scholarly journals On chip cryo-anesthesia of Drosophila larvae for high resolution in vivo imaging applications

Lab on a Chip ◽  
2017 ◽  
Vol 17 (13) ◽  
pp. 2303-2322 ◽  
Author(s):  
Amrita Ray Chaudhury ◽  
Ryan Insolera ◽  
Ran-Der Hwang ◽  
Yih-Woei Fridell ◽  
Catherine Collins ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
High Resolution ◽  
Microfluidic Chip ◽  
In Vivo Imaging ◽  
Drosophila Larvae ◽  
On Chip

We present a microfluidic chip for cryo-immobilizingDrosophila melanogasterlarvae for high resolutionin vivoimaging of neurobiological processes and other applications.

scholarly journals Clasp-mediated microtubule bundling regulates persistent motility and contact repulsion in Drosophila macrophages in vivo

2010 ◽  
Vol 189 (4) ◽  
pp. 681-689 ◽  
Author(s):  
Brian Stramer ◽  
Severina Moreira ◽  
Tom Millard ◽  
Iwan Evans ◽  
Chieh-Yin Huang ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
High Resolution ◽  
Fluorescent Probes ◽  
In Vivo Imaging ◽  
Living Organism ◽  
Developmental Guidance ◽  
Cytoskeletal Networks ◽  
Migratory Cells ◽  
Dynamic Interplay

Drosophila melanogaster macrophages are highly migratory cells that lend themselves beautifully to high resolution in vivo imaging experiments. By expressing fluorescent probes to reveal actin and microtubules, we can observe the dynamic interplay of these two cytoskeletal networks as macrophages migrate and interact with one another within a living organism. We show that before an episode of persistent motility, whether responding to developmental guidance or wound cues, macrophages assemble a polarized array of microtubules that bundle into a compass-like arm that appears to anticipate the direction of migration. Whenever cells collide with one another, their microtubule arms transiently align just before cell–cell repulsion, and we show that forcing depolymerization of microtubules by expression of Spastin leads to their defective polarity and failure to contact inhibit from one another. The same is true in orbit/clasp mutants, indicating a pivotal role for this microtubule-binding protein in the assembly and/or functioning of the microtubule arm during polarized migration and contact repulsion.

scholarly journals High-resolution in vivo imaging of rhesus cerebral cortex with ultrafast portable photoacoustic microscopy

NeuroImage ◽  
2021 ◽  
pp. 118260
Author(s):  
Wei Qin ◽  
Qi Gan ◽  
Lei Yang ◽  
Yongchao Wang ◽  
Weizhi Qi ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
High Resolution ◽  
In Vivo Imaging ◽  
Photoacoustic Microscopy

High-Resolution In Vivo Imaging in Achromatopsia

Ophthalmology ◽  
2011 ◽  
Vol 118 (5) ◽  
pp. 882-887 ◽  
Author(s):  
Mervyn G. Thomas ◽  
Anil Kumar ◽  
Susanne Kohl ◽  
Frank A. Proudlock ◽  
Irene Gottlob
Keyword(s):  
High Resolution ◽  
In Vivo Imaging

scholarly journals In Vivo Imaging Enables High Resolution Preclinical Trials on Patients’ Leukemia Cells Growing in Mice

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e52798 ◽  
Author(s):  
Nadia Terziyska ◽  
Catarina Castro Alves ◽  
Volker Groiss ◽  
Katja Schneider ◽  
Katarina Farkasova ◽  
...  
Keyword(s):  
High Resolution ◽  
In Vivo Imaging ◽  
Leukemia Cells ◽  
Preclinical Trials

scholarly journals On-chip immobilization of planarians for in vivo imaging

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Joseph P. Dexter ◽  
Mary B. Tamme ◽  
Christine H. Lind ◽  
Eva-Maria S. Collins
Keyword(s):  
In Vivo Imaging ◽  
On Chip

scholarly journals Ultra-high resolution adaptive optics: optical coherence tomography for in vivo imaging of healthy and diseased retinal structures

2008 ◽  
Author(s):  
Robert J. Zawadzki ◽  
Yan Zhang ◽  
Steven M. Jones ◽  
Stacey S. Choi ◽  
Barry Cense ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
High Resolution ◽  
Adaptive Optics ◽  
In Vivo Imaging ◽  
Optical Coherence

High-resolution in vivo imaging of blood vessels without labeling

2007 ◽  
Vol 32 (18) ◽  
pp. 2641 ◽  
Author(s):  
Dan Fu ◽  
Tong Ye ◽  
Thomas E. Matthews ◽  
Benny J. Chen ◽  
Gunay Yurtserver ◽  
...  
Keyword(s):  
High Resolution ◽  
Blood Vessels ◽  
In Vivo Imaging
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