Enzymatic hydrolysis optimization ofPaphia undulataand lymphocyte proliferation activity of the isolated peptide fractions

2014 ◽  
Vol 95 (7) ◽  
pp. 1544-1553 ◽  
Author(s):  
Xiao Qing He ◽  
Wen Hong Cao ◽  
Guang Kun Pan ◽  
Liu Yang ◽  
Chao Hua Zhang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lymphocyte Proliferation ◽  
Proliferation Activity ◽  
Peptide Fractions

scholarly journals Characterization and lymphocyte proliferation activity of an oligosaccharide degraded from Astragalus polysaccharide

MedChemComm ◽  
2017 ◽  
Vol 8 (7) ◽  
pp. 1521-1530 ◽  
Author(s):  
Zhen-Yuan Zhu ◽  
Jin-Yu Zhang ◽  
Fei Liu ◽  
Ling Chen ◽  
Li-Jing Chen ◽  
...  
Keyword(s):  
Lymphocyte Proliferation ◽  
Gel Chromatography ◽  
Membrane Dialysis ◽  
Astragalus Polysaccharide ◽  
Proliferation Activity ◽  
Silicon Gel

An Astragalus oligosaccharide (AOS) degraded from Astragalus polysaccharide (APS) and purified by membrane dialysis and silicon gel chromatography is studied in this paper.

Production of Interesting Peptide Fractions by Enzymatic Hydrolysis of Tuna Dark Muscle By-Product Using Alcalase

2014 ◽  
Vol 25 (2) ◽  
pp. 251-264 ◽  
Author(s):  
Sami Saidi ◽  
Marie-Pierre Belleville ◽  
André Deratani ◽  
Raja Ben Amar
Keyword(s):  
Enzymatic Hydrolysis ◽  
Dark Muscle ◽  
Hydrolysis Of ◽  
Peptide Fractions

Characteristics of Enzymatic Hydrolysis of the Wheat Gluten Proteins Treated by Ultrasound Wave

2011 ◽  
Vol 343-344 ◽  
pp. 1015-1022
Author(s):  
Hua Li Jin ◽  
Jin Shui Wang ◽  
Ke Bian
Keyword(s):  
Enzymatic Hydrolysis ◽  
Power Output ◽  
Wheat Gluten ◽  
Protein Recovery ◽  
Degree Of Hydrolysis ◽  
Ultrasound Wave ◽  
Gluten Proteins ◽  
Hydrolysis Of ◽  
Peptide Fractions ◽  
Large Peptide

Effects of sonication at 150W and 300W power output on hydrolysis of wheat gluten using two proteinases (Protamex and papain) were evaluated in the present study. Sonication resulted in the increase in amounts of free sulphydryl (SH) of wheat gluten. Significant (P < 0.05) increase in SH contents at 300W power output was found compared with the control and those samples sonicated at 150W power output. Degree of hydrolysis (DH) and protein recovery (PR) of the wheat gluten hydrolysates increased with sonication time and power output. Significant (P < 0.05) increase in DH and PR of the wheat gluten hydrolysate from the sample sonicated by 300W power output was found compared to those sonicated by 150W output. Sonication caused the decrease in the relative percent of the large peptide fractions and increase in the relative percent of the small peptide fractions in the hydrolysates compared to the control. Structure change in insoluble wheat gluten proteins was the principal reason of improvement in their characteristics of enzymatic hydrolysis.

Impaired cytokine production and suppressed lymphocyte proliferation activity in HCV-infected cocaine and heroin (“speedball”) users

2006 ◽  
Vol 85 (3) ◽  
pp. 236-243 ◽  
Author(s):  
Eddy Ríos-Olivares ◽  
Luis M. Vilá ◽  
Juan C. Reyes ◽  
José W. Rodríguez ◽  
J. Héctor M. Colón ◽  
...  
Keyword(s):  
Lymphocyte Proliferation ◽  
Cytokine Production ◽  
Proliferation Activity

Immunoreactive properties of peptide fractions of cow whey milk proteins after enzymatic hydrolysis

2004 ◽  
Vol 39 (8) ◽  
pp. 839-850 ◽  
Author(s):  
Barbara Wroblewska ◽  
Magdalena Karamac ◽  
Ryszard Amarowicz ◽  
Agata Szymkiewicz ◽  
Agnieszka Troszynska ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Milk Proteins ◽  
Peptide Fractions

scholarly journals IMMUNOSTIMULATORY EFFECT OF FATTY ACID FROM STAR FISH (ACANTHASTER PLANCI) ON LYMPHOCYTE PROLIFERATION IN-VITRO

2014 ◽  
Vol 9 (3) ◽  
pp. 107 ◽  
Author(s):  
M. Janib Achmad ◽  
Alim Isnansetyo ◽  
Noer Kasanah ◽  
Ustadi Ustadi ◽  
Kamiso Kamiso
Keyword(s):  
Fatty Acid ◽  
Lymphocyte Proliferation ◽  
Stimulation Index ◽  
Chemical Constituents ◽  
Gas Chromatography Mass Spectrometry ◽  
Acanthaster Planci ◽  
Proliferation Activity ◽  
And Control ◽  
Layer Chromatography

The aims of this study were to investigate lymphocyte proliferation activity and to identify chemical constituents of active fractions of star fish Acanthaster planci. A. planci was collected from Ternate Island, North Moluccas, extracted with distilled methanol and water, partitioned with gradient chloroform-hexane-methanol-water and fractionated in column chromatography using silica gel and hexane-ethyl acetate-methanol. The active compound had been purified by Thin Layer Chromatography (TLC) and identified by Gas Chromatography-Mass Spectrometry (GC-MS). The lymphocyte proliferation activity was measured based on % Stimulation Index (SI) from sample absorbency and control absorbency. The result showed that the 3 fractions of hexane fraction exhibited lymphocyte proliferation activity. Fraction 1 was able to increase lymphocyte proliferation at 48 hours and 72 hours by 55% (80 µl/ml) and 88% (160 µl/ml) increase, and fraction 2 had 77% (160 µl/ml) and 86% (640 µl/ml) increase. Meanwhile, fraction 3 had 75% (640 µl/ml) and 89% (640 µl/ml) increase. Metabolite analysis of active fraction using GC-MS yielded a number of chemical constituents that was dominated by fatty acid. The study concluded that star fish A. planci from Ternate Island has a potential source of immunostimulator.

scholarly journals Antioxidant, ACE-Inhibitory and Antimicrobial Activities of Kluyveromyces marxianus Protein Hydrolysates and Their Peptide Fractions

2016 ◽  
Vol 6 (7) ◽  
pp. 425 ◽  
Author(s):  
Mahta Mirzaei ◽  
Mahmoud Aminlari ◽  
Ebrahim Hosseini
Keyword(s):  
Enzymatic Hydrolysis ◽  
Antimicrobial Peptides ◽  
Kluyveromyces Marxianus ◽  
Protein Hydrolysate ◽  
Radical Scavenging ◽  
Protein Hydrolysates ◽  
Antimicrobial Protein ◽  
Degree Of Hydrolysis ◽  
Peptide Fractions ◽  
Ace Inhibitory

Background: There has been some evidence that proteins are potentially excellent source of antioxidants, antihypertensive and antimicrobial peptides and enzymatic hydrolysis is an effective method to release these peptides from protein molecules. The functional properties of protein hydrolysates depends on the protein substrate, the specificity of the enzymes, the conditions used during proteolysis, degree of hydrolysis, and the nature of peptides released including molecular weight, amino acid composition, and hydrophobicity.Context and purpose of this study: The biomass of Kluyveromyces marxianus was considered as a source of ACE inhibitory, antioxidant and antimicrobial peptides. Results: Autolysis and enzymatic hydrolysis were completed respectively, after 96 h and 5 h. Overall, trypsin (18.52% DH) and chymotrypsin (21.59% DH) treatments were successful in releasing antioxidant and ACE inhibitory peptides. Autolysate sample (39.51% DH) demonstrated a poor antioxidant and ACE inhibitory activity compared to trypsin and chymotrypsin hydrolysates. The chymotrypsin 3-5 kDa (301.6±22.81 μM TEAC/mg protein) and trypsin< 3 kDa (280.16±39.16) permeate peptide fractions showed the highest DPPH radical scavenging activity. The trypsin <3 kDa permeate peptide fraction showed the highest ABTS radical scavenging (1691.1±48.68 μMTE/mg protein) and ACE inhibitory (IC50=0.03±0.001 mg/ml) activities. The fraction (MW=5-10 kD) obtained after autolysis treatment showed antibacterial activity against St. aureus and Lis. monocytogenes in well diffusion screening. The minimum inhibitory concentration (MIC) value was 13.3 mg/ml against St. aureus and Lis. monocytogenes calculated by turbidimetric assay and it showed bactericidal activity against St. aureus at 21.3 mg/ml protein concentration. Conclusions: Taken together, the results of this study reveal that K. marxianus proteins contain specific peptides in their sequences which can be released by enzymatic hydrolysis and autolysis. Key words: Kluyveromyces marxianus; Antioxidant activity; ACE-inhibitory; Antimicrobial; Protein hydrolysate; Peptide 

scholarly journals THE EFFECTIVENESS OF SNAIL MUCUS (ACHATINA FULICA) AND CHITOSAN TOWARD LIMFOSIT PROLIFERATION IN VITRO

2018 ◽  
Vol 11 (15) ◽  
pp. 85 ◽  
Author(s):  
Agnes Sri Harti ◽  
Atiek Murharyati ◽  
Dwi Sulisetyawati S ◽  
Meri Oktariani
Keyword(s):  
Cell Proliferation ◽  
Lymphocyte Proliferation ◽  
Positive Control ◽  
Negative Control ◽  
Diphenyltetrazolium Bromide ◽  
Proliferation Activity ◽  
Mtt Reduction ◽  
Lymphocyte Cell ◽  
Effective Result

Objective: The objective of this research was to assess the effectiveness of mucous and chitin slime cream against lymphocyte proliferation in vitro.Methods: The research methods include snail mucus isolation, snail mucus cream material preparation, and lymphocyte cell proliferation test of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction method.Results: Lymphocyte cell proliferation test of MTT reduction method showed that chitosan 5% gave the most effective result toward lymphocyte proliferation activity compared to 100% snail slime and 5% snail slime cream. The 5% snail mucus cream 5% provides a higher proliferative activity than the 100% snail mucus. Positive control using Con A solution. Negative control was treatment without addition of test solution. The differences in lymphocyte proliferation activity were due to the presence of active compounds in chitosan, snail mucus, and snail slime cream that potentially increase lymphocyte proliferation. Significant differences in lymphocyte cell proliferation as Group K1, K2, K3, K4, K5, and K6.Conclusion: The effectiveness of snail and chitosan slime cream toward lymphocyte proliferation by in vitro that chitosan 5% gave the most effective result toward lymphocyte proliferation activity compared to 100% snail slime and 5% snail slime cream.

scholarly journals Experimental immunology Lymphocyte proliferation activity after limited (Light class) and long (CEI) distance endurance rides in horses

2012 ◽  
Vol 4 ◽  
pp. 326-331 ◽  
Author(s):  
Anna Cywińska ◽  
Ewa Szarska ◽  
Renata Górecka ◽  
Lucjan Witkowski ◽  
Zuzanna Wyszyńska ◽  
...  
Keyword(s):  
Lymphocyte Proliferation ◽  
Proliferation Activity ◽  
Experimental Immunology
Sign in / Sign up

Export Citation Format

Share Document