scholarly journals Peptide-fluorophore/AuNP conjugate-based two-photon excited fluorescent nanosensor for caspase-3 activity imaging assay in living cells and tissue

MedChemComm ◽  
2017 ◽  
Vol 8 (7) ◽  
pp. 1435-1439 ◽  
Author(s):  
Qingshan Ge ◽  
Ningning Wang ◽  
Jishan Li ◽  
Ronghua Yang
Keyword(s):  
Rat Liver ◽  
Liver Tissue ◽  
Caspase 3 ◽  
Living Cells ◽  
Live Cells ◽  
Tissue Slices ◽  
Two Photon

Via the assembly of two-photon dye (TPdye)-labeled peptides on the gold nanoparticle's surface, a novel two-photon excited (TPE) fluorescent nanosensor has been developed for the measurement of caspase-3 activity in live cells and rat liver tissue slices.

A two-photon fluorescent probe for bio-imaging of formaldehyde in living cells and tissues

The Analyst ◽  
2016 ◽  
Vol 141 (11) ◽  
pp. 3395-3402 ◽  
Author(s):  
Jun-Bin Li ◽  
Qian-Qian Wang ◽  
Lin Yuan ◽  
Yong-Xiang Wu ◽  
Xiao-Xiao Hu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Live Cells ◽  
Two Photon ◽  
Bio Imaging

We report a new two-photon fluorescent probe FATP1 that allows for the two-photon bio-imaging of formaldehyde in live cells and tissues.

Poly β-Cyclodextrin/TPdye Nanomicelle-based Two-Photon Nanoprobe for Caspase-3 Activation Imaging in Live Cells and Tissues

2014 ◽  
Vol 86 (22) ◽  
pp. 11440-11450 ◽  
Author(s):  
Huijuan Yan ◽  
Leiliang He ◽  
Wenjie Zhao ◽  
Jishan Li ◽  
Yue Xiao ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Live Cells ◽  
Two Photon

scholarly journals An Immunomodulatory Protein (Ling Zhi-8) from aGanoderma lucidumInduced Acceleration of Wound Healing in Rat Liver Tissues after Monopolar Electrosurgery

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Hao-Jan Lin ◽  
Yushan-Sophie Chang ◽  
Li-Hsiang Lin ◽  
Chiung-Fang Haung ◽  
Chia-Yu Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Western Blot ◽  
Rat Liver ◽  
Blot Analysis ◽  
Liver Tissue ◽  
Caspase 3 ◽  
Dapi Staining ◽  
Monopolar Electrosurgery ◽  
Immunomodulatory Protein ◽  
Liver Tissues

The purpose of this study was to investigate the effect of an immunomodulatory protein (Ling Zhi-8, LZ-8) on wound healing in rat liver tissues after monopolar electrosurgery. Animals were sacrificed for evaluations at 0, 3, 7, and 28 days postoperatively. It was found that the wound with the LZ-8 treatment significantly increases wound healing. Western blot analysis clearly indicated that the expression of NF-κB was decreased at 3, 7, and 28 days when liver tissues were treated with LZ-8. Moreover, caspase-3 activity of the liver tissue also significantly decreases at 7 and 28 days, respectively. DAPI staining and TUNEL assays revealed that only a minimal dispersion of NF-κB was found on the liver tissue treated with LZ-8 at day 7 as compared with day 3 and tissues without LZ-8 treatment. Similarly, apoptosis was decreased on liver tissues treated with LZ-8 at 7 days when compared to the control (monopolar electrosurgery) tissues. Therefore, the analytical results demonstrated that LZ-8 induced acceleration of wound healing in rat liver tissues after monopolar electrosurgery.

A near-infrared water-soluble fluorescent probe for the detection of biothiols in living cells and Escherichia coli

2019 ◽  
Vol 11 (6) ◽  
pp. 821-826 ◽  
Author(s):  
Ling-Ling Li ◽  
Pei-Yang He ◽  
Lei Shi ◽  
Sheng-Lin Pan ◽  
Meng-Yang Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fluorescent Probe ◽  
Rat Liver ◽  
Liver Tissue ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Living Cells ◽  
Water Soluble ◽  
Imaging Method ◽  
Selective Detection

An efficient near-infrared imaging method for the selective detection of biothiols in living cells, Escherichia coli and rat liver tissue is developed using an off-on fluorescent probe.

scholarly journals Discovery of catalytic-site-fluorescent probes for tracing phosphodiesterase 5 in living cells

RSC Advances ◽  
2021 ◽  
Vol 11 (51) ◽  
pp. 31967-31971
Author(s):  
Meiying Qiu ◽  
Deyan Wu ◽  
Yi-You Huang ◽  
Yue Huang ◽  
Qian Zhou ◽  
...  
Keyword(s):  
Drug Screening ◽  
Fluorescent Probes ◽  
Drug Target ◽  
Living Cells ◽  
Catalytic Site ◽  
Live Cells ◽  
Tissue Slices ◽  
Phosphodiesterase 5

To enhance the understanding of PDE5 as the drug target. Herein, we designed catalytic-site-fluorescent probes that can be applied to PDE5 visualization in live cells and tissue slices, implying the potential in diagnosis and drug screening.

Selective and Real-Time Detection of Nitric Oxide by a Two-Photon Fluorescent Probe in Live Cells and Tissue Slices

2017 ◽  
Vol 89 (19) ◽  
pp. 10511-10519 ◽  
Author(s):  
Chun-Guang Dai ◽  
Ji-Long Wang ◽  
Ying-Long Fu ◽  
Hong-Ping Zhou ◽  
Qin-Hua Song
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Fluorescent Probe ◽  
Live Cells ◽  
Tissue Slices ◽  
Two Photon ◽  
Real Time Detection

A facile two-photon fluorescent probe: an endoplasmic reticulum tracker monitoring ER stress and vesicular transport to lysosomes

2019 ◽  
Vol 55 (3) ◽  
pp. 294-297 ◽  
Author(s):  
Pratibha Kumari ◽  
Sanjay K. Verma ◽  
Shaikh M. Mobin
Keyword(s):  
Endoplasmic Reticulum ◽  
Er Stress ◽  
Fluorescent Probe ◽  
Vesicular Transport ◽  
Living Cells ◽  
Vesicle Transport ◽  
Live Cells ◽  
Tumor Spheroids ◽  
Dynamic Changes ◽  
Two Photon

The two-photon fluorescent organic probe ERLp selectively labels the endoplasmic reticulum in live cells and tumor spheroids. ERLp can also monitor ER dynamic changes during ER stress and vesicle transport from the ER to the lysosome in living cells.

Measurement of Water Transport During Freezing in Mammalian Liver Tissue: Part II—The Use of Differential Scanning Calorimetry

1998 ◽  
Vol 120 (5) ◽  
pp. 559-569 ◽  
Author(s):  
R. V. Devireddy ◽  
J. C. Bischof
Keyword(s):  
Water Transport ◽  
Rat Liver ◽  
Liver Tissue ◽  
Freeze Substitution ◽  
Sprague Dawley ◽  
Transport Parameters ◽  
Biophysical Parameters ◽  
Scanning Calorimetry ◽  
Tissue Slices ◽  
Low Temperature Microscopy

There is currently a need for experimental techniques to assay the biophysical response (water transport or intracellular ice formation, IIF) during freezing in the cells of whole tissue slices. These data are important in understanding and optimizing biomedical applications of freezing, particularly in cryosurgery. This study presents a new technique using a Differential Scanning Calorimeter (DSC) to obtain dynamic and quantitative water transport data in whole tissue slices during freezing. Sprague-Dawley rat liver tissue was chosen as our model system. The DSC was used to monitor quantitatively the heat released by water transported from the unfrozen cell cytoplasm to the partially frozen vascular/extracellular space at 5°C/min. This technique was previously described for use in a single cell suspension system (Devireddy, et al. 1998). A model of water transport was fit to the DSC data using a nonlinear regression curve-fitting technique, which assumes that the rat liver tissue behaves as a two-compartment Krogh cylinder model. The biophysical parameters of water transport for rat liver tissue at 5°C/min were obtained as Lpg = 3.16 x 10−13 m3/Ns (1.9 μm/min-atm), ELp = 265 kJ/mole (63.4 kcal/mole), respectively. These results compare favorably to water transport parameters in whole liver tissue reported in the first part of this study obtained using a freeze substitution (FS) microscopy technique (Pazhayannur and Bischof, 1997). The DSC technique is shown to be a fast, quantitative, and reproducible technique to measure dynamic water transport in tissue systems. However, there are several limitations to the DSC technique: (a) a priori knowledge that the biophysical response is in fact water transport, (b) the technique cannot be used due to machine limitations at cooling rates greater than 40°C/min, and (c) the tissue geometric dimensions (the Krogh model dimensions) and the osmotically inactive cell volumes Vb, must be determined by low-temperature microscopy techniques.

Highly selective two-photon fluorescence off-on probe for imaging tyrosinase activity in living cells and tissues

2021 ◽  
Author(s):  
Yixin Cui ◽  
Sang Jun Park ◽  
Xiaofeng Wu ◽  
Rui Wang ◽  
Sujie Qi ◽  
...  
Keyword(s):  
High Selectivity ◽  
Living Cells ◽  
Tyrosinase Activity ◽  
Live Cells ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A coumarin-based two-photon (TP) fluorescence off-on probe has been developed for detecting tyrosinase activity. High selectivity, sensitivity and biocompatibility enables probe to successfully image tyrosinase activity in live cells and...

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