Stretchable impedance sensor for mammalian cell proliferation measurements

Lab on a Chip ◽  
2017 ◽  
Vol 17 (12) ◽  
pp. 2054-2066 ◽  
Author(s):  
Xudong Zhang ◽  
William Wang ◽  
Fang Li ◽  
Ioana Voiculescu
Keyword(s):  
Cell Proliferation ◽  
Mammalian Cell ◽  
Lab On A Chip ◽  
Impedance Sensing ◽  
Cell Substrate ◽  
Impedance Sensor

This paper presents the fabrication and testing of a novel stretchable electric cell–substrate impedance sensing (ECIS) lab on a chip device.

scholarly journals The Measurement and Analysis of Impedance Response of HeLa Cells to Distinct Chemotherapy Drugs

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 202
Author(s):  
Xiangbin Du ◽  
Jinlong Kong ◽  
Yang Liu ◽  
Qianmin Xu ◽  
Kaiqun Wang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Hela Cells ◽  
Electrode System ◽  
Evaluation Methodology ◽  
Label Free ◽  
Impedance Sensing ◽  
Chemotherapy Drugs ◽  
Substrate Adhesion ◽  
Cell Substrate ◽  
Cell Electrode

Electric cell–substrate impedance sensing exhibits a real-time and label-free feature to monitor the response of cells stimulated by various biochemical and mechanical signals. Alterations in the currents passing through the cell–electrode system characterize the impedance variations of cells. The impedance responses of HeLa cells under distinct chemotherapy drugs combine the effects of cell proliferation and cell–substrate adhesion. Optimal interdigitated electrodes were selected to explore the impedance responses of HeLa cells. Measurements of impedance of cells in response to three widely used chemotherapy drugs in clinical practice, namely cisplatin, doxorubicin, 5-fluorouracil, were performed. The results demonstrated that distinct impedance responses of HeLa cells to drugs were exhibited and a decrease in measured impedance was observed after drug treatment, accompanied by alterations in the distribution and intensity of the adhesion-related protein vinculin and the rate of cell proliferation. The link between the impedance profiles of HeLa cells and their biological functions was developed based on the circuit model. This study demonstrated the weights of cell proliferation and adhesion of HeLa cells under the treatments of DDP, DOX, and 5-FU, resulted in distinct impedance responses of cells, providing an impedance-based evaluation methodology for cervical cancer treatment.

scholarly journals Electric Cell-Substrate Impedance Sensing To Monitor Viral Growth and Study Cellular Responses to Infection with Alphaherpesviruses in Real Time

mSphere ◽  
2017 ◽  
Vol 2 (2) ◽  
Author(s):  
Matthew R. Pennington ◽  
Gerlinde R. Van de Walle
Keyword(s):  
Real Time ◽  
Cellular Response ◽  
Cellular Responses ◽  
Cellular Damage ◽  
Impedance Sensing ◽  
Novel Technologies ◽  
Antiviral Therapies ◽  
Mucosal Surfaces ◽  
Cell Substrate ◽  
Hsv 1

ABSTRACT Alphaherpesviruses, including those that commonly infect humans, such as HSV-1 and HSV-2, typically infect and cause cellular damage to epithelial cells at mucosal surfaces, leading to disease. The development of novel technologies to study the cellular responses to infection may allow a more complete understanding of virus replication and the creation of novel antiviral therapies. This study demonstrates the use of ECIS to study various aspects of herpesvirus biology, with a specific focus on changes in cellular morphology as a result of infection. We conclude that ECIS represents a valuable new tool with which to study alphaherpesvirus infections in real time and in an objective and reproducible manner. Electric cell-substrate impedance sensing (ECIS) measures changes in an electrical circuit formed in a culture dish. As cells grow over a gold electrode, they block the flow of electricity and this is read as an increase in electrical impedance in the circuit. ECIS has previously been used in a variety of applications to study cell growth, migration, and behavior in response to stimuli in real time and without the need for cellular labels. Here, we demonstrate that ECIS is also a valuable tool with which to study infection by alphaherpesviruses. To this end, we used ECIS to study the kinetics of cells infected with felid herpesvirus type 1 (FHV-1), a close relative of the human alphaherpesviruses herpes simplex virus 1 (HSV-1) and HSV-2, and compared the results to those obtained with conventional infectivity assays. First, we demonstrated that ECIS can easily distinguish between wells of cells infected with different amounts of FHV-1 and provides information about the cellular response to infection. Second, we found ECIS useful in identifying differences between the replication kinetics of recombinant DsRed Express2-labeled FHV-1, created via CRISPR/Cas9 genome engineering, and wild-type FHV-1. Finally, we demonstrated that ECIS can accurately determine the half-maximal effective concentration of antivirals. Collectively, our data show that ECIS, in conjunction with current methodologies, is a powerful tool that can be used to monitor viral growth and study the cellular response to alphaherpesvirus infection. IMPORTANCE Alphaherpesviruses, including those that commonly infect humans, such as HSV-1 and HSV-2, typically infect and cause cellular damage to epithelial cells at mucosal surfaces, leading to disease. The development of novel technologies to study the cellular responses to infection may allow a more complete understanding of virus replication and the creation of novel antiviral therapies. This study demonstrates the use of ECIS to study various aspects of herpesvirus biology, with a specific focus on changes in cellular morphology as a result of infection. We conclude that ECIS represents a valuable new tool with which to study alphaherpesvirus infections in real time and in an objective and reproducible manner.

Bacterially produced inclusion bodies as biocompatible materials for substrate-dependent mammalian cell proliferation

2010 ◽  
Vol 150 ◽  
pp. 434-435
Author(s):  
J. Seras ◽  
C. Díez-Gil ◽  
E. Vazquez ◽  
S. Krabbenborg ◽  
E. Rodríguez-Carmona ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Mammalian Cell ◽  
Inclusion Bodies ◽  
Biocompatible Materials

Electrical cell-substrate impedance sensing as a non-invasive tool for cancer cell study

The Analyst ◽  
2011 ◽  
Vol 136 (2) ◽  
pp. 237-245 ◽  
Author(s):  
Jongin Hong ◽  
Karthikeyan Kandasamy ◽  
Mohana Marimuthu ◽  
Cheol Soo Choi ◽  
Sanghyo Kim
Keyword(s):  
Cancer Cell ◽  
Impedance Sensing ◽  
Non Invasive ◽  
Cell Substrate ◽  
Cell Study

scholarly journals Comparison of Leading Biosensor Technologies to Detect Changes in Human Endothelial Barrier Properties in Response to Pro-Inflammatory TNFα and IL1β in Real-Time

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 159
Author(s):  
James J. W. Hucklesby ◽  
Akshata Anchan ◽  
Simon J. O'Carroll ◽  
Charles P. Unsworth ◽  
E. Scott Graham ◽  
...  
Keyword(s):  
Human Brain ◽  
Real Time ◽  
Barrier Properties ◽  
Endothelial Barrier ◽  
Endothelial Monolayer ◽  
Impedance Sensing ◽  
Endothelial Monolayers ◽  
Impedance Data ◽  
Cell Substrate ◽  
Limited Frequency

Electric Cell-Substrate Impedance Sensing (ECIS), xCELLigence and cellZscope are commercially available instruments that measure the impedance of cellular monolayers. Despite widespread use of these systems individually, direct comparisons between these platforms have not been published. To compare these instruments, the responses of human brain endothelial monolayers to TNFα and IL1β were measured on all three platforms simultaneously. All instruments detected transient changes in impedance in response to the cytokines, although the response magnitude varied, with ECIS being the most sensitive. ECIS and cellZscope were also able to attribute responses to particular endothelial barrier components by modelling the multifrequency impedance data acquired by these instruments; in contrast the limited frequency xCELLigence data cannot be modelled. Consistent with its superior impedance sensing, ECIS exhibited a greater capacity than cellZscope to distinguish between subtle changes in modelled endothelial monolayer properties. The reduced resolving ability of the cellZscope platform may be due to its electrode configuration, which is necessary to allow access to the basolateral compartment, an important advantage of this instrument. Collectively, this work demonstrates that instruments must be carefully selected to ensure they are appropriate for the experimental questions being asked when assessing endothelial barrier properties.

Electric cell–substrate impedance sensing technique to monitor cellular behaviours of cancer cells

RSC Advances ◽  
2014 ◽  
Vol 4 (19) ◽  
pp. 9432 ◽  
Author(s):  
Rangadhar Pradhan ◽  
Shashi Rajput ◽  
Mahitosh Mandal ◽  
Analava Mitra ◽  
Soumen Das
Keyword(s):  
Cancer Cells ◽  
Impedance Sensing ◽  
Cell Substrate

Role of Intracellular pH in Mammalian Cell Proliferation

1992 ◽  
Vol 2 (3-4) ◽  
pp. 159-179 ◽  
Author(s):  
R.J. Gillies ◽  
R. Martinez-Zaguilan ◽  
E.P. Peterson ◽  
R. Perona
Keyword(s):  
Cell Proliferation ◽  
Intracellular Ph ◽  
Mammalian Cell
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