Ligand-inducible dimeric antibody for selecting antibodies against a membrane protein based on mammalian cell proliferation

2015 ◽  
Vol 113 (5) ◽  
pp. 1113-1123 ◽  
Author(s):  
Tomohiro Miura ◽  
Teruyuki Nagamune ◽  
Masahiro Kawahara
Keyword(s):  
Cell Proliferation ◽  
Membrane Protein ◽  
Mammalian Cell

Bacterially produced inclusion bodies as biocompatible materials for substrate-dependent mammalian cell proliferation

2010 ◽  
Vol 150 ◽  
pp. 434-435
Author(s):  
J. Seras ◽  
C. Díez-Gil ◽  
E. Vazquez ◽  
S. Krabbenborg ◽  
E. Rodríguez-Carmona ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Mammalian Cell ◽  
Inclusion Bodies ◽  
Biocompatible Materials

scholarly journals YME1L controls the accumulation of respiratory chain subunits and is required for apoptotic resistance, cristae morphogenesis, and cell proliferation

2012 ◽  
Vol 23 (6) ◽  
pp. 1010-1023 ◽  
Author(s):  
Lukas Stiburek ◽  
Jana Cesnekova ◽  
Olga Kostkova ◽  
Daniela Fornuskova ◽  
Kamila Vinsova ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Membrane Protein ◽  
Respiratory Chain ◽  
Integral Membrane Protein ◽  
Intermembrane Space ◽  
Wild Type ◽  
Inner Membrane ◽  
Mitochondrial Inner Membrane ◽  
Human Orthologue ◽  
Excessive Accumulation

Mitochondrial ATPases associated with diverse cellular activities (AAA) proteases are involved in the quality control and processing of inner-membrane proteins. Here we investigate the cellular activities of YME1L, the human orthologue of the Yme1 subunit of the yeast i‑AAA complex, using stable short hairpin RNA knockdown and expression experiments. Human YME1L is shown to be an integral membrane protein that exposes its carboxy-terminus to the intermembrane space and exists in several complexes of 600–1100 kDa. The stable knockdown of YME1L in human embryonic kidney 293 cells led to impaired cell proliferation and apoptotic resistance, altered cristae morphology, diminished rotenone-sensitive respiration, and increased susceptibility to mitochondrial membrane protein carbonylation. Depletion of YME1L led to excessive accumulation of nonassembled respiratory chain subunits (Ndufb6, ND1, and Cox4) in the inner membrane. This was due to a lack of YME1L proteolytic activity, since the excessive accumulation of subunits was reversed by overexpression of wild-type YME1L but not a proteolytically inactive YME1L variant. Similarly, the expression of wild-type YME1L restored the lamellar cristae morphology of YME1L-deficient mitochondria. Our results demonstrate the importance of mitochondrial inner-membrane proteostasis to both mitochondrial and cellular function and integrity and reveal a novel role for YME1L in the proteolytic regulation of respiratory chain biogenesis.

Role of Intracellular pH in Mammalian Cell Proliferation

1992 ◽  
Vol 2 (3-4) ◽  
pp. 159-179 ◽  
Author(s):  
R.J. Gillies ◽  
R. Martinez-Zaguilan ◽  
E.P. Peterson ◽  
R. Perona
Keyword(s):  
Cell Proliferation ◽  
Intracellular Ph ◽  
Mammalian Cell

scholarly journals Long-range memory of growth and cycle progression correlates cell cycles in lineage trees

2018 ◽  
Author(s):  
Erika E Kuchen ◽  
Nils Becker ◽  
Nina Claudino ◽  
Thomas Höfer
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Cell Growth ◽  
Mammalian Cell ◽  
Latent Variable ◽  
Cycle Length ◽  
Growth Control ◽  
Minimum Size ◽  
Cell Cycles ◽  
Lineage Trees

Mammalian cell proliferation is controlled by mitogens. However, how proliferation is coordinated with cell growth is poorly understood. Here we show that statistical properties of cell lineage trees – the cell-cycle length correlations within and across generations – reveal how cell growth controls proliferation. Analyzing extended lineage trees with latent-variable models, we find that two antagonistic heritable variables account for the observed cycle-length correlations. Using molecular perturbations of mTOR and MYC we identify these variables as cell size and regulatory license to divide, which are coupled through a minimum-size checkpoint. The checkpoint is relevant only for fast cell cycles, explaining why growth control of mammalian cell proliferation has remained elusive. Thus, correlated fluctuations of the cell cycle encode its regulation.

scholarly journals Enhancement of cell proliferation in various mammalian cell lines by gene insertion of a cyclin-dependent kinase homolog

2007 ◽  
Vol 7 (1) ◽  
pp. 71 ◽  
Author(s):  
Pratik Jaluria ◽  
Michael Betenbaugh ◽  
Konstantinos Konstantopoulos ◽  
Joseph Shiloach
Keyword(s):  
Cell Proliferation ◽  
Cell Lines ◽  
Mammalian Cell ◽  
Cyclin Dependent Kinase ◽  
Gene Insertion ◽  
Mammalian Cell Lines

Poly(3-hydroxybutyrate-co-valerate)/poly(3-thiophene ethyl acetate) blends as a electroactive biomaterial substrate for tissue engineering application

RSC Advances ◽  
2016 ◽  
Vol 6 (30) ◽  
pp. 25330-25338 ◽  
Author(s):  
M. S. Recco ◽  
A. C. Floriano ◽  
D. B. Tada ◽  
A. P. Lemes ◽  
R. Lang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Ethyl Acetate ◽  
Mammalian Cell ◽  
Engineering Application ◽  
Chemical Properties ◽  
Tissue Engineering Application ◽  
Good Adhesion ◽  
Physical Chemical ◽  
Low Cytotoxicity

Polyblend films based on poly(3-hydroxybutirate-co-valerate) and poly(3-thiophene ethyl acetate) – PHBV/PTAcEt showed low cytotoxicity, good adhesion and mammalian cell proliferation. The physical–chemical properties were explored.

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