scholarly journals Characterization and lymphocyte proliferation activity of an oligosaccharide degraded from Astragalus polysaccharide

MedChemComm ◽  
2017 ◽  
Vol 8 (7) ◽  
pp. 1521-1530 ◽  
Author(s):  
Zhen-Yuan Zhu ◽  
Jin-Yu Zhang ◽  
Fei Liu ◽  
Ling Chen ◽  
Li-Jing Chen ◽  
...  
Keyword(s):  
Lymphocyte Proliferation ◽  
Gel Chromatography ◽  
Membrane Dialysis ◽  
Astragalus Polysaccharide ◽  
Proliferation Activity ◽  
Silicon Gel

An Astragalus oligosaccharide (AOS) degraded from Astragalus polysaccharide (APS) and purified by membrane dialysis and silicon gel chromatography is studied in this paper.

Impaired cytokine production and suppressed lymphocyte proliferation activity in HCV-infected cocaine and heroin (“speedball”) users

2006 ◽  
Vol 85 (3) ◽  
pp. 236-243 ◽  
Author(s):  
Eddy Ríos-Olivares ◽  
Luis M. Vilá ◽  
Juan C. Reyes ◽  
José W. Rodríguez ◽  
J. Héctor M. Colón ◽  
...  
Keyword(s):  
Lymphocyte Proliferation ◽  
Cytokine Production ◽  
Proliferation Activity

scholarly journals IMMUNOSTIMULATORY EFFECT OF FATTY ACID FROM STAR FISH (ACANTHASTER PLANCI) ON LYMPHOCYTE PROLIFERATION IN-VITRO

2014 ◽  
Vol 9 (3) ◽  
pp. 107 ◽  
Author(s):  
M. Janib Achmad ◽  
Alim Isnansetyo ◽  
Noer Kasanah ◽  
Ustadi Ustadi ◽  
Kamiso Kamiso
Keyword(s):  
Fatty Acid ◽  
Lymphocyte Proliferation ◽  
Stimulation Index ◽  
Chemical Constituents ◽  
Gas Chromatography Mass Spectrometry ◽  
Acanthaster Planci ◽  
Proliferation Activity ◽  
And Control ◽  
Layer Chromatography

The aims of this study were to investigate lymphocyte proliferation activity and to identify chemical constituents of active fractions of star fish Acanthaster planci. A. planci was collected from Ternate Island, North Moluccas, extracted with distilled methanol and water, partitioned with gradient chloroform-hexane-methanol-water and fractionated in column chromatography using silica gel and hexane-ethyl acetate-methanol. The active compound had been purified by Thin Layer Chromatography (TLC) and identified by Gas Chromatography-Mass Spectrometry (GC-MS). The lymphocyte proliferation activity was measured based on % Stimulation Index (SI) from sample absorbency and control absorbency. The result showed that the 3 fractions of hexane fraction exhibited lymphocyte proliferation activity. Fraction 1 was able to increase lymphocyte proliferation at 48 hours and 72 hours by 55% (80 µl/ml) and 88% (160 µl/ml) increase, and fraction 2 had 77% (160 µl/ml) and 86% (640 µl/ml) increase. Meanwhile, fraction 3 had 75% (640 µl/ml) and 89% (640 µl/ml) increase. Metabolite analysis of active fraction using GC-MS yielded a number of chemical constituents that was dominated by fatty acid. The study concluded that star fish A. planci from Ternate Island has a potential source of immunostimulator.

scholarly journals THE EFFECTIVENESS OF SNAIL MUCUS (ACHATINA FULICA) AND CHITOSAN TOWARD LIMFOSIT PROLIFERATION IN VITRO

2018 ◽  
Vol 11 (15) ◽  
pp. 85 ◽  
Author(s):  
Agnes Sri Harti ◽  
Atiek Murharyati ◽  
Dwi Sulisetyawati S ◽  
Meri Oktariani
Keyword(s):  
Cell Proliferation ◽  
Lymphocyte Proliferation ◽  
Positive Control ◽  
Negative Control ◽  
Diphenyltetrazolium Bromide ◽  
Proliferation Activity ◽  
Mtt Reduction ◽  
Lymphocyte Cell ◽  
Effective Result

Objective: The objective of this research was to assess the effectiveness of mucous and chitin slime cream against lymphocyte proliferation in vitro.Methods: The research methods include snail mucus isolation, snail mucus cream material preparation, and lymphocyte cell proliferation test of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction method.Results: Lymphocyte cell proliferation test of MTT reduction method showed that chitosan 5% gave the most effective result toward lymphocyte proliferation activity compared to 100% snail slime and 5% snail slime cream. The 5% snail mucus cream 5% provides a higher proliferative activity than the 100% snail mucus. Positive control using Con A solution. Negative control was treatment without addition of test solution. The differences in lymphocyte proliferation activity were due to the presence of active compounds in chitosan, snail mucus, and snail slime cream that potentially increase lymphocyte proliferation. Significant differences in lymphocyte cell proliferation as Group K1, K2, K3, K4, K5, and K6.Conclusion: The effectiveness of snail and chitosan slime cream toward lymphocyte proliferation by in vitro that chitosan 5% gave the most effective result toward lymphocyte proliferation activity compared to 100% snail slime and 5% snail slime cream.

Effects of Astragalus polysaccharide liposome on lymphocyte proliferation in vitro and adjuvanticity in vivo

2012 ◽  
Vol 88 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Yunpeng Fan ◽  
Yuanliang Hu ◽  
Deyun Wang ◽  
Jiaguo Liu ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Lymphocyte Proliferation ◽  
Astragalus Polysaccharide ◽  
Proliferation In Vitro

scholarly journals Experimental immunology Lymphocyte proliferation activity after limited (Light class) and long (CEI) distance endurance rides in horses

2012 ◽  
Vol 4 ◽  
pp. 326-331 ◽  
Author(s):  
Anna Cywińska ◽  
Ewa Szarska ◽  
Renata Górecka ◽  
Lucjan Witkowski ◽  
Zuzanna Wyszyńska ◽  
...  
Keyword(s):  
Lymphocyte Proliferation ◽  
Proliferation Activity ◽  
Experimental Immunology

scholarly journals Lymphocyte Proliferation Activity MTT -Test of Ethanolic Extract of Pasak Bumi Root (Eurycoma longifolia Jack) On Induced 7,12-Dimethylbenz[A] Antracene (DMBA) Female Sprague Dawley Rat

2011 ◽  
Vol 2 (1) ◽  
pp. 187
Author(s):  
Lily Noor Falah ◽  
Sapto Yuliani
Keyword(s):  
Lymphocyte Proliferation ◽  
Relative Weight ◽  
Ethanol Extract ◽  
Sprague Dawley ◽  
Mtt Test ◽  
Eurycoma Longifolia ◽  
Sprague Dawley Rat ◽  
Group I ◽  
Proliferation Activity ◽  
Eurycoma Longifolia Jack

Pasak bumi root (Eurycoma longifolia Jack) contains quasinoid. Quasinoid potentially has antitumor/anticancer, anti-parasitic, and immunostimulatory activity. The aim of this research was to study the lymphocyte proliferation activity MTT-test of ethanol extract of Eurycoma longifolia Jack root on induced by 7,12-dimethylbenz[a] antracene (DMBA) female Sprague Dawley rat. The test was done at 6 groups of 10 SD rat each. Each groups was administered orally with DMBA (Group I) dose of 20 mg/kg bw, corn oil (Group II), baseline (Group III), and  ethanol extract of pasak bumi root dose 12.6; 25.2; and 50.4 mg/kg bw (Group IV,V,and VI). Ethanol extract is given for 5 weeks. At the second week five rats were sacrificed to isolate the lymphocytes of spleen then rats are injected orally with DMBA 2 times a week for 5 weeks. At the eight until twenty fourth week all groups were just administered by aquades. Measurement of spleen relative weight and isolation of the lymphocyte spleen were done at second week and the last of experiment. Lymphocyte proliferation activity were measured by MTT-reduction method. Data were analyzed statistically by analyzes of variance (anova) continued by LSD test and Kruskall Wallis continued by Mann Whitney test. The result showed that limphocyte proliferation activity MTT of ethanol extract of E. longifolia root doses of 12.6; 25.2; and 50.4 mg/kg bw for 14 days prior to DMBA-induced are  339.35 ± 8.43;61.20 ± 6.27;310.28 ±  6.81   and  dose 50,4 mg/kg bw after  DMBA-induced is 122.108 ±  11.426 .Keyword: E. longifolia root, spleen,  lymphocyte cell, 7,12-Dimetilbenz (a) antracene (DMBA)

Sign in / Sign up

Export Citation Format

Share Document