natural product biosynthetic pathways. In this mini-review, we discuss the potential to use biosynthetic pathways as a starting point for biocatalyst discovery. We introduce the reader to natural product assembly and tailoring, then focus on four classes of enzyme that catalyse C─H bond activation reactions to functionalize biosynthetic precursors. Finally, we briefly discuss the challenges involved in novel enzyme discovery.

scholarly journals Recent advances in the elucidation of enzymatic function in natural product biosynthesis

F1000Research ◽  
2016 ◽  
Vol 4 ◽  
pp. 1399 ◽  
Author(s):  
Gao-Yi Tan ◽  
Zixin Deng ◽  
Tiangang Liu
Keyword(s):  
Natural Product ◽  
New Drugs ◽  
Biosynthetic Pathways ◽  
Natural Product Biosynthesis ◽  
Heterologous Biosynthesis ◽  
Artemisinic Acid ◽  
The Past ◽  
Enzymatic Function ◽  
Active Natural ◽  
Successful Production

With the successful production of artemisinic acid in yeast, the promising potential of synthetic biology for natural product biosynthesis is now being realized. The recent total biosynthesis of opioids in microbes is considered to be another landmark in this field. The importance and significance of enzymes in natural product biosynthetic pathways have been re-emphasized by these advancements. Therefore, the characterization and elucidation of enzymatic function in natural product biosynthesis are undoubtedly fundamental for the development of new drugs and the heterologous biosynthesis of active natural products. Here, discoveries regarding enzymatic function in natural product biosynthesis over the past year are briefly reviewed.

scholarly journals Recent advances in the elucidation of enzymatic function in natural product biosynthesis

F1000Research ◽  
2015 ◽  
Vol 4 ◽  
pp. 1399 ◽  
Author(s):  
Gao-Yi Tan ◽  
Zixin Deng ◽  
Tiangang Liu
Keyword(s):  
Natural Product ◽  
New Drugs ◽  
Biosynthetic Pathways ◽  
Natural Product Biosynthesis ◽  
Heterologous Biosynthesis ◽  
Artemisinic Acid ◽  
The Past ◽  
Enzymatic Function ◽  
Active Natural ◽  
Successful Production

With the successful production of artemisinic acid in yeast, the promising potential of synthetic biology for natural product biosynthesis is now being realized. The recent total biosynthesis of opioids in microbes is considered to be another landmark in this field. The importance and significance of enzymes in natural product biosynthetic pathways have been re-emphasized by these advancements. Therefore, the characterization and elucidation of enzymatic function in natural product biosynthesis are undoubtedly fundamental for the development of new drugs and the heterologous biosynthesis of active natural products. Here, discoveries regarding enzymatic function in natural product biosynthesis over the past year are briefly reviewed.

Structural and Functional Analyses of a Spiro-Carbon-Forming, Highly Promiscuous Epoxidase from Fungal Natural Product Biosynthesis

Biochemistry ◽  
2020 ◽  
Vol 59 (51) ◽  
pp. 4787-4792
Author(s):  
Takuma Matsushita ◽  
Shinji Kishimoto ◽  
Kodai Hara ◽  
Hiroshi Hashimoto ◽  
Kenji Watanabe
Keyword(s):  
Natural Product ◽  
Natural Product Biosynthesis ◽  
Functional Analyses

Beyond peptide bond formation: the versatile role of condensation domains in natural product biosynthesis

2021 ◽  
Author(s):  
Sofie Dekimpe ◽  
Joleen Masschelein
Keyword(s):  
Natural Product ◽  
Bond Formation ◽  
Peptide Bond ◽  
Chemical Diversity ◽  
Peptide Bond Formation ◽  
Natural Product Biosynthesis

Condensation domains perform highly diverse functions during natural product biosynthesis and are capable of generating remarkable chemical diversity.

Mind the mushroom: natural product biosynthetic genes and enzymes of Basidiomycota

2021 ◽  
Author(s):  
Markus Gressler ◽  
Nikolai A. Löhr ◽  
Tim Schäfer ◽  
Stefanie Lawrinowitz ◽  
Paula Sophie Seibold ◽  
...  
Keyword(s):  
Natural Product ◽  
Biosynthetic Genes ◽  
Natural Product Biosynthesis

This article comprehensively reviews basidiomycete enzymes and their genes involved in natural product biosynthesis and primarily focuses on typical pathways and enzymes, along with the methods applied to investigate mushroom metabolism.

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